- Urea cycle
The urea cycle (also known as the ornithine cycle) is a cycle of biochemical reactions occurring in many animals that produces urea ((NH2)2CO) from ammonia (NH3). This cycle was the first metabolic cycle discovered (Hans Krebs and Kurt Henseleit, 1932), five years before the discovery of the TCA cycle. In mammals, the urea cycle takes place primarily in the liver, and to a lesser extent in the kidney.
Organisms that cannot easily and quickly remove ammonia usually have to convert it to some other substance, like urea or uric acid, which are much less toxic. Insufficiency of the urea cycle occurs in some genetic disorders (inborn errors of metabolism), and in liver failure. The result of liver failure is accumulation of nitrogenous waste, mainly ammonia, which leads to hepatic encephalopathy.
The urea cycle consists of five reactions: two mitochondrial and three cytosolic. The cycle converts two amino groups, one from NH4+ and one from Asp, and a carbon atom from HCO3−, to the relatively nontoxic excretion product urea at the cost of four "high-energy" phosphate bonds (3 ATP hydrolyzed to 2 ADP and one AMP). Ornithine is the carrier of these carbon and nitrogen atoms.
Reactions of the urea cycle Step Reactants Products Catalyzed by Location 1 NH4+ + HCO3− + 2ATP carbamoyl phosphate + 2ADP + Pi CPS1 mitochondria 2 carbamoyl phosphate + ornithine citrulline + Pi OTC mitochondria 3 citrulline + aspartate + ATP argininosuccinate + AMP + PPi ASS cytosol 4 argininosuccinate Arg + fumarate ASL cytosol 5 Arg + H2O ornithine + urea ARG1 cytosol
- The reactions of the urea cycle
2 carbamoyl phosphate
CPS-1 carbamoyl phosphate synthetase I
OTC Ornithine transcarbamoylase
ASS argininosuccinate synthetase
ASL argininosuccinate lyase
ARG1 arginase 1
Thus, the overall equation of the urea cycle is:
Since fumarate is obtained by removing NH3 from aspartate (by means of reactions 3 and 4), and PPi + H2O → 2 Pi, the equation can be simplified as follows:
Note that reactions related to the urea cycle also cause the oxidation of 2 NADH, so the urea cycle releases slightly more energy than it consumes. These NADH are produced in two ways:
- One NADH molecule is reduced by the enzyme glutamate dehydrogenase in the conversion of glutamate to ammonium and α-ketoglutarate. Glutamate is the non-toxic carrier of amine groups. This provides the ammonium ion used in the initial synthesis of carbamoyl phosphate.
- The fumarate released in the cytosol is converted to malate by cytosolic fumarase. This malate is then converted to oxaloacetate by cytosolic malate dehydrogenase, generating a reduced NADH in the cytosol. Oxaloacetate is one of the keto acids preferred by transaminases, and so will be recycled to aspartate, maintained the flow of nitrogen into the urea cycle.
The two NADH produced can provide energy for the formation of 5 ATP, a net production of one high-energy phosphate bond for the urea cycle. However, if gluconeogenesis is underway in the cytosol, the latter reducing equivalent is used to drive the reversal of the GAPDH step instead of generating ATP.
The fate of oxaloacetate is either to produce aspartate via transamination or to be converted to phosphoenol pyruvate, which is a substrate to glucose.
The synthesis of carbamoyl phosphate and the urea cycle are dependent on the presence of NAcGlu, which allosterically activates CPS1. Synthesis of NAcGlu by NAGS, is stimulated by both Arg, allosteric stimulator of NAGS, and Glu, a product in the transamination reactions and one of NAGS's substrates, both of which are elevated when free amino acids are elevated. So, Arg is not only a substrate for the urea cycle reactions but also serves as an activator for the urea cycle.
The remaining enzymes of the cycle are controlled by the concentrations of their substrates. Thus, inherited deficiencies in the cycle enzymes other than ARG1 do not result in significant decrease in urea production (the total lack of any cycle enzyme results in death shortly after birth). Rather, the deficient enzyme's substrate builds up, increasing the rate of the deficient reaction to normal.
The anomalous substrate buildup is not without cost, however. The substrate concentrations become elevated all the way back up the cycle to NH4+, resulting in hyperammonemia (elevated [NH4+]P).
Although the root cause of NH4+ toxicity is not completely understood, a high [NH4+] puts an enormous strain on the NH4+-clearing system, especially in the brain (symptoms of urea cycle enzyme deficiencies include mental retardation and lethargy). This clearing system involves GLUD1 and GLUL, which decrease the 2-oxoglutarate (2OG) and Glu pools. The brain is most sensitive to the depletion of these pools. Depletion of 2OG decreases the rate of TCAC, whereas Glu is both a neurotransmitter and a precursor to GABA, another neurotransmitter. (p.734)
Anomalies of the urea cycle cause urea cycle disorders:
- ornithine transcarbamoylase deficiency
- Carbamoyl phosphate synthetase deficiency (Ornithine translocase deficiency)
- Argininosuccinic aciduria
- Hyperornithinemia, hyperammonemia, homocitrullinuria syndrome (HHH syndrome)
- Lysinuric protein intolerance
- N-Acetylglutamate synthase deficiency
Most of them are associated with hyperammonemia.
L-ornithine Pi L-aspartate Urea + ATP PPi + AMP H2O L-argininosuccinate Fumarate L-arginine Main cycle Regulatory/transport
Wikimedia Foundation. 2010.
Look at other dictionaries:
urea cycle — a series of metabolic reactions, occurring in the liver, by which ammonia is converted to urea using cyclically regenerated ornithine as a carrier. See illustration. Urea cycle. Diagrammatic representation of reactions by which excess nitrogen in … Medical dictionary
urea cycle — Biochem. a metabolic process by which ammonia derived from amino acids is converted into urea in the liver. Also called Krebs urea cycle. * * * … Universalium
urea cycle — Biochem. a metabolic process by which ammonia derived from amino acids is converted into urea in the liver. Also called Krebs urea cycle … Useful english dictionary
urea cycle — /juˈriə saɪkəl/ (say yooh reeuh suykuhl) noun a series of reactions in animals involving the conversion of ammonia, carbon dioxide and aspartic acid to urea and fumaric acid, for the excretion of urea and the production of arginine … Australian English dictionary
Urea cycle disorder — Infobox Disease Name = PAGENAME Caption = ammonia DiseasesDB = ICD10 = ICD10|E|72|2|e|70 ICD9 = ICD9|270.6 ICDO = OMIM = MedlinePlus = 000372 eMedicineSubj = eMedicineTopic = MeshID = An urea cycle disorder or urea cycle defect is a genetic… … Wikipedia
Krebs urea cycle. — See urea cycle. [after H.A. KREBS] * * * … Universalium
Krebs urea cycle. — See urea cycle. [after H.A. KREBS] … Useful english dictionary
Urea — Urea … Wikipedia
Cycle De L'urée — Le cycle de l urée ou cycle de l ornithine est un cycle de réactions biochimiques chez divers animaux qui produisent de l urée à partir de l ammoniaque. Il s agit du premier cycle métabolique identifié dès 1932 par Krebs et Hensenleit. Ce cycle… … Wikipédia en Français
Cycle de l'ornithine — Cycle de l urée Le cycle de l urée ou cycle de l ornithine est un cycle de réactions biochimiques chez divers animaux qui produisent de l urée à partir de l ammoniaque. Il s agit du premier cycle métabolique identifié dès 1932 par Krebs et… … Wikipédia en Français