- Ribosomal protein
A ribosomal protein is any of the proteins that, in conjunction with rRNA, make up the ribosomal subunits involved in the cellular process of translation. A large part of the knowledge about these organic molecules has come from the study of E. coli ribosomes. Most ribosomic proteins have been isolated and specific anti-bodies have been produced. These, together with electronic microscopy and the use of certain reactives, have allowed for the determination of the topography of the proteins in the ribosome.
Proteins in "E. coli" ribosomes
The ribosome of "E. coli" has about 22 proteins in the small subunit (labelled S1 to S22) and 34 proteins in the large subunit (L1 to L36). All of them are different with three exceptions: one protein is found in both subunits (S20 and L26), L7 and L12 are acetylated and methylated forms of the same protein, and L8 is a complex of L7/L12 and L10. In addition, L31 is known to exist in two forms, the full length at 7.9 kiloDaltons (kDa) and fragmented at 7.0 kDa. This is why the number of proteins in a ribosome is of 56. Except for S1 (with a molecular weight of 61.2 kDa), the other proteins range in weight between 4.4 and 29.7 kDa. [Arnold RJ, Reilly JP. (1999) "Observation of Escherichia coli ribosomal proteins and their posttranslational modifications by mass spectrometry." "Anal Biochem." v269(1): pp 105-12. PMID 10094780]
Disposition in the small ribosomal subunit
In the small (30S) subunit of E. coli ribosomes, the proteins denoted S4, S7, S8, S15, S17, S20 bind independently to 16S rRNA. After assembly of these primary binding proteins, S5, S6, S9, S12, S13, S16, S18, and S19 bind to the growing ribosome. These proteins also potentiate the addition of S2, S3, S10, S11, S14, and S21. Protein binding to helical junctions is important for initiating the correct tertiary fold of RNA and to organize the overall structure. Nearly all the proteins contain one or more globular domains. Moreover, nearly all contain long extensions that can contact the RNA in far-reaching regions. Additional stabilization results from the proteins' basic residues, as these neutralize the charge repulsion of the RNA backbone. Protein-protein interactions also exist to hold structure together by electrostatic and hydrogen bonding interactions. Theoretical investigations pointed to correlated effects of protein-binding onto binding affinities during the assembly process [Hamacher K, Trylska J, McCammon JA. (2006) "Dependency Map of Proteins in the Small Ribosomal Subunit." "PLoS Comput. Biol." v2(2): e10. PMID 16485038]
* De Robertis and De Robertis, "Biología Celular y Molecular" 10th ed., El Ateneo, Buenos Aires,
1982, ISBN 950-02-0027-9 (in Spanish)Monika Martick, Lucas H. Horan, Harry F. Noller and William G. Scott. A discontinuous hammerhead ribozyme embedded in a mammalian messenger RNA. doi:10.1038/nature07117, First paragraph | Full Text | PDF
* [http://www.biochem.umd.edu/biochem/kahn/bchm465-01/ribosome/proteins.html 30S Ribosomal proteins at biochem.umd.edu]
Wikimedia Foundation. 2010.
Look at other dictionaries:
Ribosomal protein SA — Ribosomal protein SA, also known as RPSA, is a human gene.cite web | title = Entrez Gene: RPSA ribosomal protein SA| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene Cmd=ShowDetailView TermToSearch=3921| accessdate = ] PBB Summary section… … Wikipedia
Ribosomal protein s6 — protein Name=ribosomal protein S6 caption= width= HGNCid=10429 Symbol=RPS6 AltSymbols= EntrezGene=6194 OMIM=180460 RefSeq=NM 001010 UniProt=P62753 PDB= ECnumber= Chromosome=9 Arm=p Band=21 LocusSupplementaryData=Ribosomal protein s6 is a… … Wikipedia
ribosomal protein — ribosomal protein. См. рибосомный белок. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
ribosomal protein — Proteins present within the ribosomal subunits. In prokaryotes there are 31 proteins in the large subunit and 21 in the small subunit. Eukaryotic subunits have 50 (large subunit) and 33 (small subunit) proteins … Dictionary of molecular biology
Ribosomal protein S19 — Ribosomal protein S19, also known as RPS19, is a human gene.cite web | title = Entrez Gene: RPS19 ribosomal protein S19| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene Cmd=ShowDetailView TermToSearch=6223| accessdate = ] PBB Summary… … Wikipedia
Ribosomal protein leader — A ribosomal protein leader is a mechanism used in cells to control the cellular concentraction of a protein that forms a part of the ribosome, and to make sure that the concentration is neither too high nor too low. [cite journal |author=Zengel… … Wikipedia
Ribosomal-protein-alanine N-acetyltransferase — In enzymology, a ribosomal protein alanine N acetyltransferase (EC number|18.104.22.168) is an enzyme that catalyzes the chemical reaction:acetyl CoA + ribosomal protein L alanine ightleftharpoons CoA + ribosomal protein N acetyl L alanineThus, the… … Wikipedia
Ribosomal protein L21 leader — This family is a putative ribosomal protein leader autoregulatory structure [cite journal | last = Zengel | first = JM | coauthors = Lindahl L | year = 1994 | title = Diverse mechanisms for regulating ribosomal protein synthesis in Escherichia… … Wikipedia
Ribosomal protein L13 leader — This family is a putative ribosomal protein leader autoregulatory structure [cite journal | last = Zengel | first = JM | coauthors = Lindahl L | year = 1994 | title = Diverse mechanisms for regulating ribosomal protein synthesis in Escherichia… … Wikipedia
Ribosomal protein L19 leader — This family is a putative ribosomal protein leader autoregulatory structure [cite journal | last = Zengel | first = JM | coauthors = Lindahl L | year = 1994 | title = Diverse mechanisms for regulating ribosomal protein synthesis in Escherichia… … Wikipedia