Creatinase

creatinase
Identifiers
EC number	3.5.3.3
CAS number	37340-58-2
Databases
IntEnz	IntEnz view
BRENDA	BRENDA entry
ExPASy	NiceZyme view
KEGG	KEGG entry
MetaCyc	metabolic pathway
PRIAM	profile
PDB structures	RCSB PDB PDBe PDBsum
Gene Ontology	AmiGO / EGO
Search PMC articles PubMed articles

Creatinase/Prolidase N-terminal domain
The Crystal Structure of the Creatinase/Prolidase N-terminal domain of an X-PRO dipeptidase from Streptococcus pyogenes to 1.85A [1]
Identifiers
Symbol	Creatinase_N
Pfam	PF01321
InterPro	IPR000587
SCOP	1chm
Available protein structures: Pfam structures PDB RCSB PDB; PDBe PDBsum structure summary

In enzymology, a creatinase (EC 3.5.3.3) is an enzyme that catalyzes the chemical reaction

creatine + H₂O $\rightleftharpoons$ sarcosine + urea

Thus, the two substrates of this enzyme are creatine and H₂O, whereas its two products are sarcosine and urea.

The native enzyme was shown to be made up of two subunit monomers via SDS-polyacrylamide gel electrophoresis. The molecular weights of these subunits was estimated to be 47,000 g/mol.^[2] The enzyme works as a homodimer, and is induced by choline chloride. Each monomer of creatinase has two clearly defined domains, a small N-terminal domain, and a large C-terminal domain. Each of the two active sites is made by residues of the large domain of one monomer and some residues of the small domain of the other monomer. It's been suggested that a sulfhydryl group is located on or near the active site of the enzyme following inhibition experiments. ^[2]. Creatinase has been found to be most active at pH 8 and is most stable between ph 6-8 for 24 hrs. at 37 degrees.^[2]

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amidines. The systematic name of this enzyme class is creatine amidinohydrolase. This enzyme participates in arginine and proline metabolism.

Structural studies

As of late 2007, two structures have been solved for this class of enzymes, with PDB accession codes 1CHM and 1KP0.

References

"RCSB Protein Data Bank - Structure Summary for 3O5V - The Crystal Structure of the Creatinase/Prolidase N-terminal domain of an X-PRO dipeptidase from Streptococcus pyogenes to 1.85A". http://www.rcsb.org/pdb/explore/explore.do?structureId=3O5V. 

1. ^ "RCSB Protein Data Bank - Structure Summary for 3O5V - The Crystal Structure of the Creatinase/Prolidase N-terminal domain of an X-PRO dipeptidase from Streptococcus pyogenes to 1.85A". http://www.rcsb.org/pdb/explore/explore.do?structureId=3O5V. 
2. ^ ^{a b c} Yoshimoto T, Oka I, Tsuru D (June 1976). "Purification, crystallization, and some properties of creatine amidinohydrolase from Pseudomonas putida". J. Biochem. 79 (6): 1381–3. PMID 8443. 

• ROCHE J, LACOMBE G, GIRARD H (1950). "[On the specificity of certain bacterial deguanidases generating urea and on arginindihydrolase.]". Biochim. Biophys. Acta. 6 (1): 210–6. PMID 14791411. 
• Yoshimoto T, Oka I, Tsuru D (Tokyo). "Purification, crystallization, and some properties of creatine amidinohydrolase from Pseudomonas putida". J. Biochem. (6): 1381–3. PMID 8443. 

This hydrolase article is a stub. You can help Wikipedia by expanding it.v · Categories: Hydrolase stubs EC 3.5.3 Enzymes of known structure Wikimedia Foundation. 2010. Игры ⚽ Нужна курсовая? Nobuhiro Kiyotaki Garden Island (Ontario) Look at other dictionaries: creatinase — An enzyme catalyzing the hydrolysis of creatine to sarcosine and urea. * * * (creatine kinase) n. an enzyme involved in the metabolic breakdown of creatine to creatinine. * * * cre·at·i·nase (kre atґĭ nās) [EC 3.5.3.3] an amidohydrolase …   Medical dictionary creatinase — creatine kinase; n. an enzyme involved in the metabolic breakdown of creatine to creatinine …   The new mediacal dictionary Креатиназа (Creatinase), Креатинкиназа (Creatine Kinase) — фермент, участвующий в метаболическом разложении креатина до креатинина. Источник: Медицинский словарь …   Медицинские термины creatine kinase — creatinase …   The new mediacal dictionary List of EC numbers (EC 3) — This list contains a list of EC numbers for the third group, EC 3, hydrolases, placed in numerical order as determined by the Nomenclature Committee of the International Union of Biochemistry and Molecular Biology.EC 3.1: Acting on Ester BondsEC… …   Wikipedia Creatininase — Crystallographic structure of a creatinine amidohydrolase from Nostoc pruniforme.[1] Identifiers EC number …   Wikipedia Hydrolase — Cristal de lysozyme Les hydrolases constituent une classe d enzymes qui catalysent les réactions d hydrolyse de molécules suivant la réaction générale : R R + H2O ⇌ R OH + R H On y trouve par exemple les estérases, qui hydrolysent les …   Wikipédia en Français amidinohydrolases — Enzymes cleaving linear amidines; e.g., arginase, creatinase …   Medical dictionary EC 3.5 — Le groupe EC3.5 rassemble des enzymes appartenant à la famille des hydrolases. Elles ont pour particularité de catalyser des réactions d hydrolyse dans des molécules possédant un carbone et un azote liés par liaison simple, double ou triple. À la …   Wikipédia en Français 18+ © Academic, 2000-2024 Contact us: Technical Support, Advertising Dictionaries export, created on PHP, Joomla, Drupal, WordPress, MODx. Mark and share Search through all dictionaries Translate… Search Internet Share the article and excerpts Direct link … Do a right-click on the link above and select “Copy Link”