URA3


URA3

URA3 encodes orotidine 5-phosphate decarboxylase (ODCase), an enzyme involved in the synthesis of pyrimidine ribonucleotides. Loss of ODCase activity leads to a lack of cell growth unless uracil or uridine is added to the media (positive selection). In contrast, if 5-FOA is added to the media ODCase can convert into the toxic compound 5-fluorouracil causing death (negative selection).

Since URA3 allows for both positive and negative selection, it has been developed as a genetic marker for DNA transformations and other genetic techniques in bacteria and many fungal species. It is one of the most important genetic markers in yeast genetic modification.

References

S. cerevisiae database (2005) URA3 gene. http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ura3#summaryParagraph (accessed 16/10/07).

PAUL J. FLYNN AND RICHARD J. REECE. Activation of Transcription by Metabolic Intermediates of the Pyrimidine Biosynthetic Pathway. MOLECULAR AND CELLULAR BIOLOGY,Vol. 19, No. 1, 882–888


Wikimedia Foundation. 2010.

Look at other dictionaries:

  • Two-hybrid screening — Overview of two hybrid assay, checking for interactions between two proteins, called here Bait and Prey . A. Gal4 transcription factor gene produces two domain protein ( BD and AD ) which is essential for transcription of the reporter gene ( LacZ …   Wikipedia

  • Orotidine 5'-phosphate decarboxylase — E. coli OMP decarboxylase.[1] Identifiers EC number …   Wikipedia

  • Pyrimidin-De-Novo-Synthese — Unter dem Begriff Pyrimidin De Novo Synthese versteht man die biochemische Herstellung von Pyrimidin Nukleotiden aus einfacheren Molekülen. Im Gegensatz zum Salvage Pathway werden hier keine Nukleotide oder Nukleotidderivate umgebaut und… …   Deutsch Wikipedia

  • Pyrimidin de-novo Synthese — Unter dem Begriff Pyrimidin De Novo Synthese versteht man die biochemische Herstellung von Pyrimidin Nukleotiden aus einfacheren Molekülen. Im Gegensatz zum Salvage Pathway werden hier keine Nukleotide oder Nukleotidderivate umgebaut und… …   Deutsch Wikipedia

  • Pyrimidin-de novo-Synthese — Übergeordnet Nukleosidsynthese Nukleotidsynthese Untergeordnet UMP Synthese CMP Synthese TMP Synthese …   Deutsch Wikipedia

  • Plasmid — Figure 1: Illustration of a bacterium with plasmid enclosed showing chromosomal DNA and plasmids. In microbiology and genetics, a plasmid is a DNA molecule that is separate from, and can replicate independently of, the chromosomal DNA …   Wikipedia

  • FOA — The three letter acronym FOA has multiple meanings, including: * Full Operational Assessment (in air traffic control) * Field Operating Agency (in military usage) * Freedom of Access (in politics) * Funding Opportunity Announcement (for example,… …   Wikipedia

  • Shuttle vector — A shuttle vector is a vector (usually a plasmid) constructed so that it can propagate in two different host species [http://www.ncbi.nlm.nih.gov/sites/entrez?db=Books cmd=Search term=vector%20AND%20mcb%5Bbook%5D doptcmdl=TOCView] ). Therefore,… …   Wikipedia

  • Cromosoma artificial de levadura — El cromosoma artificial de levaduras o YAC (Yeast artificial chromosome) forma parte de los vectores de clonación de alta capacidad siendo, de hecho, el de mayor capacidad (200 kb 3.000 kb). Fueron descritos por primera vez en 1983 por Murray y… …   Wikipedia Español

  • uro — URO1 , UREO , URÉZĂ, URÍE, URINI , elem. Urină , uree, acid uric . (din fr. uro , uréo , urèse, urie, urini ; cf. gr. ouron, ouresis) Trimis de tavi, 20.04.2005. Sursa: MDN  URO2 , ÚRĂ3, elem. Coadă . (din fr. uro , oure; cf. gr. oura) …   Dicționar Român