Intact protein expression spectrometry


Intact protein expression spectrometry

Intact protein expression spectrometry (IPEx) is a label-free quantitation approach in mass spectrometry under development by the analytical chemistry group at the United States Food and Drug Administration Center for Food Safety and Applied Nutrition and elsewhere. Intact proteins are analyzed by an LCMS instrument, usually a quadrupole time-of-flight in profile mode, and the full protein profile is determined and quantified using data reduction software. Early results are very encouraging. In one study, two groups of treatment replicates from mammalian samples (different organisms with similar treatment histories, but not technical replicates) show dozens of low CV protein biomarkers, suggesting that IPEx is a viable technology for studying protein expression. [Scholl, PF, "An intact protein LC/MS strategy for serum biomarker development: Biomarkers of hepatic responsiveness to chemopreventive treatment with the triterpenoid CDDO-Im", [http://asms.org/Default.aspx?tabid=54&type=viewabstract&lognumber=1034 Abstract] , TOA 8:35 a.m. ASMS Conference, 2007.]

See also

* Mass spectrometry software

References


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