RNA-OUT

RNA-OUT

RNA-OUT is a non-coding RNA that is antisense to the RNA-IN non-coding RNA. Transposition of insertion sequence IS10 is regulated by an anti-sense RNA which inhibits transposase expression when IS10 is present in multiple copies per cell. RNA-OUT consists of a stem-loop domain topped by a flexibly paired loop; the 5' end of the target molecule, RNA-IN, is complementary to the top of the loop, and complementarity extends for 35 nucleotides down one side of RNA-OUT. [cite journal | last = Kittle | first = JD | coauthors = Simons RW, Lee J, Kleckner N | year = 1989 | title = Insertion sequence IS10 anti-sense pairing initiates by an interaction between the 5' end of the target RNA and a loop in the anti-sense RNA | journal = J Mol Biol | volume = 210 | pages = 561–572 | pmid = 2482367 | doi = 10.1016/0022-2836(89)90132-0]

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