Auramine-rhodamine stain


Auramine-rhodamine stain

The auramine-rhodamine stain (AR), also known as the "Truant auramine-rhodamine stain", is a histological technique used to visualizee acid-fast bacilli using fluorescence microscopy, notably species in the "Mycobacterium" genus.cite journal |author=Kommareddi S, Abramowsky C, Swinehart G, Hrabak L |title=Nontuberculous mycobacterial infections: comparison of the fluorescent auramine-O and Ziehl-Neelsen techniques in tissue diagnosis |journal=Hum Pathol |volume=15 |issue=11 |pages=1085–9 |year=1984 |pmid=6208117 |doi=10.1016/S0046-8177(84)80253-1] Acid-fast organisms display a reddish-yellow fluorescence.cite journal |author=Abe C |title= [Standardization of laboratory tests for tuberculosis and their proficiency testing] |journal=Kekkaku |volume=78 |issue=8 |pages=541–51 |year=2003 |pmid=14509226]

AR stain is a mixture of Auramine O and Rhodamine B.

See also

* Auramine O
* Biological stains
* Ziehl-Neelsen stain

References


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  • Truant auramine-rhodamine stain — a method for demonstrating mycobacteria. A smear is heat fixed, stained with auramine rhodamine solution, decolorized, counterstained with potassium permanganate, and examined under ultraviolet light; acid fast organisms glow with a yellow orange …   Medical dictionary

  • Rhodamine — (IPAEng|ˈroʊdəmiːn) is a family of related chemical compounds, fluorone dyes. Examples are Rhodamine 6G and Rhodamine B. They are used as a dye and as a dye laser gain medium. It is often used as a tracer dye within water to determine the rate… …   Wikipedia

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