HIS3

HIS3

The HIS3 gene, found in the "Saccharomyces cerevisiae" yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis. [ [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3 HIS3/YOR202W Summary ] ] It is analogous to hisB in "Escherichia coli".

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Mutations in "Escherichia coli"'s analogous gene, hisB allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of "E. coli" in which a desired protein-DNA or protein-protein interaction is taking place (Joung et al., 2000)

References

*Joung, J. K., E. I. Ramm and C. O. Pabo (2000). "A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions." Proc Natl Acad Sci U S A 97(13): 7382-7.


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