- Malaria culture
Malaria culture is the method to grow malaria parasites outside the body i.e. in an ex vivo environment. Plasmodium falciparum is currently the only human malaria parasite that has been successfully cultured continuously ex vivo. Although attempts for propagation of the parasites outside of humans or animal models reach as far back as 1912, the success of the initial attempts was limited to one or just a few cycles. The first successful continuous culture was established in 1976. Initial hopes that the ex vivo culture would lead quickly to the discovery of a vaccine were premature. However, the development of new drugs was greatly facilitated.
Infected human red blood cells are incubated in a culture dish or flask at 37°C together with a nutrient medium and plasma, serum or serum substitutes. A special feature of the incubation is the special gas mixture of mostly nitrogen (93% nitrogen, 4% carbondioxide, 3% oxygen) allowing the parasites to grow at 37°C in a cell incubator. An alternative to gasing the cultures with the exact gas mixture, is the use of a candlejar. The candlejar is an airtight container in which the cultures and a lit candle are placed. The burning candle consumes some of the oxygen and produces carbon dioxide (CO2), which acts as a fire extinguisher. Carbon dioxide content in fresh air varies between 0.036% and 0.039%, at an app. 5% CO2 concentration the candle stops burning. The number of parasites increased by a factor 5 approximately every 48 hours (= one cycle). The parasitemia can be determined via blood film, to keep it within the wanted limits, the culture can be thinned out with healthy red blood cells.
The original method for the successful ex vivo propagation of P. falciparum described culture of the parasite under static conditions (Trager-Jensen method). James B. Jensen joined Trager’s laboratory as a post-doctoral fellow in 1976. He decided to employ a candlejar instead of the CO2 incubator. In the summer of 1976 Milton Friedman, a graduate student in the Trager lab who was working in the MRC laboratories in The Gambia, arranged for a sample of human blood infected with P. falciparum to be sent to New York. This was diluted with RPMI 1640 (which turned out to be the best of the commercial media) in Petri dishes, placed in a candlejar and incubated. The line grew very well and became FCR-3/Gambia, one of the most widely used strains. Later, other lines would be established using similar methods and the impact of continuous cultivation of P. falciparum was phenomenal especially for the testing of putative antimalarials and for deciphering its genes. A number of subsequent reports (from as far back as the early 1980s), showed that cell suspension (using a shaking-incubator) significantly increased culture growth. Continuous agitation has also been shown to improve other parameters of culture growth relevant to researchers, such as the prolongation of culture synchrony after synchronization procedures, and a reduction of the rate of multiple infections. Despite this, the practice of culturing the parasite under static conditions remains widespread. The greatest value of the candlejar method is that it can be used in laboratories almost anywhere in the world where there is an incubator, a candle and a desiccator. Around 60% parasitized cells can be obtained using optimized culturing conditions. Recent studies of P. falciparum isolated directly from infected patients indicate that alternative parasite biological states occur in the natural host that are not observed with ex vivo cultivated parasites.
Concentration of infected cells
infected red blood cells because red cells containing plasmodia are less dense than normal ones. Young trophozoites coincided with erythrocytes in a broad band corresponding to densities from 1.075 to 1.100 g/ml, whereas schizonts were concentrated at a density approximating 1.062 g/ml. There are studies, however, that suggest that some strains of P.falciparum are affected in their capacity of invasion after being exposed to this chemical. The difference between diamagnetic low-spin oxyhemoglobin in uninfected red blood cells and paramagnetic hemozoin in infected red blood cells can also be used for isolation. Magnetic columns have shown to be less harmful for the parasite and are simple and adjustable to the needs of the researcher. The column is mounted in a potent magnet holder and the culture flowed through it. The column traps the erythrocytes infected with the latest stages of the parasites, which can then be eluted when the column is removed from the magnet. It is a simple method that does not need expensive equipment and it does not seem to affect the parasites as to their invasion capabilities afterwards.
- ^ a b c Radfar A, Méndez D, Moneriz C, Linares M, Marín-García P, Puyet A, Diez A, Bautista JM (2009). "Synchronous culture of Plasmodium falciparum at high parasitemia levels". Nat Protoc 4 (11): 1828–1644. doi:10.1038/nprot.2009.198. PMID 20010926.
- ^ Bass CC, Johns FM. (1912). "The Cultivation of Malarial Plasmodia (Plasmodium vivax and Plasmodium Falciparum) in vitro". J. Exp. Med. 16 (4): 567–579. doi:10.1084/jem.16.4.567. PMC 2124976. PMID 19867597. http://jem.rupress.org/cgi/reprint/16/4/567.
- ^ a b Trager W, Jensen JB (1976). "Human malaria parasites in continuous culture". Science 193 (4254): 673–675. doi:10.1126/science.781840. PMID 781840.
- ^ Trager W, Jensen JB (1997). "Continuous culture of Plasmodium falciparum: its impact on malaria research". Int J Parasitol 27 (9): 989–1006. doi:10.1016/S0020-7519(97)00080-5. PMID 9363481.
- ^ Basco LK (2003). "Molecular epidemiology of malaria in Cameroon. XV. Experimental studies on serum substitutes and supplements and alternative culture media for in vitro drug sensitivity assays using fresh isolates of Plasmodium falciparum". Am J Trop Med Hyg 69 (2): 168–173. PMID 14506772. http://www.ajtmh.org/cgi/content/full/69/2/168.
- ^ Trigg PI (1985). "Recent advances in malaria parasite cultivation and their application to studies on host-parasite relationships: a review". Bull World Health Organ 63 (2): 387–398. PMC 2536397. PMID 3893779. http://whqlibdoc.who.int/bulletin/1985/Vol63-No2/bulletin_1985_63%282%29_387-398.pdf.
- ^ Schuster FL (2002). "Cultivation of plasmodium spp". Clin Microbiol Rev 15 (3): 355–364. doi:10.1128/CMR.15.3.355-364.2002. PMC 118084. PMID 12097244. http://cmr.asm.org/cgi/reprint/15/3/355.
- ^ Rüssmann L, Jung A, Heidrich HG (1982). "The use of percoll gradients, elutriator rotor elution, and mithramycin staining for the isolation and identification of intraerythrocytic stages of plasmodium berghei". Z Parasitenkd 66 (3): 273–280. doi:10.1007/BF00925344. PMID 6177116.
- ^ Allen RJW, Kirk K (2010). "Plasmodium falciparum culture: The benefits of shaking". Mol Biochem Parasitol 169 (1): 65–63. doi:10.1016/j.molbiopara.2009.09.005. PMID 19766147.
- ^ Sherman IW (2010). "Magic Bullets to Conquer Malaria. From Quinine to Qinghaosu". Asm Press, ISBN 978-1555815431.
- ^ LeRoux M, Lakshmanan V, Daily JP (2009). "Plasmodium falciparum biology: analysis of in vitro versus in vivo growth conditions". Trends Parasitol 25 (10): 474–481. doi:10.1016/j.pt.2009.07.005. PMID 19747879. http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6W7G-4X6C0VX-2&_user=10&_coverDate=10%2F31%2F2009&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_searchStrId=1413244790&_rerunOrigin=google&_acct=C000050221&_version=1&_urlVersion=0&_userid=10&md5=f7161c781abbc262325083df5fb5d9a3.
- ^ a b Spadafora C, Gerena L, Kopydlowski KM (2011). "Comparison of the in vitro invasive capabilities of Plasmodium falciparum schizonts isolated by Percoll gradient or using magnetic based separation". Malar J 10 (1): 96. doi:10.1186/1475-2875-10-96. PMC 3100259. PMID 21501476. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=3100259.
- ^ Rivadeneira EM, Wasserman M, Espinal CT (1983). "Separation and concentration of schizonts of Plasmodium falciparum by Percoll gradients". J Protozool 30 (2): 367–370. doi:10.1111/j.1550-7408.1983.tb02932.x. PMID 6313915. http://www3.interscience.wiley.com/journal/119550066/abstract?CRETRY=1&SRETRY=0.
- ^ Kim CC, Wilson EB, Derisi JL (2010). "Improved methods for magnetic purification of malaria parasites and haemozoin". Malar J 9 (1): 17. doi:10.1186/1475-2875-9-17. PMC 2817699. PMID 20074366. http://www.malariajournal.com/content/pdf/1475-2875-9-17.pdf.
- ^ Bhakdi SC, Ottinger A, Somsri S, Sratogno P, Pannadaporn P, Chimma P, Malasit P, Pattanapanyasat K, Neumann HP (2010). "Optimized high gradient magnetic separation for isolation of Plasmodium-infected red blood cells". Malar J 9 (1): 38. doi:10.1186/1475-2875-9-38. PMC 2831011. PMID 20122252. http://www.malariajournal.com/content/pdf/1475-2875-9-38.pdf.
Wikimedia Foundation. 2010.
Look at other dictionaries:
Malaria — Classification and external resources Ring forms and gametocytes of Plasmodium falciparum in human blood. ICD 10 B … Wikipedia
Malaria — Paludisme Classifications CIM du Paludisme Classification et ressources externes CIM 10 B50 à B54 CIM 9 084 (84.0 à 84.9) … Wikipédia en Français
History of malaria — The history of malaria predates humanity, as this ancient disease evolved before humans did. Malaria, a widespread and potentially lethal infectious disease, has afflicted people for much of human history, and has affected settlement patterns.… … Wikipedia
primitive culture — Introduction in the lexicon of early anthropologists, any of numerous societies characterized by features that may include lack of a written language, relative isolation, small population, relatively simple social institutions and… … Universalium
Melanesian culture — ▪ cultural region, Pacific Ocean Introduction the beliefs and practices of the indigenous peoples of the ethnogeographic group of Pacific Islands known as Melanesia. From northwest to southeast, the islands form an arc that begins with New… … Universalium
Society and culture of the Han Dynasty — A Western Han jade carved door knocker with designs of Chinese dragons (and two other jade figurines) The Han Dynasty (206 BCE – 220 CE) was a period of ancient China divided by the Western Han (206 BCE – 9 CE) and Eastern Han (25–220 CE) periods … Wikipedia
Paludisme — Classifications CIM du Paludisme Classification et ressources externes Frottis sanguin révélant la présence du parasite Plasmodium falciparum ayant la forme d anneaux à l intérieur d hématies humaines … Wikipédia en Français
Plasmodium — This article is about the malaria parasite. For plasmodial slime molds, see Slime mold. Plasmodium Scientific classification Domain: Eukaryota … Wikipedia
Antipaludique — Paludisme Classifications CIM du Paludisme Classification et ressources externes CIM 10 B50 à B54 CIM 9 084 (84.0 à 84.9) … Wikipédia en Français
Antipaludéen — Paludisme Classifications CIM du Paludisme Classification et ressources externes CIM 10 B50 à B54 CIM 9 084 (84.0 à 84.9) … Wikipédia en Français