Aniline acetate test

Aniline acetate test

The aniline acetate test is a chemical test to identify the presence of certain carbohydrates. When any of these carbohydrates reacts with aniline acetate, furfural is produced, causing a bright pink color.

Hydrochloric acid interacts with pentoses (sugars containing five carbon atoms) to produce furfural. This causes a bright pink color. This distinguishes pentoses (sugars containing five carbons) and hexoses (sugars containing six carbons). 3-Furanaldehyde responds to the usual aldehyde tests, but unlike 2-furanaldehyde it gives no color test with aniline acetate.

References

* "A Method for the identification of pure organic compounds by a systematic analytical procedure based on physical properties and chemical reactions." v. 1, 1911. By Samuel Parsons Mulliken. Published by J. Wiley & Sons, Inc., 1904. [http://books.google.com/books?id=ay-imBeBfmUC&pg=PA33&lpg=PA33&dq=aniline+acetate+test&source=web&ots=OmZi9QADq8&sig=dQZF13mKpS58BP7cuL5MIRufG98&hl=en&sa=X&oi=book_result&resnum=6&ct=result Google Books link] page 33.
* "Modifications of the aniline acetate-furfural method for the determination of pentose." The Analyst, 1956, 81, 598 - 601, DOI: 10.1039/AN9568100598 [http://www.rsc.org/publishing/journals/AN/article.asp?doi=AN9568100598]


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