Reverse transcription


Reverse transcription

Reverse transcription is the process of making a double stranded DNA (deoxyribonucleic acid) molecule from a single stranded RNA (ribonucleic acid) template. It is called reverse transcription as it acts in the opposite or reverse direction to transcription. This idea was very unpopular at first as it contradicted the Central Dogma of Molecular Biology which states DNA is transcribed into RNA which is translated into proteins. However, in 1970 when the scientists Howard Temin and David Baltimore both independently discovered the enzyme responsible for reverse transcription, named reverse transcriptase, the possibility that genetic information could be passed on in this manner was finally accepted.

Reverse transcription in Class VI viruses

Class VI viruses ssRNA-RT, also called the retroviruses are RNA reverse transcribing viruses with a DNA intermediate. Their genomes consist of two molecules of positive sense single stranded RNA with a 5' cap and 3' polyadenylated tail. Examples of retroviruses include "Human Immunodeficiency Virus" (HIV) and "Human T-Lymphotropic virus" (HTLV). Once the viruses have entered the cell and been uncoated the genome is reverse transcribed into double stranded DNA which can be incorporated into the host cell and subsequently expressed.Reverse transcription by the enzyme reverse transcriptase occurs in a series of steps:
#A specific cellular tRNA acts as a primer and hybridizes to a complementary part of the virus genome called the primer binding site or PBS
#Complementary DNA then binds to the U5 (non-coding region) and R region (a direct repeat found at both ends of the RNA molecule) of the viral RNA
#A domain on the reverse transcriptase enzyme called RNAse H degrades the 5’ end of the RNA which removes the U5 and R region
#The primer then ‘jumps’ to the 3’ end of the viral genome and the newly synthesised DNA strands hybridizes to the complementary R region on the RNA
#The first strand of complementary DNA (cDNA) is extended and the majority of viral RNA is degraded by RNAse H
#Once the strand is completed, second strand synthesis is initiated from the viral RNA
#There is then another ‘jump’ where the PBS from the second strand hybridizes with the complementary PBS on the first strand
#Both strands are extended further and can be incorporated into the hosts genome by the enzyme integrase

References

*Alan J. Cann, Principles of Molecular Virology (1997) Second Edition, Academic Press, ISBN 0-12-158532-8

* [http://www.microbiologybytes.com/virology/Retroviruses.html MicrobiologyBytes:Retroviruses]


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Look at other dictionaries:

  • reverse transcription — n the process of synthesizing DNA using RNA as a template and reverse transcriptase as a catalyst reverse transcribe vt, scribed; scrib·ing * * * the synthesis of a DNA molecule complementary to RNA molecule, the RNA acting as a template;… …   Medical dictionary

  • reverse transcription — reverse transcription. См. обратная транскрипция. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • reverse transcription — atvirkštinė transkripcija statusas T sritis augalininkystė apibrėžtis Genetinės informacijos apie RNR sudėtį perrašymas į DNR. atitikmenys: angl. reverse transcription rus. обратная транскрипция ryšiai: sinonimas – atvirkštinis nurašymas …   Žemės ūkio augalų selekcijos ir sėklininkystės terminų žodynas

  • Reverse transcription — Transcription (biologie) Pour les articles homonymes, voir Transcription. Transcription/Traduction La transcription est un processus biolog …   Wikipédia en Français

  • reverse transcription — noun : the process of synthesizing DNA using RNA as a template and reverse transcriptase as a catalyst compare transcription herein …   Useful english dictionary

  • reverse transcription — noun Date: 1971 the process of synthesizing DNA using RNA as a template and reverse transcriptase as a catalyst …   New Collegiate Dictionary

  • reverse transcription — noun The process of creating double stranded DNA from a single stranded RNA template (the reverse of the standard process) …   Wiktionary

  • reverse transcription — The synthesis of DNA from a template of RNA, accomplished by reverse transcriptase …   Glossary of Biotechnology

  • reverse transcription-polymerase chain reaction — reverse transcription polymerase chain reaction. См. полимеразная цепная реакция с обратной транскриптазой. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Reverse transcription polymerase chain reaction — In molecular biology, reverse transcription polymerase chain reaction (RT PCR) is a laboratory technique for amplifying a defined piece of a ribonucleic acid (RNA) molecule. The RNA strand is first reverse transcribed into its DNA complement or… …   Wikipedia


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