Artificial transcription factor
- Artificial transcription factor
An artificial transcription factor is an example of a chimeral protein, designed to target and modulate gene transcription. [cite journal |author=Gommans W, Haisma H, Rots M |title=Engineering zinc finger protein transcription factors: the therapeutic relevance of switching endogenous gene expression on or off at command |journal=J. Mol. Biol. |volume=354 |issue=3 |pages=507–19 |year=2005 |pmid=16253273 |url=http://www.rug.nl/farmacie/onderzoek/basiseenheden/therapeuticgenemodulation/publicaties/publicaties2004/2005_6.pdf]
They are generally composed of a DNA-binding domain (specific to a certain sequence) coupled to a modulatory domain (which acts upon other transcription factors) in order to alter the expression of a particular gene. It is also possible to downregulate expression of a gene by targeting the 5' untranslated region with a DNA-binding domain that lacks a regulatory domain; this will reduce transcription simply by blocking RNA polymerase progression along the DNA template.
See also
*Therapeutic gene modulation
*Zinc finger
*Zinc finger protein transcription factor
*Chimera (protein)
*Protein engineering
References
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2010.
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