- Golgi's method
Golgi's method is a
nervous tissue stainingtechnique discovered by Italian physicianand scientist Camillo Golgi(1843-1926) in 1873. It was initially named the black reaction ("la reazione nera") by Golgi, but it became better known as the Golgi stain or method later.
Golgi' staining was famously used by Spanish
neuroanatomist Santiago Ramón y Cajal(1852-1934) to discover a number of novel facts about the organization of the nervous system, inspiring the birth of the neuron doctrine.
The cells in nervous tissue are densely packed and little information on their structures and interconnections can be obtained if all the cells are stained. Furthermore, its thin filamentary extensions—the
axonand the dendrites—are too slender and transparent to be seen with normal staining techniques. Golgi's method stains a limited number of cells at random in their entirety. The mechanism by which this happens is still largely unknown [cite book
title=From neuron to brain
pages=5 ] . Dendrites, as well as the cell soma, are clearly stained in brown and black and can be followed in their entire length, which allowed neuroanatomists to track connections between neurons and to make visible the complex networking structure of many parts of the
brainand spinal cord.
Golgi's staining is achieved by impregnating fixed nervous tissue with
potassium dichromateand silver nitrate. Cells thus stained are filled by microcrystallization of silver chromate.
According to SynapseWeb [http://synapses.mcg.edu/learn/visualize/visualize.stm] , this is the recipe for Golgi's staining technique:
#Immerse a block (approx. 10x5 mm) of formol-fixed (or
paraformaldehyde- glutaraldehyde-perfused) brain tissue into a 2% aqueous solution of potassium dichromatefor 2 days
#Dry the block shortly with
#Immerse the block into a 2% aqueous solution of
silver nitratefor another 2 days.
#Cut sections approx. 20-100 µm thick.
#Dehydrate quickly in
ethanol, clear and mount (e.g., into Depexor Enthalan).
This technique has since been refined to substitute the silver precipitate with gold by immersing the sample in gold chloride then oxalic acid, followed by removal of the silver by sodium thiosulphate. This preserves a greater degree of fine structure with the ultrastructural details marked by small particles of gold. [http://www.springerlink.com/(f0cb2ybwsglgtnuip5efub55)/app/home/contribution.asp?referrer=parent&backto=issue,5,11;journal,215,243;linkingpublicationresults,1:100182,1]
Cajal said of the Golgi method:
:" I expressed the surprise which I experienced upon seeing with my own eyes the wonderful revelatory powers of the chrome-silver reaction and the absence of any excitement in the scientific world aroused by its discovery." : "Recuerdos de mi vida, Vol. 2, Historia de mi labor científica". Madrid: Moya, 1917, p. 76.
* [http://www.ihcworld.com/imagegallery/displayimage.php?album=4&pos=16 Photomicrograph of a cortex cell stained with Golgi's] . IHC Image Gallery.
* [http://flybrain.neurobio.arizona.edu/Flybrain/html/atlas/golgi/ Golgi impregnations] . Images of the brain of flies.
* [http://synapses.mcg.edu/learn/visualize/visualize.stm Visualization of dendritic spines using Golgi Method] . SynapseWeb. Includes a time-lapse study of Golgi impregnation.
* Berrebi, Albert: [http://www.ihcworld.com/imagegallery/displayimage.php?album=4&pos=13 Cell Biology of Neurons: Structure and Methods of Study] . (in PDF)
* Valverde, Facundo. [http://www.cajal.csic.es/Valverde%20Web%2002/Golgi%20atlas.htm Golgi Atlas of the Post Natal Mouse Brain] .
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См. также в других словарях:
golgi method — noun Usage: usually capitalized G Etymology: after C. Golgi : a method of preparing tissues for the study of nerves by using potassium bichromate and silver nitrate … Useful english dictionary
Golgi method — n a method of preparing nerve tissue for study using potassium dichromate and silver nitrate that is effective because a few of the neurons are stained completely and stand out from the rest which are not stained at all … Medical dictionary
Golgi — Camillo, Italian histologist and Nobel laureate, 1843–1926. See G. apparatus, G. complex, G. corpuscle, G. tendon organ, G. internal reticulum, G. zone, G. cells, under cell, G. osmiobichromate fixative, G. stain, G. Mazzoni corpuscle, Holmgrén G … Medical dictionary
Golgi, Camillo — born July 7, 1843/44, Corteno, Italy died Jan. 21, 1926, Pavia Italian physician and cytologist. He devised a way to stain nerve tissue and with it discovered a neuron, now called the Golgi cell, that has many short, branching extensions… … Universalium
Golgi , Camillo — (1843–1926) Italian cytologist and histologist Born at Corteno near Brescia (now in Italy), Golgi studied medicine at Pavia University and thereafter mainly concerned himself with research on cells and tissues. In 1873, while serving as physician … Scientists
Golgi — I. adjective Date: 1891 of or relating to the Golgi apparatus, Golgi bodies, or a method of staining nerve tissue < Golgi vesicles > II. biographical name Camillo 1843(or 1844) 1926 Italian physician … New Collegiate Dictionary
Camillo Golgi — Infobox Scientist name = Camillo Golgi image width = 150px caption = Camillo Golgi, 1906 birth date = birth date|1843|7|7|mf=y birth place = Corteno, Italy death date = death date and age|1926|01|21|1843|7|7 death place = Pavia, Italy residence … Wikipedia
Scientific method — … Wikipedia
Neuron — This article is about cells in the nervous system. For other uses, see Neuron (disambiguation). Brain cell redirects here. For other uses, see Glial cell. Neuron: Nerve Cell … Wikipedia
Neuromorphology — (from Greek νεῦρον, neuron, nerve ; μορφή, morphé, “form”; λογία, logia, “study of”) is the study of nervous system form, shape, and structure. The study of its structure includes looking at the organ system from a physiological and… … Wikipedia