Gene delivery


Gene delivery

Gene delivery is the process of introducing foreign DNA into host cells. Gene delivery is, for example, one of the steps necessary for gene therapy and the genetic modification of crops. There are many different methods of gene delivery developed for a various types of cells and tissues, from bacteria to mammalian. Generally, the methods can be divided into two categories, viral and non-viral.

Virus mediated gene delivery utilizes ability of a virus to inject its DNA inside a host cell. A gene that is intended for delivery is packaged into a viral particle.

Non-viral methods include physical methods such as microinjection, gene gun, impalefection, hydrostatic pressure, electroporation, continuous infusion, and sonication and chemical, such as lipofection. It can also include the use of polymeric gene carriers (polyplexes) [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17675152]

References

*Segura, T. and L.D. Shea, Materials for non-viral gene delivery. Annual Review of Materials Research, 2001. 31: p. 25-46.

*Luo, D. & Saltzman, W. M. Synthetic DNA delivery systems. Nature Biotechnol. 18,33–37 (2000).

*Saul JM, Linnes MP, Ratner BD, Giachelli CM, Pun SH. Delivery of non-viral gene carriers from sphere-templated fibrin scaffolds for sustained transgene expression. Biomaterials 2007;28(31):4705-16. [http://www.ncbi.nlm.nih.gov/sites/entrez?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=17675152]

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