- Mosaic (genetics)
In genetic medicine, a mosaic or mosaicism denotes the presence of two populations of cells with different genotypes in one individual who has developed from a single fertilized egg. Mosaicism may result from a mutation during development which is propagated to only a subset of the adult cells.
Different types of mosaicism exist, such as gonadal mosaicism (restricted to the gametes) or tissue or somatic mosaicism.
Somatic mosaicism occurs when the somatic cells of the body are of more than one genotype. In the more common mosaics, different genotypes arise from a single fertilized egg cell, due to mitotic errors at first or later cleavages.
The most common form of mosaicism found through prenatal diagnosis involves trisomies. Although most forms of trisomy are due to problems in meiosis and affect all cells of the organism, there are cases where the trisomy occurs in only a selection of the cells. This may be caused by a nondisjunction event in an early mitosis, resulting in a loss of a chromosome from some trisomic cells. Generally this leads to a milder phenotype than in non-mosaic patients with the same disorder.
An example of this is one of the milder forms of Klinefelter's syndrome, called 46/47 XY/XXY mosaic wherein some of the patient's cells contain XY chromosomes, and some contain XXY chromosomes. The 46/47 annotation indicates that the XY cells have the normal number of 46 total chromosomes, and the XXY cells have 47 total chromosomes.
Around 30% of Turner's syndrome cases demonstrate mosaicism, while complete monosomy (45 XO) occurs in about 50–60% of cases.
True mosaicism should not be mistaken for the phenomenon of X-inactivation, where all cells in an organism have the same genotype, but a different copy of the X chromosome is expressed in different cells, such as in calico cats.
One basic mechanism which can produce mosaic tissue is mitotic recombination or somatic crossing-over. It was first discovered by Curt Stern in Drosophila in 1936. The process involves exchange of genetic material between chromatids of homologous chromosomes. It occurs much less often than meiotic recombination. The amount of tissue which is mosaic depends on where, in the tree of cell division, the exchange takes place.
The cause is usually a mutation that occurred in an early stem cell that gave rise to all or part of the gonadal tissue.
This can cause only some children to be affected, even for a dominant disease.
Use in experimental biology
Genetic mosaics can be extraordinarily useful in the study of biological systems, and can be created intentionally in many model organisms in a variety of ways. They often allow for the study of genes that are important for very early events in development, making it otherwise difficult to obtain adult organisms in which later effects would be apparent. Furthermore they can be used to determine the tissue or cell type in which a given gene is required and to determine whether a gene is cell autonomous. That is, whether or not the gene acts solely within the cell of that genotype, or if it affects neighboring cells which do not themselves contain that genotype, but take on that phenotype due to environmental differentiation.
The earliest examples of this involved transplantation experiments (technically creating chimeras) where cells from a blastula stage embryo from one genetic background are aspirated out and injected into a blastula stage embryo of a different genetic background.
Genetic mosaics are a particularly powerful tool when used in the commonly studied fruit fly, where they are created through mitotic recombination. Mosaics were originally created by irradiating flies heterozygous for a particular allele with X-rays, inducing double-strand DNA breaks which, when repaired, could result in a cell homozygous for one of the two alleles. After further rounds of replication, this cell would result in a patch, or "clone" of cells mutant for the allele being studied.
More recently the use of a transgene incorporated into the Drosophila genome has made the system far more flexible. The Flip Recombinase (or FLP) is a gene from the commonly studied yeast Saccharomyces cerevisiae which recognizes "Flip Recombinase Target" sites, which are short sequences of DNA, and induces recombination between them. FRT sites have been inserted transgenically near the centromere of each chromosome arm of Drosophila melanogaster. The FLP gene can then be induced selectively, commonly using either the heat shock promoter or the GAL4/UAS system. The resulting clones can be identified either negatively or positively.
In negatively marked clones the fly is transheterozygous for a gene encoding a visible marker (commonly the green fluorescent protein, GFP) and an allele of a gene to be studied (both on chromosomes bearing FRT sites). After induction of FLP expression, cells that undergo recombination will have progeny that are homozygous for either the marker or the allele being studied. Therefore the cells that do not carry the marker (which are dark) can be identified as carrying a mutation.
It is sometimes inconvenient to use negatively marked clones, especially when generating very small patches of cells, where it is more difficult to see a dark spot on a bright background than a bright spot on a dark background. It is possible to create positively marked clones using the so called MARCM ("Mosaic Analysis with a Repressible Cell Marker", pronounced mark-em) system, developed by Liqun Luo, a professor at Stanford University and his post-doc, Tzumin Lee, now a group leader at Janelia Farm. This system builds on the GAL4/UAS system, which is used to express GFP in specific cells. However a globally expressed GAL80 gene is used to repress the action of GAL4, preventing the expression of GFP. Instead of using GFP to mark the wild type chromosome as above, GAL80 serves this purpose, so that when it is removed by mitotic recombination, GAL4 is allowed to function, and GFP turns on. This results in the cells of interest being marked brightly in a dark background.
- ^ Strachan, Tom; Read, Andrew P. (1999). "Glossary". Human Molecular Genetics (2nd ed.). New York: Wiley–Liss. ISBN 1-85996-202-5. PMID 21089233. http://www.ncbi.nlm.nih.gov/books/NBK7584/. [page needed]
- ^ Marchi, M. De; Carbonara, A. O.; Carozzi, F.; Massara, F.; Belforte, L.; Moldsatti, G. M.; Bisbocci, D.; Passarino, M. P. et al. (2008). "True hermaphroditism with XX/XY sex chromosome mosaicism: Report of a case". Clinical Genetics 10 (5): 265–72. doi:10.1111/j.1399-0004.1976.tb00047.x. PMID 991437.
- ^ Fitzgerald, PH; Donald, RA; Kirk, RL (1979). "A true hermaphrodite dispermic chimera with 46,XX and 46,XY karyotypes". Clinical genetics 15 (1): 89–96. doi:10.1111/j.1399-0004.1979.tb02032.x. PMID 759058.
- ^ Strachan, Tom; Read, Andrew P. (1999). "Chromosome abnormalities". Human Molecular Genetics (2nd ed.). New York: Wiley–Liss. ISBN 1-85996-202-5. PMID 21089233. http://www.ncbi.nlm.nih.gov/books/NBK7572/#A196. [page needed]
- ^ King R.C; Stansfield W.D. and Mulligan P.K. 2006. A Dictionary of Genetics. 7th ed, Oxford University Press. p282
- ^ https://health.google.com/health/ref/Mosaicism
- ^ Schwab, Angela L.; Tuohy, Thérèse M. F.; Condie, Michelle; Neklason, Deborah W.; Burt, Randall W. (2007). "Gonadal mosaicism and familial adenomatous polyposis". Familial Cancer 7 (2): 173–7. doi:10.1007/s10689-007-9169-1. PMID 18026870.
- ^ Lee, Tzumin; Luo, Liqun (1999). "Mosaic Analysis with a Repressible Cell Marker for Studies of Gene Function in Neuronal Morphogenesis". Neuron 22 (3): 451–61. doi:10.1016/S0896-6273(00)80701-1. PMID 10197526.
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Look at other dictionaries:
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mosaic evolution — mosaic evolution. См. мозаичная эволюция. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
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mosaic — /moʊˈzeɪɪk / (say moh zayik), /məˈzeɪɪk / (say muh zayik) noun 1. a picture or decoration made of small pieces of stone, glass, etc., of different colours, inlaid to form a design. 2. the process of producing it. 3. something resembling a mosaic… … Australian English dictionary
Mosaic — An individual or tissue containing two or more types of genetically different cells. All females are mosaics because of X chromosome inactivation (lyonization). Mosaic patterns can affect how genetic disorders are expressed. For example, about 5… … Medical dictionary
cauliflower mosaic virus — cauliflower mosaic virus. См. вирус мозаики цветной капусты. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
chromosome mosaic — chromosome mosaic. См. хромосомная химера. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
sex mosaic — sex mosaic. См. гинандроморф. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
hybrid tobacco mosaic virus — hybrid tobacco mosaic virus. См. гибридный вирус табачной мозаики. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.