Protein electrophoresis

Protein electrophoresis

In chemistry and medicine, protein electrophoresis (a.k.a. Immunoelectrophoresis) is a method of analysing a mixture of proteins by means of gel electrophoresis, mainly in blood serum (blood plasma is not suitable). Before the widespread use of electrophoresis gels, protein electrophoresis was performed on paper (sometimes in combination with paper chromotography).

Interpretation

There are two classes of blood proteins: serum albumin and globulin. They are generally equal in proportion, but albumin is much smaller and lightly negatively charged, leading to an accumulation of albumin on the electrophoretic gel. A small band before albumin represents transthyretin (also named prealbumin). Some forms of medication or body chemicals can cause their own band, usually small. Abnormal bands (spikes) are seen in monoclonal gammopathy of undetermined significance and multiple myeloma, and are useful in the diagnosis of these conditions.

The globulins are classified by their banding pattern (with their main representatives):

* The "alpha" (α) band consists of two parts, 1 and 2:
** α1 - α1-antitrypsin, α1-acid glycoprotein.
** α2 - haptoglobin, α2-macroglobulin, α2-antiplasmin, ceruloplasmin.
* The "beta" (β) band - transferrin, LDL, complement
* The "gamma" (γ) band - immunoglobulin (IgA, IgD, IgE, IgG and IgM). Paraproteins (in multiple myeloma) appear in this band.

See also

* Gel electrophoresis
* Paraprotein
* Electrofocusing
* QPNC-PAGE
* Immunofixation

External links

* [http://www3.interscience.wiley.com/cgi-bin/abstract/113343976/ABSTRACT Discontinuous native protein gel electrophoresis]
* [http://wolfson.huji.ac.il/purification/ Protein purification facility]


Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать курсовую

Look at other dictionaries:

  • protein electrophoresis — see serum protein e. and urine protein e …   Medical dictionary

  • Serum protein electrophoresis — (SPEP) is a laboratory test that examines specific proteins in the blood called globulins. Blood must first be collected, usually into an airtight vial or syringe. Electrophoresis is a laboratory technique where the blood serum (the fluid portion …   Wikipedia

  • Protein (disambiguation) — Protein is a class of biomolecules composed of amino acid chains.Protein may also refer to:Biochemistry* Antifreeze protein, class of polypeptides produced by certain fish, vertebrates, plants, fungi and bacteria * Conjugated protein, protein… …   Wikipedia

  • Electrophoresis (disambiguation) — Electrophoresis is both a phenomenon and a technique used in many scientific disciplines. Interface and Colloid ScienceElectrophoresis is the most known effect from electrokinetic phenomena. It is a motion of dispersed particles relative to a… …   Wikipedia

  • Electrophoresis — is the most well known electrokinetic phenomenon. It was discovered by Reuss in 1809. [Reuss, F.F. Mem.Soc.Imperiale Naturalistes de Moscow , 2, 327 1809] He observed that clay particles dispersed in water migrate under influence of an applied… …   Wikipedia

  • Protein fingerprinting — can refer to any of the several methods for identifying or differentiating proteins: *Peptide mass fingerprinting, a method developed in 1993 that uses protein mass spectrometry following SDS PAGE *Older techniques using two dimensional… …   Wikipedia

  • protein — proteinaceous /proh tee nay sheuhs, tee i nay /, proteinic, proteinous, adj. /proh teen, tee in/, n. 1. Biochem. any of numerous, highly varied organic molecules constituting a large portion of the mass of every life form and necessary in the… …   Universalium

  • Protein purification — is a series of processes intended to isolate a single type of protein from a complex mixture. Protein purification is vital for the characterisation of the function, structure and interactions of the protein of interest. The starting material is… …   Wikipedia

  • Protein methods — are the techniques used to study proteins.List of Protein MethodsThere are genetic methods for studying proteins, methods for detecting proteins, methods for isolating and purifying proteins and other methods for characterizing the structure and… …   Wikipedia

  • electrophoresis — n. 1. (Chem.) the motion of charged molecules or particles in a liquid medium under the influence of an electric field; particles with a positive charge move toward the cathode and negative to the anode. [WordNet sense 1] Syn: cataphoresis.… …   The Collaborative International Dictionary of English

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”