- Expression vector
An expression vector, otherwise known as an expression construct, is generally a
plasmidthat is used to introduce and express a specific geneinto a target cell. Expression vector allows production of large amounts of stable mRNA. Once the expression vector is inside the cell, the proteinthat is encoded by the gene is produced by the cellular transcription and translation machinery. The plasmid is engineered such that it contains a highly active promoterwhich causes the production of large amounts of mRNA.
After expression of the gene product, the purification of the protein is required; but since the vector is introduced to a host cell, the protein of interest should be purified from the proteins of the host cell. Therefore, to make purification process easy, the cloned gene should have a tag. This tag could be histidine (His) tag or any other marker peptide.
Expression vectors are used for
molecular biologytechniques such as site-directed mutagenesis. In general, DNA vectors that are used in many molecular biology gene cloningexperiments need not result in the expression of a protein. Expression vectors are often specifically designed to contain regulatory sequences that act as enhancerand promoterregions, and lead to efficient transcription of the gene that is carried on the expression vector. Expression vectors are basic tools for biotechnologyand the production of proteins such as insulinthat are important for medical treatments of specific diseases like diabetes.
*Alberts B, Johnson A, Lewis J, Raff M, Roberts K, Walter P (2002) "Molecular Biology of the Cell", 4th Edition. Garland Science ISBN 0-8153-4072-9.
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expression vector — noun In genetic manipulation, a vector that is specially constructed so that a large amount of the protein product, coded by an inserted sequence, can be made • • • Main Entry: ↑express … Useful english dictionary
expression vector — expression vector. См. экспрессионный вектор. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
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