COPI


COPI
COPI C-terminal domain
Identifiers
Symbol COPI_C
Pfam PF06957
InterPro IPR010714

COPI is a protein complex[1] that coats vesicles transporting proteins from the cis end of the Golgi complex back to the rough endoplasmic reticulum (ER), where they were originally synthesized and between golgi compartments. This type of transport is termed as retrograde transport. The name "COPI" refers to the specific coat protein complex that initiates the budding process on the cis-Golgi membrane. The coat consists of large protein subcomplexes that are made of seven different protein subunits.

Contents

Coat proteins

Coat protein, or COPI, is an ADP ribosylation factor (ARF)-dependent adaptor protein involved in membrane traffic.[2] COPI was first identified in retrograde traffic from the cis-Golgi to the rough endoplasmc reticulum (ER)[3][4] and is the most extensively studied of ARF-dependent adaptors. COPI consists of seven subunits which compose the heptameric protein complex.

The primary function of adaptors is the selection of cargo proteins for their incorporation into nascent carriers. In the case of the adaptor COPI, cargo containing the sorting motif KKXX interact with COPI to form carriers which are transported from the cis-Golgi to the rough ER.[5][6][7][8] Current views suggest that ARFs are also involved in the selection of cargo for incorporation into carriers.

Budding process

ADP ribosylation factor (ARF) is a GTPase involved in membrane traffic. There are 30 mammalian ARFs, and 29 in humans due to the loss of ARF2. ARF is post-translationally modified at the N-terminus by the addition of the fatty acid myristate.

ARF cycles between GTP and GDP-bound conformations. In the GTP-bound form, ARF conformation changes such that the myristate and hydrophobic N-terminal become more exposed and associate with the membrane. The interconversion between GTP and GDP bound states is mediated by ARF guanine nucleotide exchange factors (GEFs) and ARF GTPase activating proteins (GAPs). At the membrane, ARF-GTP is hydrolyzed to ARF-GDP by ARF GAPs. Once in the GDP-bound conformation, ARF converts to a less hydrophobic conformation and dissociates from the membrane. Soluble ARF-GDP is converted back to ARF-GTP by GEFs.

1. Luminal proteins: Proteins found in the lumen of the Golgi complex that need to be transported to the lumen of the ER contain the signal peptide KDEL. This sequence is recognized by a membrane-bound KDEL receptor. In yeast, this is Erd2p and in mammals it is KDELR. This receptor then binds to an ARF-GEF, a class of guanine nucleotide exchange factors. This protein in turn binds to the ARF. This interaction causes ARF to exchange its bound GDP for GTP. Once this exchange is made ARF binds to the cytosolic side of the cis-Golgi membrane.
2. Membrane proteins: Transmembrane proteins which reside in the ER contain sorting signals in their cytosolic tails which direct the protein to exit the Golgi and return to the ER. These sorting signals, or motifs, typically contain the amino acid sequence KKXX, which interact with COPI. The order in which adaptor proteins associate with cargo, or adaptor proteins associate with ARFs is unclear, however, in order to form a mature transport carrier coat protein, adaptor, cargo, and ARF must all associate.

Membrane deformation and carrier budding occurs following the collection of interactions described above. The carrier then buds off of the donor membrane, in the case of COPI this membrane is the cis-Golgi, and the carrier moves to the ER where it fuses with the acceptor membrane and its content is expelled.

References

  1. ^ MeSH Coat+Protein+Complex+I
  2. ^ Serafini T, Orci L, Amherdt M, Brunner M, Kahn RA, Rothman JE (1991). "ADP-ribosylation factor is a subunit of the coat of Golgi-derived COP-coated vesicles: a novel role for a GTP-binding protein.". Cell 67 (2): 239–53. doi:10.1016/0092-8674(91)90176-Y. PMID 1680566. 
  3. ^ Schekman R., Orci L. (1996). "Coat proteins and vesicle budding". Science 271 (5255): 1526–1533. doi:10.1126/science.271.5255.1526. PMID 8599108. 
  4. ^ Cosson P, Letourneur F (1997). "Coatomer (COPI)-coated vesicles: role in intracellular transport and protein sorting.". Curr Opin Cell Biol 9 (4): 484–7. doi:10.1016/S0955-0674(97)80023-3. PMID 9261053. 
  5. ^ Letourneur F, Gaynor EC, Hennecke S, Démollière C, Duden R, Emr SD et al. (1994). "Coatomer is essential for retrieval of dilysine-tagged proteins to the endoplasmic reticulum.". Cell 79 (7): 1199–207. doi:10.1016/0092-8674(94)90011-6. PMID 8001155. 
  6. ^ Sohn K, Orci L, Ravazzola M, Amherdt M, Bremser M, Lottspeich F et al. (1996). "A major transmembrane protein of Golgi-derived COPI-coated vesicles involved in coatomer binding.". J Cell Biol 135 (5): 1239–48. doi:10.1083/jcb.135.5.1239. PMC 2121093. PMID 8947548. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2121093. 
  7. ^ Sönnichsen B, Watson R, Clausen H, Misteli T, Warren G (1996). "Sorting by COP I-coated vesicles under interphase and mitotic conditions.". J Cell Biol 134 (6): 1411–25. doi:10.1083/jcb.134.6.1411. PMC 2120996. PMID 8830771. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2120996. 
  8. ^ Orci L, Stamnes M, Ravazzola M, Amherdt M, Perrelet A, Söllner TH et al. (1997). "Bidirectional transport by distinct populations of COPI-coated vesicles.". Cell 90 (2): 335–49. doi:10.1016/S0092-8674(00)80341-4. PMID 9244307. 

See also


Wikimedia Foundation. 2010.

Look at other dictionaries:

  • Copi — Nom de naissance Raul Damonte Botana Activités Écrivain (roman, théâtre) Dessinateur Naissance 20 novembre 1939 Buenos Aires (Argentine) Décès 14 décembre 1987 Paris ( …   Wikipédia en Français

  • Copi — Saltar a navegación, búsqueda Copi Nombre Raúl Damonte Botana Nacimiento 20 de noviembre de 1939 Buenos Aires …   Wikipedia Español

  • COPi — Связать? COPi (от англ. Cell Of Personal Information ячейка персональной информации) сетевой проект, сервис, позволяющий пользователям предоставлять свои персональные данные (имя, адреса электронной почты, веб сайтов, информацию об интересах,… …   Википедия

  • Copi — ist der Künstlername von Raúl Damonte Botana (* 20. November 1939 in Buenos Aires; † 14. Dezember 1987 in Paris), einem aus Argentinien stammenden Comiczeichner, Autor und Schauspieler. Schon als Kind kam Copi mit seinen Eltern, die nach Pérons… …   Deutsch Wikipedia

  • Copi — (ital.), d. i. Becher, eine der 4 Farben in der italienischen Karte, s. u. Spielkarten …   Pierer's Universal-Lexikon

  • Copi — This article is about the Argentine writer. For the protein, see COPI. Raúl Damonte Botana (November 20, 1939, Buenos Aires – December 14, 1987, Paris), better known by the nom de plume Copi (for copito de nieve , Spanish for little… …   Wikipedia

  • COPI — …   Википедия

  • COPI.ru — …   Википедия

  • COPi.ru — …   Википедия

  • Copi.ru — …   Википедия


Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”

We are using cookies for the best presentation of our site. Continuing to use this site, you agree with this.