- Intermediate filament
Intermediate filaments (IFs) are cytoskeletal structures formed by members of a family of related proteins called
keratin. Intermediate filaments have a diameter of 12 nanometers, which is between that of actin( microfilaments) and microtubules. Most types of intermediate filaments are located in the cytosolbetween the nuclear envelopeand the cell surface membrane. Nuclear lamins are localized to the cell nucleus.
The domain structure of IF molecules is conserved. Each protein has a non-alpha-helical (globular) domain at the N and C-termini which surrounds the
alpha-helicalrod domain. The basic building block for IFs is a parallel and in register dimer. The dimer is formed through the interaction of the rod domain to form a coiled coil. Cytoplasmic IF assemble into non-polar unit-length filaments which then assemble into longer structures.
The anti-parallel orientation of tetramers means that, unlike microtubules and microfilaments which have a plus end and a minus end, IFs lack polarity.
Cytoplasmic IF do not undergo
treadmillinglike microtubules and actin fibers, but they are dynamic. For a review see: [http://jcs.biologists.org/cgi/content/full/117/2/133] .
There are about 70 different genes coding for various intermediate filament proteins. However, different kinds of IFs share basic characteristics: they are all polymers that generally measure between 9-11 nm in diameter when fully assembled.
IF are subcategorized into six types based on similarities in amino acid sequence and
Types I and II - Acidic and Basic Keratins
These proteins are the most diverse among IFs and constitute type I (acidic) and type II (basic) IF proteins. The many
isoforms are divided in two groups:
* epithelial keratins (about 20) in
epithelialcells (image to right)
* trichocytic keratins (about 13) (
hair keratins) which make up hair, nails, horns and reptilian scales.
Regardless of the group, keratins are either acidic or basic. Acidic and basic keratins bind each other to form acidic-basic heterodimers and these heterodimers then associate to make a keratin filament.
There are four proteins classed as type III IF proteins which may form homo- or
DesminIFs are structural components of the sarcomeres in muscle cells.
*GFAP (glial fibrillary acidic protein) is found in
astrocytes and other glia.
Peripherinfound in peripheral neurons.
Vimentin, the most widely distributed of all IF proteins, can be found in fibroblasts, leukocytes, and blood vessel endothelial cells. They support the cellular membranes and keep some organelles in a fixed place within the cytoplasm.
Neurofilaments - the type IV family of intermediate filaments that is found in high concentrations along the axons of vertebrate neurons.
Type V - Nuclear Lamins
LaminsLamins are fibrous proteins having structural function in the cell nucleus.
In metazoan cells there are A and B type lamins which differ in their length and pI. Human cells have three differentially regulated genes.B-type lamins are present in every cell. B type lamins, B1 and B2, are expressed from the LMNB1 and LMNB2 genes on 5q23 and 19q13, respectively.A-type lamins are only expressed following
gastrulation. Lamin A and C are the most common A-type lamins and are splice variants of the LMNA gene found at 1q21.
These proteins localize to two regions of the nuclear compartment, the nuclear lamina -- a proteinaceous structure layer subjacent to the inner surface of the nuclear envelope and throughout the nucleoplasm in the nucleoplasmic "veil".
Comparison of the lamins to vertebrate cytoskeletal IFs shows that lamins have an extra 42 residues (six heptads) within coil 1b. The c-terminal tail domain contains a nuclear localization signal (NLS), an Ig-fold like domain, and in most cases a carboxy-terminal CaaX box that is isoprenylated and carboxymethylated (lamin C does not have a CAAX box). Lamin A is further processed to remove the last 15 amino acids and its farnesylated cysteine.
During mitosis, lamins are phosphorylated by MPF which drives the disassembly of the lamina and the nuclear envleope.
plasma membrane, some keratins interact with desmosomes (cell-cell adhesion) and hemidesmosomes (cell-matrix adhesion) via adapter proteins.
Filaggrinbinds to keratin fibers in epidermal cells. Plectinlinks vimentin to other vimentin fibers, as well as to microfilaments, microtubules, and myosinII.
Keratin filaments in epithelial cells link to
desmosomes through plakoglobin, desmoplakin, desmogleins and desmocollins; desminfilaments are connected in a similar way in heart muscle cells.
Diseases arising from mutations in IF genes
Epidermolysis bullosa simplex; K5 or K14 mutation
Laminopathiesare a family of diseases caused by mutations in nuclear lamins and include Hutchinson Gilford Progeria Syndromeand various lipodystrophies and cardiomyopathies among others.
* [http://www.interfil.org Human Intermediate Filament Database(HIFD)] , a comprehensive database of human intermediate filament proteins, their associated variations and diseases.
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