- Fertility factor
The Fertility factor (also known as F factor or sex factor) is a
bacterial DNAsequence that allows a bacterium to produce a sex pilus necessary for conjugation. It contains 20 "tra" (for "transfer") genes and a number of other genetic sequences responsible for incompatibility, replication, and other functions. The F factor is an episomeand can exist as an independent plasmidor integrate into the bacterial cell's genome. There are several names for the possible states:
*Hfr bacteria possess F factor integrated into the bacterial
*F+ bacteria possess F factor as a plasmid independent of the bacterial genome. The F plasmid contains only F factor DNA and no DNA from the bacterial genome.
* F' (F-prime) bacteria possess an F plasmid that also includes some DNA taken from the bacterial genome.
*F- bacteria do not contain F factor.
When an F+ cell conjugates with an F− cell, the result is two F+ cells, both capable of transmitting the plasmid further by conjugation. Unlike other plasmids, F factor is constitutive for transfer proteins due to the gene Tra J. This means that an F+ bacteria can always act as a donor cell. In the case of Hfr, the result are two Hfr cells. When F-prime plasmids are transferred to a recipient bacterial cell, they carry pieces of the donor's DNA that can become important in
recombination. Bioengineers have created F plasmids that can contain inserted foreign DNA; this is called a fosmid.
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