- Cysteine lactose electrolyte deficient agar
CLED agar (cystine lactose electrolyte deficient medium) is a valuable non-inhibitory growth medium used in the isolation and differentiation of urinary organisms. Being electrolyte deficient, it prevents the swarming of Proteus species. Cystine promotes the formation of cystine-dependent dwarf colonies. Lactose fermenters produce yellow colonies on CLED agar; non-lactose fermenters appear blue. It has a pH of approximately 7.3. CLED agar contains:
Peptone 4g/l 'Lab Lemco' powder 3g/l Tryptone 4g/l Lactose 10g/l L-Cystine 128 mg/l Bromothymol blue 20 mg/l Agar No. 1 15g/l Growth media / agar plates Selective mediaAlphaproteobacteriaBrucella abortus (Brucella agar)Betaproteobacteria Differential media Fungal media Nonselective media Other/ungrouped media
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