- Gene library
A gene library is a
populationof organisms, each of which carries a DNAmolecule that was inserted into a cloning vector. Ideally, all of the cloned DNA moleculesrepresent the entire genomeof the organism. A genelibrary is also called " gene bank". This term also represents the collection of all of the vectormolecules, each carrying piece of the chromosomalDNA of the organism, prior to the insertion of these molecules into the population of the host cell.
Types of Gene Libraries
# Plasmid libraries: Have a capacity of 10 kb.
# Phage libraries: Have a capacity of 23 kb.
# Cosmid libraries: Have a capacity of 45 kb.
# Yeast artificial chromosome (YAC) libraries: Have a capacity of 1000 kb.
Creating a library
DNA moleculesof an organismin interest are isolated. The DNA moleculesare then partially digested by an endonucleaserestriction enzyme. Sometimes, the DNA moleculesare digested to different lengths of time in order to ensure that all the geneshave been digested to manageable sizes. The digested DNA molecules are run on agarose electrophoresisfor which a suitable range of lengths of DNApieces are isolated and ligated to vector plasmids. Statistically, 99% of the geneswill be incoporated into the plasmids. The plasmidsthen can be taken up by suitable hosts.The hosts are kept in liquid media and can be frozen at -80°C for a long period of time. Usually the hosts are bacteriathat do not contain any plasmidsso as to be sensitive to antibiotics.
The process of subdividing
genomic DNAinto clonable element and inserting them into host is called creating a library, a clone bank or a gene bank. A complete library of host cell will contain all of the genomic DNA of the source organism.
* [http://www.biotecnika.googlepages.com/gene_library.html Gene library]
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