Tricine


Tricine
Tricine
Identifiers
CAS number 5704-04-1
PubChem 79784
ChemSpider 72078 YesY
ChEBI CHEBI:46759 YesY
Jmol-3D images Image 1
Properties
Molecular formula C6H13NO5
Molar mass 179.17 g/mol
Exact mass 179.079373
Melting point

182-184 °C (dec.)

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Except where noted otherwise, data are given for materials in their standard state (at 25 °C, 100 kPa)
Infobox references

Tricine is an organic compound that is used in buffer solutions. The name tricine comes from tris and glycine from which it was derived.[1] It is a zwitterionic amino acid with a useful buffering range of pH 7.4-8.8. It is a white crystalline powder that is moderately soluble in water. It has a pH of 4.4-5.2; its pKa1 at 25 °C is 2.3, while its pKa2 at 20 °C is 8.15.[1] Along with bicine, it is one of Good's buffering agents. Good first prepared tricine to buffer chloroplast reactions.

Applications

Tricine is a commonly used electrophoresis buffer and is also used in resuspension of cell pellets. It has a lower negative charge than glycine allowing it to migrate faster. In addition its high ionic strength causes more ion movement and less protein movement. This allows for low molecular weight proteins to be separated in lower percent acrylamide gels. Tricine has been documented in the separation of proteins in the range of 1 to 100 kDa by electrophoresis.[2] The tricine buffer at 25 mmol/L was found to be the most effective buffer among the ten tested for ATP assays using firefly luciferase.[3] Tricine has also been found to be an effective scavenger of hydroxyl radicals in a study of radiation-induced membrane damage.[4]

See also

References

  1. ^ a b Good, N.E., et al., Biochemistry, v. 5, 467 (1966).
  2. ^ Schaegger, H., and von Jagow, G., "Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa." "Anal. Biochem." 166(2), 368-379.
  3. ^ Webster, J. J., and Leach, F. R., "Optimization of the firefly luciferase assay for ATP." "J. Appl. Biochem.", 2:469-479.
  4. ^ Hicks, M., and Gebicki, J. M., "Rate constants for reaction of hydroxyl radicals with Tris, Tricine, and Hepes buffers." "FEBS Lett.", 199(1):92-94.

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