tRNA(also known as "transfer ribonucleic acid") to which its cognated amino acid is adhered. Their role is to deliver the amino acidto the ribosomewhere it will be incorporated into the polypeptidechain that is being produced.cite book | author = Berg J, Tymoczko JL, Stryer L | title = Biochemistry | publisher = W. H. Freeman | edition = 6th ed. | location = San Francisco | year = 2006 | isbn = 0716787245 ] A specific amino acid is added to each tRNA, which is crucial since it means that only that particular amino acid will be incorporated when the anticodon of that tRNA fits (can form a transient base pairwith) the next codon of the mRNAthat is being translated into protein.
The specific linkage of the correct amino acid to each tRNA is accomplished by
aminoacyl-tRNA synthetases. Due to the degeneracy of the genetic code, some of the different tRNAs have the same amino acid attached to them.
Aminoacyl-tRNA (also known as charged tRNA) is produced in two steps; amino acid activation and transfer. The first step is the adenylation of the amino acid, which forms aminoacyl-AMP:
Amino acid + ATP ↔ Aminoacyl-AMP + PPi
Then, the amino acid residue is transferred to the tRNA:
Aminoacyl-AMP + tRNA ↔ Aminoacyl-tRNA + AMP
The net reaction is
Amino acid + ATP + tRNA ↔ Aminoacyl-tRNA + AMP + PPi
The net reaction is only energetically favourable because the pyrophosphate is hydrolysed; that reaction is highly energetically favourable and drives the other reactions. All of these reactions take place inside the aminoacyl-tRNA synthetase specific for that tRNA.
Drugs that target aminoacyl-tRNA binding to ribosomal subunit
Certain drugs like
Erythromycinprevent the aminoacyl-tRNA from binding to the ribosomal subunitin prokaryotes.
Aminoacyl tRNA synthetase
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