Attenuator (genetics)

Attenuator (genetics)

The attenuator plays an important regulatory role in prokaryotic cells because of the absence of the nucleus in prokaryotic organisms. The attenuator refers to a specific regulatory sequence that, when transcribed into RNA, forms hairpin structures to stop translation when certain conditions are not met.

An example is the "trp" gene in bacteria. When there is a high level of tryptophan in the region, the bacterium may not want to synthesize more because it wants to save energy. When the RNA polymerase binds and transcribes the "trp" gene, the ribosome will start translating. (This differs from eukaryotic cells, where RNA must exit the nucleus before translation starts.) The attenuator sequence, which is located between the mRNA leader sequence (5' UTR) and trp operon gene sequence, contains four domains, where domain 3 can pair with domain 2 or domain 4.

The attenuator sequence at domain 1 contains instruction for peptide synthesis that requires tryptophans. A high level of tryptophan will permit ribosomes to translate the attenuator sequence domains 1 and 2, allowing domains 3 and 4 to form a hairpin structure, which results in termination of transcription of the trp operon. Since the protein coding genes are not transcribed due to rho independent termination, no tryptophan is synthesised.

In contrast, a low level of tryptophan means that the ribosome will stall at domain 1, causing the domains 2 and 3 to form a different hairpin structure that does not signal termination of transcription. Therefore the rest of the operon will be transcribed and translated, so that tryptophan can be produced. Thus, domain 4 is an attenuator. Without domain 4, translation can continue regardless of the level of tryptophan. [http://www.ndsu.nodak.edu/instruct/mcclean/plsc431/prokaryo/prokaryo3.htm] The attenuator sequence has its codons translated into a leader peptide, but is not part of the trp operon gene sequence. The attenuator allows more time for the attenuator sequence domains to form loop structures, but does not produce a protein that is used in later tryptophan synthesis.

Attenuation is a second mechanism of negative feedback in the trp operon. While the TrpR repressor decreases transcription by a factor of 70, attenuation can further decrease it by a factor of 10, thus allowing accumulated repression of about 700-fold. Attenuation is made possible by the fact that in prokaryotes (which have no nucleus), the ribosomes begin translating the mRNA while RNA polymerase is still transcribing the DNA sequence. This allows the process of translation to directly affect transcription of the operon.

At the beginning of the transcribed genes of the trp operon is a sequence of 140 nucleotides termed the leader transcript (trpL). This transcript includes four short sequences designated 1-4. Sequence 1 is partially complementary to sequence 2, which is partially complementary to sequence 3, which is partially complementary to sequence 4. Thus, three distinct secondary structures (hairpins) can form: 1-2, 2-3 or 3-4. The hybridization of strands 1 and 2 to form the 1-2 structure prevents the formation of the 2-3 structure, while the formation of 2-3 prevents the formation of 3-4. The 3-4 structure is a transcription termination sequence, once it forms RNA polymerase will disassociate from the DNA and transcription of the structural genes of the operon will not occur.

Part of the leader transcript codes for a short polypeptide of 14 amino acids, termed the leader peptide. This peptide contains two adjacent tryptophan residues, which is unusual, since tryptophan is a fairly uncommon amino acid (about one in a hundred residues in a typical E. coli protein is tryptophan). If the ribosome attempts to translate this peptide while tryptophan levels in the cell are low, it will stall at either of the two trp codons. While it is stalled, the ribosome physically shields sequence 1 of the transcript, thus preventing it from forming the 1-2 secondary structure. Sequence 2 is then free to hybridize with sequence 3 to form the 2-3 structure, which then prevents the formation of the 3-4 termination hairpin. RNA polymerase is free to continue transcribing the entire operon. If tryptophan levels in the cell are high, the ribosome will translate the entire leader peptide without interruption and will only stall during translation termination at the stop codon. At this point the ribosome physically shields both sequences 1 and 2. Sequences 3 and 4 are thus free to form the 3-4 structure which terminates transcription. The end result is that the operon will be transcribed only when tryptophan is unavailable for the ribosome, while the trpL transcript is constitutively expressed.

To ensure that the ribosome binds and begins translation of the leader transcript immediately following its synthesis, a pause site exists in the trpL sequence. Upon reaching this site, RNA polymerase pauses transcription and apparently waits for translation to begin. This mechanism allows for synchronization of transcription and translation, a key element in attenuation.

A similar attenuation mechanism regulates the synthesis of histidine, phenylalanine and threonine.

Attenuation as a control in gene expression

Attenuation was first observed by Charles Yanofsky in the trp operon of "E. coli". The first observation was linked to two separate scientific facts. Mutations which knocked out the trp R (repressor) gene still showed some regulation of the trp operon (these mutants were not fully induced/repressed by tryptophan). The total range of trp operon regulation is about 700 X (on/off). When the trp repressor was knocked out, one still got about 10 X regulation by the absence or presence of trp. When the sequence of the beginning of the trp operon was determined an unusual open reading frame (ORF) was seen immediately preceding the ORFs for the known structural genes for the tryptophan biosynthetic enzymes.The general structural information shown below was observed from the sequence of the trp operon.

First, Yanofsky observed that the ORF contained two tandem Trp codons and the protein had a Trp percent composition which was about 10X normal. Second, the mRNA in this region contained regions of dyad symmetry which would allow it to form two mutually exclusive secondary structures. One of the structures looked exactly like a rho-independent transcription termination signal. The other secondary structure, if formed, would prevent the formation of this secondary structure and thus the terminator. This other structure is called the "preemptor".

Mechanism of attenuation in the trp operon.

The proposed mechanism of how this mRNA secondary structure and the trp leader peptide could regulated transcription of the trp biosynthetic enzymes includes the following.

* RNAP initiates transcription of the trp promoter. * RNAP pauses at about nucleotide 90 at a secondary structure (?the first one shown above?). * Ribosomes engage this nascent mRNA and initiate translation of the leader peptide. o RNAP is then "released" from its pause and continues transcription. * When RNAP reaches the region of the potential terminator, whether it continues or not is dependent on the position of the ribosome "trailing behind". o If the ribosome, stalls at the tandem Trp codons, waiting for the appropriate tRNA, region 1 is sequestered within the ribosome and thus cannot base pair with region 2. This means that region 2 and 3 become based paired before region 4 can be transcribed. This forces region 4 when it is made to be single stranded, preventing the formation of the region 3/4 terminator structure. Transcription will then continue. o If the ribosome translates the leader peptide with no hesitation, it then covers a portion of region 2 preventing it from base pairing with region 3. Then when region 4 is transcribed, it forms a stem and loop with region 3 and transcription is terminated, generating a ca. 140 base transcript. * This mechanism of control measures the amount of available, charged Trp-tRNA.

The location of ribosomes determines which alternate secondary structures form.

Other operons controlled by attenuation.

The discovery of this type of mechanism to control the expression of genes in a biosynthetic operon lead to its rediscovery in a wide variety of such operons for which repressors had never been discovered. For example:Other Operons Controlled by Attenuation Operon Leader peptideHistidine MTRVQFKHHHHHHHPD stopThreonine MKRISTTITTTITITTGNGAG stopIlv (GEDA) MTALLRVISLVVISVVVIIIPPCGAALGRGKA stopLeu MSHIVRFTGLLLLNAFIVRGRPVGGIQH stopPhenylalanine MKHIPFFFAFFFTFP stopIlvB MTTSMLNAKLLPTAPSAAVVVVRVVVVVGNAP stop

Single-letter abbreviations for amino acids followed by their normal percentage composition in a"average" population of proteins. Ala = A (8.6) Arg = R (4.9)Gly = G (8.4) Ile = I (4.5)Leu = L (7.4) Asn = N (4.3)Ser = S (7.0) Gln = Q (3.9)Val = V (6.6) Phe = F (3.6)Lys = K (6.6) Tyr = Y (3.4)Thr = T (6.1) Cys = C (2.9)Glu = E (6.0) His = H (2.0)Asp = D (5.5) Met = M (1.7)Pro = P (5.2) Trp = W (1.3)

References Yanofsky, Nature 289:751 (1981) Read Genes VI pp374-380


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См. также в других словарях:

  • attenuator — attenuator. См. аттенюатор. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Attenuator — An attenuator could mean: * Attenuator (electronics), an electronic device that reduces the amplitude of a signal * Attenuator (genetics), a specific regulatory sequence transcribed into RNA * Impact attenuator, used on highways as a crumple zone …   Wikipedia

  • List of genetics-related topics — This is a list of terms related to genetics. NOTOC # * 3 end * 5 end A * Acentric chromosome * Achondroplasia * Active site * Adam s Curse * Adaptation * Adenine * Adenosine * Adenovirus * Adenosine diphosphate (ADP) * Ala * Alagille syndrome *… …   Wikipedia

  • аттенюатор — attenuator аттенюатор. Регулируемый терминатор транскрипции бактерий, например, между генами trpO и trpE триптофанового оперона E.coli содержится А., который в условиях избытка триптофана обеспечивает снижение уровня синтеза trp мРНК. (Источник:… …   Молекулярная биология и генетика. Толковый словарь.

  • аттенюация — attenuation аттенюация. Механизм регуляции транскрипции генов, известный у ряда бактерий; А. является следствием преждевременной терминации синтеза мРНК в определенном участке гена аттенюаторе <attenuator>. (Источник: «Англо русский… …   Молекулярная биология и генетика. Толковый словарь.

  • лидерная последовательность — leader sequence лидерная последовательность. Нетранслируемая последовательность мРНК, расположенная между ее 5’ концом и инициаторным кодоном АУГ; Л.п. может включать ряд регуляторных элементов например, аттенюатор <attenuator> (как в… …   Молекулярная биология и генетика. Толковый словарь.

  • Attenuation — This article is about attenuation in physics. For other uses, see Attenuation (disambiguation). In physics, attenuation (in some contexts also called extinction) is the gradual loss in intensity of any kind of flux through a medium. For instance …   Wikipedia

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  • List of genetic engineering topics — NOTOC # 3 end 5 end A Acentric chromosome Achondroplasia Active site Adam s Curse Adaptation Adenine Adenosine Adenosine diphosphate Adenovirus ADP Ala Alagille syndrome Albino Alcoholism Alkylating agent Allele Allele frequency Alleles… …   Wikipedia

  • operon — /op euh ron /, n. Genetics. a set of two or more adjacent cistrons whose transcription is under the coordinated control of a promoter, an operator, and a regulator gene. [1960 65; < F opéron, equiv. to opér(er) to work, OPERATE + on ON1] * * *… …   Universalium


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