Microfilament

Microfilament
Actin cytoskeleton of mouse embryo fibroblasts, stained with Fluorescein isothiocyanate-phalloidin

Microfilaments ( or actin filaments) are the thinnest filaments of the cytoskeleton, a structure found in the cytoplasm of all eukaryotic cells. These linear polymers of actin subunits are flexible and relatively strong, resisting buckling by multi-piconewton compressive forces and filament fracture by nanonewton tensile forces. Microfilaments are highly versatile, functioning in cell crawling, amoeboid movement, and changes in cell shape. In inducing this cell motility, one end of the actin filament elongates while the other end contracts, presumably by myosin II molecular motors.[1] Additionally, they function as part of actomyosin-driven contractile molecular motors, wherein the thin filaments serve as tensile platforms for myosin's ATP-dependent pulling action in muscle contraction and uropod advancement.

Contents

Organization

Actin filaments are assembled in two general types of structures: bundles and networks. Bundles can be composed of polar filament arrays, in which all barbed ends point to the same end of the bundle, or non-polar arrays, where the barbed ends point towards both ends. A class of actin-binding proteins, called cross-linking proteins, dictate the formation of these structures. Cross-linking proteins determine filament orientation and spacing in the bundles and networks. These structures are regulated by many other classes of actin-binding proteins, including motor proteins, branching proteins, severing proteins, polymerization promoters, and capping proteins.

In vitro self-assembly

Measuring approximately 6 nm in diameter,[2] microfilaments are the thinnest fibers of the cytoskeleton. They are polymers of actin subunits, which as part of the fiber are referred to as filamentous actin, or F-actin. Each microfilament is made up of two helix, interlaced strands of subunits. Much like microtubules, actin filaments are polarized. Electron micrographs have provided evidence of their fast-growing barbed-ends and their slow-growing pointed-end. This polarity has been determined by the pattern created by the binding of myosin S1 fragments: they themselves subunits of the larger myosin II protein complex. The pointed end is commonly referred to as the minus (-) end and the barbed end is referred to as the plus (+) end.

In vitro actin polymerization, or nucleation, starts with the self-association of three G-actin monomers to form a trimer. ATP-bound actin then itself binds the barbed end, and the ATP is subsequently hydrolyzed. ATP hydrolysis occurs with a half time of about 2 seconds,[3] while the half time for the dissociation of the inorganic phosphate is about 6 minutes,.[3] This autocatalyzed event reduces the binding strength between neighboring subunits, and thus generally destabilizes the filament. In vivo actin polymerization is catalyzed by a new class of filament end-tracking molecular motors known as actoclampins. Recent evidence suggests that the rate of ATP hydrolysis and the rate of monomer incorporation are strongly coupled.

Subsequently, ADP-actin dissociates slowly from the pointed end, a process significantly accelerated by the actin-binding protein, cofilin. ADP bound cofilin severs ADP-rich regions nearest the (–)-ends. Upon release, the free actin monomer slowly dissociates from ADP, which in turn rapidly binds to the free ATP diffusing in the cytosol, thereby forming the ATP-actin monomeric units needed for further barbed-end filament elongation. This rapid turnover is important for the cell's movement. End-capping proteins such as CapZ prevent the addition or loss of monomers at the filament end where actin turnover is unfavorable, such as in the muscle apparatus.

Mechanism of force generation

As a result of ATP hydrolysis, filaments elongate approximately 10 times faster at their barbed ends than their pointed ends. At steady-state, the polymerization rate at the barbed end matches the depolymerization rate at the pointed end, and microfilaments are said to be treadmilling. Treadmilling results in elongation in the barbed end and shortening in the pointed-end, so that the filament in total moves. Since both processes are energetically favorable, this means force is generated, the energy ultimately coming from the hydrolysis of this molecular unit of energy currrency.[1]

Actin in cells

Intracellular acting cytoskeletal assembly and disassembly are tightly regulated by cell signaling mechanisms. Many signal transduction systems use the actin cytoskeleton as a scaffold, holding them at or near the inner face of the peripheral membrane. This subcellular location allows immediate and exquisite responsiveness to transmembrane receptor action and the resulting cascade of signal-processing enzymes. Because actin monomers must be recycled to sustain high rates of actin-based motility during chemotaxis, cell signalling is believed to activate cofilin, the actin-filament depolymerizing protein which, as stated, binds to ADP-rich actin subunits nearest the filament's pointed-end and promotes filament fragmentation, with concomitant depolymerization in order to liberate actin monomers. The protein profilin enhances the ability of monomers to assemble by stimulating the exchange of actin-bound ADP for solution-phase ATP to yield Actin-ATP and ADP. In most animal cells, monomeric actin is bound to profilin and thymosin-beta4, both of which preferentially bind with one-to-one stoichiometry to ATP-containing monomers. Although thymosin-beta4 is strictly a monomer-sequestering protein, the behavior of profilin is far more complex. Profilin is transferred to the leading edge by virtue of its PIP2 binding site, and it employs its poly-L-proline binding site to dock onto end-tracking proteins. Once bound, Profilin-Actin-ATP is loaded into the monomer-insertion site of actoclampin motors. Another important component in filament formation is the Arp2/3 complex, which binds to the side of an already existing filament (or "mother filament"), where it nucleates the formation of a new daughter filament at a 70 degree angle relative to the mother filament, effecting a fan-like branched filament network.

Microfilament associated proteins

In non-muscle cells, actin filaments are formed proximal to membrane surfaces. Their formation and turnover are regulated by many proteins, including:

  • Filament end-tracking protein (e.g., formins, VASP, N-WASP)
  • Filament-nucleator known as the Actin-Related Protein-2/3 (or Arp2/3) complex
  • Filament cross-linkers (e.g., α-actinin and fascin)
  • Actin monomer-binding proteins profilin and thymosin-β4
  • Filament barbed-end cappers such as Capping Protein and CapG, etc.
  • Filament-severing proteins like gelsolin.
  • Actin depolymerizing proteins such as ADF/cofilin.

The actin filament network in non-muscle cells is highly dynamic. As first proposed by Dickinson & Purich (Biophysical Journal 92: 622–631), the actin filament network is arranged with the barbed-end of each filament attached to the cell's peripheral membrane by means of clamped-filament elongation motors, the above-mentioned "actoclampins", formed from a filament barbed-end and a clamping protein (formins, VASP, Mena, WASP, and N-WASP). The primary substrate for these elongation motors is Profilin-Actin-ATP complex which is directly transferred to elongating filament ends (Dickinson, Southwick & Purich, 2002). The pointed-end of each filament is oriented toward the cell's interior. In the case of lamellipodial growth, the Arp2/3 complex generates a branched network, and in filopodia a parallel array of filaments is formed.

Actin acts as a track for myosin motor motility

Myosin motors are intracellular ATP-dependent enzymes that bind to and move along actin filaments. Various classes of myosin motors have very different behaviors, including exerting tension in the cell and transporting cargo vesicles.

A proposed model – actoclampins track filament ends

One proposed model[4] suggests the existence of actin filament barbed-end-tracking molecular motors termed "actoclampin." The proposed actoclampins generate the propulsive forces needed for actin-based motility of lamellipodia, filopodia, invadipodia, dendritic spines, intracellular vesicles, and motile processes in endocytosis, exocytosis, podosome formation, and phagocytosis. Actoclampin motors also propel such intracellular pathogens as Listeria monocytogenes, Shigella flexneri, Vaccinia and Rickettsia. When assembled under suitable conditions, these end-tracking molecular motors can also propel biomimetic particles.

The term actoclampin is derived from acto- to indicate the involvement of an actin filament, as in actomyosin, and clamp to indicate a clasping device used for strengthening flexible/moving objects and for securely fastening two or more components, followed by the suffix -in to indicate its protein origin. An actin filament end-tracking protein may thus be termed a clampin.

Dickinson and Purich (2002) recognized that prompt ATP hydrolysis could explain the forces achieved during actin-based motility. They proposed a simple mechanoenzymatic sequence known as the Lock, Load & Fire Model, in which an end-tracking protein remains tightly bound ("locked" or clamped) onto the end of one sub-filament of the double-stranded actin filament. After binding to Glycyl-Prolyl-Prolyl-Prolyl-Prolyl-Prolyl-registers on tracker proteins, Profilin-ATP-actin is delivered ("loaded") to the unclamped end of the other sub-filament, whereupon ATP within the already clamped terminal subunit of the other subfragment is hydrolyzed ("fired"), providing the energy needed to release that arm of the end-tracker, which then can bind another Profilin-ATP-actin to begin a new monomer-addition round.

===Steps involved The following steps describe one force-generating cycle of an actoclampin molecular motor:

  1. The polymerization cofactor profilin and the ATP·actin combine to form a profilin-ATP-actin complex that then binds to the end-tracking unit
  2. The cofactor and monomer are transferred to the barbed-end of an actin already clamped filament
  3. The tracking unit and cofactor dissociate from the adjacent protofilament, in a step that can be facilitated by ATP hydrolysis energy to modulate the affinity of the cofactor and/or the tracking unit for the filament; and this mechanoenzymatic cycle is then repeated, starting this time on the other sub-filament growth site.

When operating with the benefit of ATP hydrolysis, AC motors generate per-filament forces of 8–9 pN, which is far greater than the per-filament limit of 1–2 pN for motors operating without ATP hydrolysis (Dickinson and Purich, 2002, 2006; Dickinson, Caro and Purich, 2004). The term actoclampin is generic and applies to all actin filament end-tracking molecular motors, irrespective of whether they are driven actively by an ATP-activated mechanism or passively.

Some actoclampins (e.g., those involving Ena/VASP proteins, WASP, and N-WASP) apparently require Arp2/3-mediated filament initiation to form the actin polymerization nucleus that is then "loaded" onto the end-tracker before processive motility can commence. To generate a new filament, Arp2/3 requires a "mother" filament, monomeric ATP-actin, and an activating domain from Listeria ActA or the VCA region of N-WASP. Ther Arp2/3 complex binds to the side of the mother filament, forming a Y-shaped branch having a 70 degree angle with respect to the longitudinal axis of the mother filament. Then upon activation by ActA or VCA, the Arp complex is believed to undergo a major conformational change, bringing its two actin-related protein subunits near enough to each other to generate a new filament gate. Whether ATP hydrolysis may be required for nucleation and/or Y-branch release is a matter under active investigation.

References

  1. ^ a b Keith Roberts, Martin Raff, Bruce Alberts, Peter Walter, Julian Lewis and Alexander Johnson, Molecular Biology of the Cell, 4th Edition, Routledge, March, 2002, hardcover, 1616 pages, 7.6 pounds, ISBN 0-8153-3218-1
  2. ^ Fuchs E, Cleveland DW (January 1998). "A structural scaffolding of intermediate filaments in health and disease". Science 279 (5350): 514–9. doi:10.1126/science.279.5350.514. PMID 9438837. http://www.sciencemag.org/cgi/pmidlookup?view=long&pmid=9438837. 
  3. ^ a b Pollard T. D., Earnshaw W. D. (2004). Cell Biology (First ed.). SAUNDERS. ISBN 1-4160-2388-7. 
  4. ^ Richard B. Dickinson, Luzelena Caro and Daniel L. Purich, Force Generation by Cytoskeletal Filament End-Tracking Proteins, Biophys J. 2004 October; 87(4): 2838–2854.

External links



Wikimedia Foundation. 2010.

Игры ⚽ Поможем сделать НИР

Look at other dictionaries:

  • Microfilament — Filament d actine Un filament d actine, ou microfilament, est un homopolymère d actine, protéine de 42 kDa (Unité de masse atomique). C est un constituant essentiel du cytosquelette des cellules eucaryotes, ainsi que des fibres musculaires. L… …   Wikipédia en Français

  • microfilament — noun Date: 1963 any of the minute actin containing protein filaments of eukaryotic cytoplasm that function in maintaining structure and in intracellular movement …   New Collegiate Dictionary

  • Microfilament — G Aktin (PDB code: 1j6z) mit ADP und Kation F Aktin; Darstellung von 13 Aktin Untereinheiten (basierend auf dem …   Deutsch Wikipedia

  • microfilament — Cytoplasmic filament, 5 7nm thick, of F actin that can be decorated with HMM; may be laterally associated with other proteins (tropomyosin, a actinin) in some cases, and may be anchored to the membrane. Microfilaments are conspicuous in adherens… …   Dictionary of molecular biology

  • microfilament — microfilamentous /muy kroh fil euh men teuhs/, adj. /muy kreuh fil euh meuhnt/, n. Cell Biol. a minute, narrow tubelike cell structure composed of a protein similar to actin, occurring singly and in bundles, involved in cytoplasmic movement and… …   Universalium

  • microfilament — noun A very fine (thin) filament …   Wiktionary

  • microfilament — The finest filamentous element of the cytoskeleton, having a diameter of about 5 nm and consisting primarily of actin. SEE ALSO: actin filament. * * * mi·cro·fil·a·ment .mī krō fil ə mənt n any of the minute actin containing protein filaments… …   Medical dictionary

  • microfilament — mi·cro·filament …   English syllables

  • microfilament — mi•cro•fil•a•ment [[t]ˌmaɪ krəˈfɪl ə mənt[/t]] n. cbl a tubelike protein structure that is involved in cell movement and changes in cell shape • Etymology: 1960–65 mi•cro•fil•a•men•tous ˌmaɪ kroʊˌfɪl əˈmɛn təs adj …   From formal English to slang

  • microfilament — ˌ noun Etymology: micr + filament : any of the minute actin containing protein filaments that are widely distributed in the cytoplasm of eukaryotic cells, help maintain their structural framework, and play a role in the movement of cell… …   Useful english dictionary

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”