PBluescript

PBluescript

In genetics, pBluescript (pBS) or pBluescript II is a commercially available phagemid containing several useful sequences for use in cloning with bacteriophage. The sequences include a polylinker sequence (MCS), antibiotic resistance sequence to ampicillin and an "E. coli" and f1 helper phage origin of replication. The polylinker sequence is located within a LacZ controlled gene designed to provide a blue coloration when expressed in bacteria. This is usually achieved via IPTG found in agarose growth media used to culture bacteria with pBS. If the gene is disrupted by successful insertion of a DNA sequence, the bacteria exhibit a white coloration in Blue white screening, distinguishing successful recombination from those phagemids which were not altered. These recombinant plasmids can then be used in a variety of molecular techniques. Gene expression, genomic library etc are some applications which can be used for the pBS recombinants.

The pBluescript cloning system is a product of [http://www.stratagene.com/homepage/ Stratagene] .


Wikimedia Foundation. 2010.

Игры ⚽ Нужно решить контрольную?

Look at other dictionaries:

  • pBluescript — In genetics, pBluescript (pBS) or pBluescript II is a commercially available phagemid containing several useful sequences for use in cloning with bacteriophage. The sequences include a polylinker sequence (MCS), antibiotic resistance sequence to… …   Wikipedia

  • Bluescript — (= pBluescript) Proprietary plasmid, sold by Stratagene. Very widely used …   Dictionary of molecular biology

  • PGreen — The pGreen plasmids were first described in 2000 [ [http://www.springerlink.com/content/p264110437m2k479 pGreen: a versatile and flexible binary Ti vector for Agrobacterium mediated plant transformation, ] Plant Molecular Biology 42: 819 832,… …   Wikipedia

  • PBS (disambiguation) — PBS may refer to: in broadcasting: Public Broadcasting Service, an American non profit television service 3PBS (106.7FM), an Australian community (Melbourne) radio station Pacific Broadcasting Systems, a Filipino radio network Palestine… …   Wikipedia

  • Blue white screen — The blue white screen is a molecular technique that allows for the detection of successful ligations in vector based gene cloning. DNA of interest is ligated into a vector. The vector is then transformed into competent cell (bacteria). The… …   Wikipedia

  • phagemid — Bacteriophage whose genome contains a plasmid that can be excised by co infection of the host with a Helper phage. Useful as vectors for library production, as the library can be amplified and screened as phage, but the inserts of selected… …   Dictionary of molecular biology

  • Plasmidkopienzahl — Die Plasmidkopienzahl beschreibt die Zahl an Plasmiden einer Art pro Zelle. Man unterscheidet zwischen low copy Plasmidkopienzahl zwischen 1–12 pro Zelle medium copy Plasmidkopienzahl zwischen 15–20 pro Zelle high copy Plasmidkopienzahl zwischen… …   Deutsch Wikipedia

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”