- 4Pi Microscope
A 4Pi microscope is a
confocal microscopewith two opposing lenses. It is used for high resolution imaging of fluorescence.
It can be operated in three different ways:In a 4Pi microscope of type A, the coherent superposition of excitation light is used to generate the increased resolution. The emission light is either detected from one side only or in an incoherent superposition from both sides.In a 4Pi microscope of Type B, only the emission light is interfering.When operated in the Type C mode, both excitation and emission light are allowed to interfere leading to the highest possible resolution increase (~7 fold along the optic axis as compared to wide field fluorescence microscopy). A typical axial resolution of a 4Pi microscope is about 100 nm.
Typically (but not inevitably)
two-photon excitation microscopyis used in a 4Pi microscope in combination with the emission pinhole to lower the side lobes of the point spread function.
The method was pioneered by [http://www.4Pi.de Stefan Hell and his co-workers] . It is related to the method of I5 microscopy pioneered by [http://bioeng.berkeley.edu/graduate/cvs/Gustafsson.html Mats Gustafsson] .
Wikimedia Foundation. 2010.
См. также в других словарях:
4Pi — The expression 4Pi may refer to: * System 4 Pi, an IBM family of avionics * 4Pi Microscope, a microscope that uses interference and fluorescence] computers * 4 times;π = 12.56637..., the solid angle of a complete sphere measured in steradians … Wikipedia
4Pi-Mikroskop — Schema eines 4Pi Mikroskops. Ein 4Pi Mikroskop ist eine Variante des Konfokalmikroskops, das eine höhere Auflösung besitzt als die bei normalen konfokalen Mikroskopen übliche Auflösung von etwa 200 nm in seitlicher und 500–700 nm in… … Deutsch Wikipedia
4Pi STED microscopy — The 4Pi STED microscope is the result of combining the two unrelated concepts of STED and 4Pi microscopy. Here, the fluorescent sample is placed in the common focus of two opposing lenses, but excitation and detection are performed through a… … Wikipedia
Fluorescence microscope — An upright fluorescence microscope (Olympus BX61) with the fluorescent filter cube turret above the objective lenses, coupled with a digital camera. A fluorescence microscope is an optical microscope used to study properties of organic or… … Wikipedia
Near-field scanning optical microscope — Near field scanning optical microscopy (NSOM/SNOM) is a microscopic technique for nanostructure investigation that breaks the far field resolution limit by exploiting the properties of evanescent waves. This is done by placing the detector very… … Wikipedia
Multiphoton fluorescence microscope — A multiphoton fluorescence microscope (MFM) is a specialized optical microscope. Contents 1 Description 2 See also 3 References 4 External links … Wikipedia
Christoph Cremer — (born in Freiburg im Breisgau, Germany) is a German physicist and professor at the Ruprecht Karls University Heidelberg, who has successfully overcome the conventional limit of resolution that applies to light based investigations (the Abbe… … Wikipedia
Microscopy — is the technical field of using microscopes to view samples and objects that cannot be seen with the unaided eye (objects that are not within the resolution range of the normal eye). There are three well known branches of microscopy, optical,… … Wikipedia
Dispersion staining — Contents 1 Dispersion Staining 1.1 Becke Line Dispersion Staining 1.2 Oblique Illumination Dispersion Staining 1.3 Darkfield Illumination Dispersion Staining … Wikipedia
Confocal microscopy — Diagnostics MeSH D018613 OPS 301 code 3 301 … Wikipedia