- Romanowsky stain
Romanowsky staining was a prototypical staining technique that was the forerunner of several distinct but similar methods, including Giemsa, Jenner, Wright, and Leishman stains, which are used to differentiate cells in pathologic specimens.
Ehrlich had used mixtures of acidic and basic dyes for this purpose in 1879: in 1891 Romanowsky and
Malakowskyindependently developed a technique using a mixture of EosinY and oxidated Methylene Bluethat was also useful for this purpose. Because the aqueous dye solutions were unstable, methanolwas introduced as a solvent, and Leishman (in 1901) and Wright (in 1902) advocated use of methanol as a fixative prior to staining. Giemsa in 1902 improved this technique by standardizing the dye solutions and adding glycerolto increase solubility and stability.
The oxidation of Methylene Blue in aqueous solution using heat and alkali produces a mixture of Azure A, Azure B, Methylene Violet and Methylene Blue. Eosin Y is then added to produce a "neutral" dye. The precipitate is then dissolved in a mixture of methanol and glycerol to form a stock solution: this is diluted with water or an aqueous buffer to form a working solution that is used in the preparation of pathology specimens.
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Romanowsky stain — Ro·ma·now·sky stain or Ro·ma·now·sky s stain also Ro·ma·nov·sky stain or Ro·ma·nov·sky s stain .rō mə nȯf skē(z) n a stain made from water soluble eosin, methylene blue, and absolute methanol and used in parasitology Romanowsky Dimitri… … Medical dictionary
romanowsky stain — |rōmə|nȯfskē noun Usage: usually capitalized R Etymology: after Dimitri L. Romanowsky died 1921 Russ. physician : a stain made from water soluble eosin, methylene blue, and absolute methanol and used in parasitology … Useful english dictionary
stain — 1. To discolor. 2. To color; to dye. 3. A discoloration. 4. A dye used in histologic and bacteriologic technique. 5. A procedure in which a dye or combination of dyes and reagents is used to color the constituents of cells and tissues. For… … Medical dictionary
Romanowsky (Romanovsky) stain (method) — Ro·ma·now·sky (Ro·ma·nov·sky) stain (method) (ro″mə nouґske) (ro″mə nofґske) [Dimitri Leonidovich Romanowsky (or Romanovsky), Russian physician, 1861â€“1921] see Stains and Staining Methods, under stain … Medical dictionary
Romanowsky (Romanovsky) stain — the prototype of the many eosinâ€“polychrome methylene blue stains for blood smears and malarial parasites, including Giemsa stain, Leishman stain, and Wright stain … Medical dictionary
Romanowsky — Dimitri L., Russian physician, 1861–1921. See R. blood stain … Medical dictionary
Romanowsky (Romanovsky) method — see Stains and Staining Methods, under stain … Medical dictionary
Wright's stain — is a histologic stain that facilitates the differentiation of blood cell types. It is used primarily to stain peripheral blood smears and bone marrow aspirates which are examined under a light microscope. In cytogenetics it is used to stain… … Wikipedia
Leishman stain — Leishman s stain, also Leishman stain, is used in microscopy for staining blood smears. It provides excellent stain quality. It is generally used to differentiate and identify leucocytes, malaria parasites, and trypanosomas. It is based on a… … Wikipedia
Field's stain — is a histological method for staining of blood smears. It is used for staining thin blood films in order to discover malarial parasites. Field s stain is a version of a Romanowsky stain, used for rapid processing of the specimens.Field s stain… … Wikipedia