- Selectable marker
A selectable marker is a
geneintroduced into a cell, especially a bacteriumor to cells in culture, that confers a trait suitable for artificial selection. They are a type of reporter geneused in laboratory microbiology, molecular biology, and genetic engineeringto indicate the success of a transfectionor other procedure meant to introduce foreign DNAinto a cell. Selectable markers are often antibiotic resistancegenes; bacteria that have been subjected to a procedure to introduce foreign DNA are grown on a medium containing an antibiotic, and those bacterial colonies that can grow have successfully taken up and expressed the introduced genetic material.
An alternative to a selectable marker is a
screenable marker, which allows the researcher to distinguish between wanted and unwanted cells.
Examples of selectable markers include:
gene[Edited By C. Arias, B.S., C.P.T.]
*Neo gene from Tn5, which confers antibiotic resistance to
geneticin[ [http://www.cellmigration.org/resource/komouse/images/mousefig2.pngCallmigration.org: Gene targeting] ]
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Selectable Marker — Als Selectable Marker wird in der Gentechnik ein Gen bezeichnet, das zusammen mit dem „gene of interest“, also dem eigentlich gewünschten Gen, in den veränderten Organismus eingebracht wird, um Individuen mit erfolgreicher Genveränderung erkennen … Deutsch Wikipedia
selectable marker — A gene whose expression allows the identification of a specific trait or gene in an organism … Glossary of Biotechnology
dominant selectable marker — A gene that allows the host cell to survive under conditions where it would otherwise die. Synonym: positive selectable marker … Glossary of Biotechnology
positive selectable marker — See: dominant selectable marker … Glossary of Biotechnology
chimeric selectable marker gene — A gene that is constructed from parts of two or more different genes and allows the host cell to survive under conditions where it would otherwise die … Glossary of Biotechnology
Marker gene — A marker gene is a gene used in molecular biology to determine if a nucleic acid sequence has been successfully inserted into an organism s DNA. There are two types of marker genes: a selectable marker and a marker for screening. Selectable… … Wikipedia
marker gene — Gene that confers some readily detectable phenotype on cells carrying the gene, either in culture, or in transgenic or chimeric organisms. Gene could be an enzymic reporter gene, a selectable marker conferring antibiotic resistance, or a cell… … Dictionary of molecular biology
Marker assisted selection — or marker aided selection (MAS) is a process whereby a marker (morphological, biochemical or one based on DNA/RNA variation) is used for indirect selection of a genetic determinant or determinants of a trait of interest (i.e. productivity,… … Wikipedia
Scorable Marker — Als Scorable Marker wird in der Gentechnik ein Gen bezeichnet, das zusammen mit dem „gene of interest“, also dem eigentlich gewünschten Gen, in den veränderten Organismus eingebracht wird, um Individuen mit erfolgreicher Genveränderung erkennen… … Deutsch Wikipedia
Delitto perfetto — is a genetic technique for in vivo site directed mutagenesis in yeast. This name is an Italian term for perfect deletion and is also an idiom for perfect murder. The name refers to the ability of the technique to create desired genetic changes… … Wikipedia