proteinsthat contain a nucleic acidderivative of riboflavin: the flavin adenine dinucleotide(FAD) or flavin mononucleotide(FMN).
Flavoproteins are involved in a wide array of biological processes, including, but by no means limited to,
bioluminescence, removal of radicals contributing to oxidative stress, photosynthesis, DNA repair, and apoptosis. The spectroscopic properties of the flavincofactor make it a natural reporter for changes occurring within the active site; this makes flavoproteins one of the most-studied enzyme families.
The first mention of a flavoprotein in the scientific literature dates back to 1879, when the work on the composition of cow’s milk resulted in the isolation of a bright-yellow
pigment, that we now know as flavin, but termed lactochrome at the time. By the early 1930s, this same pigmenthad been isolated from a range of sources, and recognised as a component of the vitamin B complex. Its structure was determined almost simultaneously by two groups in 1934, and given the name riboflavin, derived from the ribityl side chain and yellow colour of the conjugated ring system.Massey, V. (2000) The chemical and biological versatility of riboflavin, Biochem. Soc. Trans. 28, 283-296.]
The first evidence for the requirement of
flavinas an enzyme cofactorcame in 1935. Hugo Theorell and coworkers showed that a bright-yellow-coloured yeast protein, identified previously as essential for cellular respiration, could be separated into apoproteinand a bright-yellow pigment. Neither apoproteinnor pigmentalone could catalyse the oxidationof NADH, but mixing of the two restored the enzymeactivity. However, replacing the isolated pigmentwith riboflavindid not restore enzymeactivity, despite their being indistinguishable under spectroscopy. This led to the discovery that the proteinstudied required not riboflavinbut flavin mononucleotideto be catalytically active ] [Theorell, H. (1935) Preparation in pure state of the effect group of yellow enzymes, Biochemische Zeitschrift 275, 344-346.]
Similar experiments with
D-amino acid oxidase[Warburg, O., and Christian, W. (1938) Isolation of the prosthetic group of the amino acid oxydase, Biochemische Zeitschrift 298, 150-168.] led to the identification of flavin adenine dinucleotide(FAD) as a second form of flavinutilised by enzymes. [Christie, S. M. H., Kenner, G. W., and Todd, A. R. (1954) NUCLEOTIDES .25. A SYNTHESIS OF FLAVIN ADENINE DINUCLEOTIDE, Journal of the Chemical Society, 46-52.]
* The menu "science" of the program [http://3d-alignment.eu/ STRAP] provides A comprehensive collection of all flavo-proteins with known 3D-structure. It compares the protein structures to elucidate phylogenetic relationships.
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