IAEDANS

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ImageFile=IAEDANS.svg
ImageSize=200px
IUPACName=5- [2- [(2-Iodo-1-oxoethyl)amino] ethylamino] -1-naphthalenesulfonic acid
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Section1=Chembox Identifiers
CASNo=36930-63-9
PubChem=91621
SMILES=C1=CC2=C(C=CC=C2S(=O)(=O)O)C(=C1)NCCNC(=O)CI
Section2=Chembox Properties
Formula=C₁₄H₁₅IN₂O₄S
MolarMass=434.25 g/mol
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Section3=Chembox Hazards
MainHazards=
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IAEDANS is an organic fluorophore (fluorescent molecule). It stands for 5-({2- [(iodoacetyl)amino] ethyl}amino)naphthalene-1-sulfonic acid. It is widely used as a marker in fluorescence spectroscopy.1,5-IAEDANS has a peak excitation wavelength of 396 nm and a peak emission wavelength of 490 nm. The extinction coefficient of the dye is 5700. It is soluble in dimethylformamide (DMF) above pH 6 and reacts primarily with thiols.

The absorption spectrum IAEDANS overlaps well with the emission spectrum of Tryptophan, making it useful as an acceptor in FRET experiments. It can also be used as a resonance energy donor to fluorophores such as fluorescein, Alexa Fluor 488, Oregon Green, and BODIPY FL.