Alphavirus


Alphavirus

Taxobox | color=violet
name = "Alphavirus"



virus_group = iv
familia = "Togaviridae"
genus = "Alphavirus"
In biology and immunology, an alphavirus belongs to the group IV Togaviridae family of viruses, according to the system of classification based on viral genome composition introduced by David Baltimore in 1971. Alphaviruses, like all other group IV viruses have a positive sense single stranded RNA genome. There are 27 alphaviruses, able to infect various vertebrates such as humans, rodents, birds, and larger mammals such as horses as well as invertebrates. Transmission between species and individuals occurs via mosquitoes making the alphaviruses a contributor to the collection of Arboviruses – or Arthropod Borne Viruses. Alphaviruses particles are enveloped have a 70nm diameter, tend to be spherical (although slightly pleomorphic), and have a 40nm isometric nucleocapsid.

Genome

The genome of alphaviruses consists of a single stranded positive sense RNA. The total genome length ranges between 11,000 and 12,000 nucleotides, and has a 5’ cap, and 3’ poly-A tail. There are two open reading frames (ORF’s) in the genome, non-structural and structural. The first is non structural and encodes proteins for transcription and replication of viral RNA, and the second encodes four structural proteins: Capsid protein C, Envelope glycoprotein E1, Envelope glycoprotein E2, and Envelope glycoprotein E3. The expression of these proteins and replication of the viral genome all takes place in the cytoplasm of the host cells.

Pathogenesis and immune response

There are many alphaviruses distributed around the world with the ability to cause human disease. Infectious arthritis, encephalitis, rashes and fever being the most commonly observed. Larger mammals such as humans and horses are usually dead-end hosts or play a minor role in viral transmission, however in the case of Venezuelan equine encephalitis the virus is mainly amplified in horses. In most other cases the virus is maintained in nature in mosquitoes, rodents and birds.

Alphavirus infections are spread by insect vectors such as mosquitoes. Once a human is bit by the infected mosquito, the virus can gain entry into the bloodstream, causing viremia. The alphavirus can also get into the CNS where it is able to grow and multiply within the neurones. This can lead to encephalitis, which can be fatal.

When an individual is infected with this particular virus, its immune system can play a role in clearing away the virus particles. Alphaviruses are able to cause the production of interferons. Antibodies and T cells are also involved. The neutralising antibodies also play an important role to prevent further infection and spread.

Diagnosis, prevention, and control

Diagnoses is based on clinical samples from which the virus can be easily isolated and identified. There are no alphavirus vaccines currently available. Vector control with repellents, protective clothing, breeding site destruction, and spraying are the preventive measures of choice.

Research

Alphaviruses are of interest to gene therapy researchers, in particular the Ross River virus, Sindbis virus, Semliki Forest virus, and Venezuelan Equine Encephalitis virus have all been used to develop viral vectors for gene delivery. Of particular interest are the chimeric viruses that may be formed with alphaviral envelopes and retroviral capsids. Such chimeras are termed pseudotyped viruses. Alphaviral envelope pseudotypes of retroviruses or lentiviruses are able to integrate the genes that they carry into the expansive range of potential host cells that are recognized and infected by the alphaviral envelope proteins E2 and E1. The stable integration of viral genes is mediated by the retroviral interiors of these vectors. There are limitations to the use of alphaviruses in the field of gene therapy due to their lack of targeting, however, through the introduction of variable antibody domains in a non-conserved loop in the structure of E2, specific populations of cells have been targeted. Furthermore, the use of whole alphaviruses for gene therapy is of limited efficacy both because several internal alphaviral proteins are involved in the induction of apoptosis upon infection and also because the alphaviral capsid mediates only the transient introduction of mRNA into host cells. Neither of these limitations extend to alphaviral envelope pseudotypes of retroviruses or lentiviruses. However, the expression of Sindbis virus envelopes may lead to apoptosis, and their introduction into host cells upon infection by Sindbis virus envelope pseudotyped retroviruses may also lead to cell death. The toxicity of Sindbis viral envelopes may be the cause of the very low production titers realized from packaging cells constructed to produce Sindbis pseudotypes. Another branch of research involving alphaviruses is in vaccination. Alphaviruses are apt to be engineered to create replicon vectors which efficiently induce humoral and T-cell immune responses. They could therefore be used to vaccinate against viral, bacterial, protozoan, and tumor antigens.

ee also

*Chikungunya
*Semliki forest virus
*Sindbis virus

Sources

*http://virology-online.com/viruses/Arboviruses2.htm
*http://www.ncbi.nlm.nih.gov/ICTVdb/ICTVdB/73010000.htm
*Alphavirus vectors: from protein production to gene therapy, C Smerdou & P Liljestrom, Gene Therapy and Regulation Vol 1 No 1 2000 pp. 33-63
*Alphavirus vectors and vaccination, J O Rayner et al, Reviews in Medical Virology vol 12 issue 5 pp 279-296
*http://ep.physoc.org/cgi/content/full/90/1/45
*http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mmed.chapter.2894
*http://www.ncbi.nlm.nih.gov/books/bv.fcgi?rid=mmed.section.2918


Wikimedia Foundation. 2010.

Look at other dictionaries:

  • Alphavirus — Systematik Reich: Viren Ordnung: nicht klass …   Deutsch Wikipedia

  • Alphavirus — sp …   Wikipédia en Français

  • alphavirus — noun Any virus of the genus Alphavirus …   Wiktionary

  • Alphavirus — Genus of the Togaviridae family of RNA viruses. Sindbis and Semliki Forest viruses are the best known examples …   Dictionary of molecular biology

  • Alphavirus — One of the genera of the family Togaviridae that was formerly classified as part of the “group A” arboviruses and includes the viruses that cause eastern equine, western equine, and Venezuelan encephalitis. * * * Al·pha·vi·rus al fə .vī rəs n a… …   Medical dictionary

  • alphavirus — n. any member of a genus of arboviruses transmitted by mosquitoes. Many alphaviruses can cause disease in humans and animals, including O nyong nyong fever and Ross River fever …   The new mediacal dictionary

  • alphavirus —  n.m. Type de virus …   Le dictionnaire des mots absents des autres dictionnaires

  • alphavirus — noun an arbovirus of the family Togaviridae that can cause a variety of encephalitis in horses • Hypernyms: ↑animal virus • Member Holonyms: ↑Togaviridae …   Useful english dictionary

  • group A arboviruses — Alphavirus …   Medical dictionary

  • Alphaviren — Alphavirus Systematik Reich: Viren Ordnung: nicht klassifiziert Familie: Togaviridae …   Deutsch Wikipedia