Restriction site

Restriction site

Restriction sites, or restriction recognition sites, are specific sequences of nucleotides that are recognized by restriction enzymes. The sites are generally palindromic, (because restriction enzymes usually bind as homodimers) and a particular restriction enzyme may cut the sequence between two nucleotides within its recognition site, or somewhere nearby. For example, the common restriction enzyme EcoRI recognizes the palindromic sequence GAATTC and cuts between the G and the A on both the top and bottom strands, leaving an overhang (an end-portion of a DNA strand with no attached complement) on each end, of AATT. This overhang can then be used to ligate in (see DNA ligase) a piece of DNA with a complementary overhang (another EcoRI-cut piece, for example).

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  • restriction site — restriction site. См. сайт рестрикции. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • restriction site — noun the specific sites at which a restriction enzyme will cleave DNA • Hypernyms: ↑site, ↑situation * * * Biochem. the place on a DNA molecule where a restriction enzyme acts …   Useful english dictionary

  • restriction site — Any site in DNA that can be cut by a restriction enzyme …   Dictionary of molecular biology

  • Restriction site — A sequence in DNA that can be recognized and cut by a specific restriction enzyme. * * * a base sequence in a DNA segment recognized by a particular restriction endonuclease …   Medical dictionary

  • restriction site — Biochem. the place on a DNA molecule where a restriction enzyme acts. * * * …   Universalium

  • restriction site — Synonym of recognition site …   Glossary of Biotechnology

  • Restriction enzyme — Glossary Restriction …   Wikipedia

  • Restriction map — A restriction map is a map of known restriction sites within a sequence of DNA. Restriction mapping requires the use of restriction enzymes. In molecular biology, restriction maps mdash;along with DNA DNA hybridization, and DNA or RNA sequence… …   Wikipedia

  • Restriction fragment length polymorphism — A Restriction fragment length polymorphism (or RFLP, often pronounced as rif lip ) is a variation in the DNA sequence of a genome that can be detected by breaking the DNA into pieces with restriction enzymes and analyzing the size of the… …   Wikipedia

  • Restriction enzyme — An enzyme from bacteria that can recognize specific base sequences in DNA and cut the DNA at that site (the restriction site). A restriction enzyme acts as a biochemical scissors. Also called a restriction endonuclease. A restriction enzyme is a… …   Medical dictionary