Magnet-assisted transfection

Magnet-assisted transfection

Magnet Assisted Transfection is a transfection method, which uses magnetic force to deliver DNA into target cells. Therefore, nucleic acids are first associated with magnetic nanoparticles. Then, application of magnetic force drives the nucleic acid-parcticle complexes towards and into the target cells, where the cargo is released.[1][2]

Principle of Magnet Assisted Transfection

Contents

The magnetic nanoparticles

Nanoparticles used as carriers for nucleic acids are mostly iron oxides.[3] These iron oxides can be generated by precipitation from acidic iron-salt solutions upon addition of appropriate bases. The magnetic nanoparticles have an approximately size of 100 nm and are additionally coated with biological polymers to allow loading of nucleic acids. Particles and nucleic acids form complexes by ionic interaction of the negatively charged nucleic acid and the positively charged surface of the magnetic nanoparticle.

DNA delivery to the target cells

The binding of the negatively charged nucleic acids to the positively charged iron particles occurs relatively fast. After complex formation, the loaded particles are incubated together with the target cells on a magnet plate. Exploiting the magnetic force, the iron particles are rapidly drawn towards the surface of the cell membrane. Cellular uptake occurs by either endocytosis or pinocytosis. Delivered to the target cells, the DNA is released into the cytoplasm. The magnetic particles are accumulated in endosomes and/or vacuoles. Over time, the nanoparticles are degraded and the iron enters the normal iron metabolism. Influence of cellular functions by iron particles has not been reported yet. Usually, in most cases the increased iron concentration in culture media does not lead to cytotoxic effects.

Advantages and prospects of Magnet Assisted Transfection

Magnet Assisted Transfection is a relatively new and time-saving method to introduce nucleic acids into a target cell with increased efficiency. In particular, adherent mammalian cell lines and primary cell cultures show very high transfection rates. But also suspension cells and cells from other organisms can be successfully transfected. Further, a major advantage of the method is the mild treatment of the cells. Other methods might be limited by either possible cytotoxic effects of the lipidic transfection reagent (lipofection) or simply by the directly applied force on the cells (electroporation, 20-50% dead cells). In addition, the transfection efficiency is increased in numerous cases by the directed transport in a magnetic field, especially for low amounts of nucleic acids. In contrast, methods like lipofection offer only statistical hits between cargo and cells, because of the three-dimensional motion of cells and transfection aggregates in a liquid suspension. Magnet Assisted Transfection can also be performed in the presence of serum, which is a further benefit. Currently, there are over 150 cells known to be successfully transfected. Additionally, synergistic effects in transfection efficiency can arise from the possible combination of lipofection and Magnet Assisted Transfection. In future, this technology might be also an alternative strategy to the currently used viral and non-viral vectors in gene-therapy and gene transfer.[4]

Literature

  1. ^ Bertram, J. (2006) MATra - Magnet Assisted Transfection: Combining Nanotechnology and Magnetic Forces to Improve Intracellular Delivery of Nucleic Acids. Current Pharmaceutical Biotechnology 7, 277-28
  2. ^ http://www.magnet-assisted-transfection.com/naps/naps_fr02_01.html
  3. ^ Plank, C., Schillinger, U., Scherer, F., Bergemann, C., Remy, J. S., Krötz, F., Anton, M., Lausier, J. and Rosenecker, J. (2003) Biol. Chem., 384, 737-747.
  4. ^ Davis, M. E. (2002) Curr. Opin. Biotechnol., 13(2), 128-131

Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать реферат

Look at other dictionaries:

  • Magnet Assisted Transfection — Die Magnet Assisted Transfection (engl. Magnet unterstützte Transfektion ) ist eine Transfektionsmethode, die unter Ausnutzung eines magnetischen Feldes DNA in die Zielzellen einschleust. Dabei werden eisenhaltige, magnetische Nanopartikel mit… …   Deutsch Wikipedia

  • Optical transfection — is the process of introducing nucleic acids into cells using light. Typically, a laser is focussed to a diffraction limited spot ( 1 µm diameter) using a high numerical aperture microscope objective. The plasma membrane of a cell is then… …   Wikipedia

  • Transfektion — Als Transfektion wird in der Zellbiologie das Einbringen von Fremd DNA oder RNA in eukaryotische Zellen bezeichnet.[1] Der Prozess ist ähnlich der bakteriellen Transformation, wird aber anders bezeichnet, da die Transformation in Säugetierzellen… …   Deutsch Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”