- Liquid chromatography-mass spectrometry
Infobox chemical analysis
name = Liquid chromatography-mass spectrometry
caption =Ion trap LCMS system
acronym = LCMS
Chromatography Mass spectrometry
organic molecules biomolecules
Thermo Fisher Scientific
Gas chromatography-mass spectrometry
Liquid chromatography-mass spectrometry (LC-MS) is an
analytical chemistrytechnique that combines the physical separation capabilities of liquid chromatography (or HPLC) with the mass analysis capabilities of mass spectrometry. LC-MS is a powerful technique used for many applications which has very high sensitivity and specificity. Generally its application is oriented towards the specific detection and potential identification of chemicals in the presence of other chemicals (in a complex mixture).
A major difference between traditional
HPLCand the chromatographyused in LC-MS is that in the latter case the scale is usually much smaller, both with respect to the internal diameter of the column and even more so with respect to flow rate since it scales as the square of the diameter. For a long time, 1 mm columns were typical for LC-MS work (as opposed to 4.6 mm for HPLC). More recently 300m and even 75m capillary columns have become more prevalent. At the low end of these column diameters the flow rates approach 100nL/min and are generally used with nanospraysources. [ [http://dx.doi.org/10.1002/mas.1280130504 Capillary liquid chromatography/mass spectrometry, Kenneth B. Tomer, M. Arthur Moseley, Leesa J. Deterding, Carol E. Parker, Mass Spectrometry Reviews, Vol 13, 1994, pp 431-457] ]
bore(4.6 mm) columns are used the flow is often split ~10:1. This can be beneficial by allowing the use of other techniques in tandem such as MS and UV. However splitting the flow to UV will decrease the sensitivity of spectrophotometric detectors. The Mass Spec on the other hand will give improved sensitivity at flow rates of 200 μL/min or less. This is because the analyte ions have to be vaporised (nebulized) in order to become charged.
There are a lot of mass analyzers that can be used in LC/MS. Single Quadrupole, Triple Quadrupole, Ion Trap, TOF (time of Flight) and Quadrupole-time of flight (Q-TOF).
Understandably the interface between a liquid phase technique which continuously flows liquid, and a gas phase technique carried out in a vacuum was difficult for a long time. The advent of
electrospray ionizationchanged this. The interface is most often an electrosprayion source or variant such as a nanospray source; however fast atom bombardment, thermosprayand atmospheric pressure chemical ionizationinterfaces are also used. [ [http://dx.doi.org/10.1002/mas.1280110103 Combined liquid chromatography mass spectrometry. Part III. Applications of thermospray, Patrick Arpino, Mass Spectrometry Reviews, Vol 11, 1992 pp 3-40] ] Various deposition and drying techniques have also been used such as using moving belts; however the most common of these is off-line MALDIdeposition. [Combined liquid chromatography mass spectrometry. Part I. Coupling by means of a moving belt interface, Patrick Arpino, Mass Spectrometry Reviews, Vol 8, 1989 pp 35-55] [ [http://www3.interscience.wiley.com/cgi-bin/abstract/10009479/ABSTRACT Coupling matrix-assisted laser desorption/ionization to liquid separations, Kermit K. Murray, Mass Spectrometry Reviews, Vol 16, pp 283-299] ]
LC-MS is very commonly used in
pharmacokineticstudies of pharmaceuticals. These studies tell us how quickly a drug will be cleared from the hepatic blood flow, and organs of the body. MS is used for this due to high sensitivity and exceptional specificity compared to UV (as long as the analyte can be suitably ionised), and short analysis time.
The major advantage MS has is the use of tandem MS-MS. You can program the detector to select certain ions to fragment. The process is essentially a selection technique, but is in fact more complex. The measured quantity is the sum of molecule fragments chosen by the operator. As long as there are no interferences or ion suppression, the LC separation can be quite quick. It is common now to have analysis times of 1 minute or less by MS-MS detection, compared to over 10 mins with UV detection. [Increasing Speed and Throughput When Using HPLC-MS/MS Systems for Drug Metabolism and Pharmacokinetic Screening, Y. Hsieh and W.A. Korfmacher, Current Drug Metabolism Volume 7, Number 5, 2006, Pp. 479-489] [Covey TR, Lee ED, Henion JD. 1986. High-speed liquid chromatography/tandem mass spectrometry for the determination of drugs in biological samples. Anal Chem 58:2453-2460.] [Thermospray liquid chromatography/mass spectrometry determination of drugs and their metabolites in biological fluids. Covey TR et al. Anal Chem. 1985 Feb;57(2):474-81]
LC-MS is also used in the study of
proteomicswhere again components of a complex mixture must be detected and identified in some manner. The bottom-up proteomicsLC-MS approach to proteomics generally involves protease digestion (usually Trypsin) followed by LC-MS with peptide mass fingerprintingor LC-MS/MS (tandem MS) to derive sequence of individual peptides.
LC-MS is frequently used in drug development at many different stages including Peptide Mapping, Glycoprotein Mapping, Natural Products Dereplication, Bioaffinity Screening, In Vivo Drug Screening, Metabolic Stability Screening, Metabolite Identification, Impurity Identification, Degradant Identification, Quantitative Bioanalysis, and Quality Control. [ [http://www3.interscience.wiley.com/cgi-bin/abstract/66004558/ABSTRACT LC/MS applications in drug development, Mass Spectrometry Reviews, Mike S. Lee, Edward H. Kerns, Vol 18, 1999, pp 187-279] ]
Gas chromatography-mass spectrometry
Ion mobility spectrometry-mass spectrometry
*cite book |author=Thurman, E. M.; Ferrer, Imma |title=Liquid chromatography/mass spectrometry, MS/MS and time of flight MS: analysis of emerging contaminants |publisher=American Chemical Society |location=Columbus, OH |year=2003 |pages= |isbn=0-8412-3825-1 |oclc= |doi=
*cite book |author=Ardrey, R. E.; Ardrey, Robert |title=Liquid chromatography-mass spectrometry: an introduction |publisher=J. Wiley |location=London |year=2003 |pages= |isbn=0-471-49801-7 |oclc= |doi=
*cite book |author=McMaster, Marvin C. |title=LC/MS: a practical user's guide |publisher=John Wiley |location=New York |year=2005 |pages= |isbn=0-471-65531-7 |oclc= |doi=
*cite book |author=Wilfried M.A. Niessen, Wilfried M. Niessen |title=Liquid Chromatography-Mass Spectrometry, Third Edition (Chromatographic Science) |publisher=CRC |location=Boca Raton |year= 2006 |pages= |isbn=0-8247-4082-3 |oclc= |doi=
*cite book |author=Yergey, Alfred L. |title=Liquid chromatography/mass spectrometry: techniques and applications |publisher=Plenum Press |location=New York |year=1990 |pages= |isbn=0-306-43186-6 |oclc= |doi=
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