Glucuronidase


Glucuronidase

protein
Name= glucuronidase, beta
caption=


width=
HGNCid=4696
Symbol=GUSB
AltSymbols=
EntrezGene=2990
OMIM=253220
RefSeq=NM_000181
UniProt=P08236
PDB=
ECnumber=3.2.1.31
Chromosome=7
Arm=q
Band=22
LocusSupplementaryData=

Glucuronidases are enzymes that separate glucuronic acid molecules from other molecules by cutting glycosidic bonds. They are thus classified as glycoside hydrolases that cleave glucuronides.

In eukaryotes, glucuronidase is located in lysosomes and plays an important role in recycling cellular components by cleaving glucuronide moieties from proteins. Glucuronidase exhibits both endo-glycosidase and exo-glycosidase activities, meaning that it can cleave monosaccharides from the middle of a chain or from the end.

Preparations of beta-glucuronidase from the snail "Helix pomatia" are commercially available and referred to by the trademark "Glusulase". These enzymes preparations are used to digest the ascus of the yeast "Saccharomyces cerevisiae" after sporulation.

"E. coli" is among the few bacteria that can synthezise glucuronidase, and this trait is commonly used to identify the bacterium.

Pathology

Sly syndrome is an inherited disease in which a genetic defect leads to a deficiency in glucuronidase. This results in numerous symptoms because of the accumulation of complex carbohydrates in many tissues and organs of the body.

Use as reporter gene

In molecular biology, beta-glucuronidase is used as a reporter gene to monitor gene expression. Monitoring glucuronidase activity using a so-called GUS assay allows determination of the spatial and temporal expression of the gene being monitored. The beta-glucuronidase enzyme transforms certain substrates into colored or fluorescent products which can then be observed and measured.

External links

*cite journal | author=George J.|title=Elevated serum beta-glucuronidase reflects hepatic lysosomal fragility following toxic liver injury in rats. |journal=Biochem Cell Biol. |volume=86|issue= 3 |pages= 235-243. |year= 2008 |pmid= 18523484 |doi= 10.1139/O08-038
*
* [http://www.reportergene.com/search/label/other%20reporters Updated research on reporter glucuronidase] and other reporters from Reportergene


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Look at other dictionaries:

  • Glucuronidase — Bänder /Oberflächenmodell des Dimers mit Nonaglykosid nach PDB  …   Deutsch Wikipedia

  • Glucuronidase — La β glucuronidase est une enzyme lysosomale chez l homme, qui casse les liaisons osidiques des glucuronides. Une mutation de ce gène provoque la maladie de Sly. Le gène de la β glucuronidase est utilisé en biologie moléculaire comme gène… …   Wikipédia en Français

  • glucuronidase — See: beta glucuronidase …   Glossary of Biotechnology

  • glucuronidase — ˌglükyəˈränəˌdās, āz noun ( s) Etymology: glucuronide + ase : an enzyme that hydrolyzes a glucuronide; especially : an enzyme that occurs widely (as in liver and spleen) and is active toward a beta glucuronide the physiological role of beta… …   Useful english dictionary

  • glucuronidase — noun Date: 1945 an enzyme that hydrolyzes a glucuronide; especially one that occurs widely (as in liver and spleen) and hydrolyzes the beta form of a glucuronide …   New Collegiate Dictionary

  • glucuronidase — noun Any enzyme that catalyzes the hydrolysis of a glucuronide; a glycuronidase …   Wiktionary

  • glucuronidase — gluc·uron·i·dase rän ə .dās, .dāz n an enzyme that hydrolyzes a glucuronide esp one that occurs widely (as in the liver and spleen) and hydrolyzes the beta form of a glucuronide …   Medical dictionary

  • glucuronidase — glu·cu·ron·i·dase …   English syllables

  • Béta-glucuronidase — Glucuronidase La β glucuronidase est une enzyme lysosomale chez l homme. Une mutation de ce gène provoque la maladie de Sly. Le gène de la β glucuronidase est utilisé en biologie moléculaire comme gène rapporteur sous le nom de gène GUS. Portail… …   Wikipédia en Français

  • Béta glucuronidase — Glucuronidase La β glucuronidase est une enzyme lysosomale chez l homme. Une mutation de ce gène provoque la maladie de Sly. Le gène de la β glucuronidase est utilisé en biologie moléculaire comme gène rapporteur sous le nom de gène GUS. Portail… …   Wikipédia en Français