Direct fluorescent antibody


Direct fluorescent antibody
Direct FA stained mouse brain impression smear reveals the presence of the bacterium Chlamydia psittaci. 400X.

Direct fluorescent antibody (DFA or dFA) (also known as "Direct immunofluorescence"[1]) is a laboratory test that uses antibodies tagged with fluorescent dye that can be used to detect the presence of microorganisms. This method offers straightforward detection of antigens using fluorescently labeled antigen-specific antibodies. Because detection of the antigen in a substrate of patient sample (cellular smear, fluid or patient- inoculated culture medium) is the goal, DFA is seldom quantitative.

This is the main test used to detect rabies in animals and requires the examination of brain tissue.

See also

References

  1. ^ Pober, Jordan S.; Abbas, Abul K.; Lichtman, Andrew H. (2000). Cellular and molecular immunology. Philadelphia: Saunders. ISBN 0-7216-8233-2. 

External links