- High throughput cell biology
High Throughput Cell Biology is the combination of
cell biologywith automation to address questions from the scale of a single cell to the human Genome. It permits questions to be addressed that are otherwise unattainable using conventional methods. It exploits optics, chemistry, biologyand image analysisto permit rapid, highly parallel research into how cells function, interact with each other and how pathogens exploit them in disease.
It has also been called "Omics research" - the interface between large scale biology (Genome, proteome, transcriptome), technology and researchers. High throughput cell biology has a definite focus on the cell, and methods accessing the cell such as imaging, gene expression microarrays, genome wide screening.The basic idea is to ake bench methods and do simply a very large number of them without losing their quality.
High Throughput Cell Biology —a definition
High throughput research can be defined as the automation of experiments such that large scale repetition becomes feasible. This is important because many of the questions faced by life science researchers now involve large numbers. For example, the Human Genome contains at least 21'000 genes, all of which can potentially contribute to cell function, or disease. To be able to capture an idea of how these genes interact with one another, which genes are involved in and where they are, methods that encompass from the cell to the genome are of interest.
to be doneClassical
High throughput screeningrobotics are now being tied closer to cell biology, principally using technologies such as High-content screening. High throughput cell biology dictates methods that can take routine cell biology from low scale research to the speed and accuracy that allows the entire genome to be looked at, very quickly.
It has a greater emphasis on function rather than discovery, and will have its most significant impact in exploring biology as we progress toward models of the cell as a system rather than isolated pathways.
High Throughput Cell Biology —what it is good for
High-Throughput Cell Biology- faster and faster cellular research and discovery
* Ritter, R. (2002). "The Oxford Style Manual". Oxford University Press. ISBN 0-19-860564-1
From one to many—what this offers for research
This technology allows a (very) large number of experiments to be performed, dramatically more than were possible. What is this good for?
High-Throughput cell biology and its technology
High-content screening technology is mainly based on automated digital microscopy and flow cytometry, in combination with IT-systems for the analysis and storage of the data.“High-content” or visual biology technology has two purposes, first to acquire spatially or temporally resolved information on an event and second to automatically quantify it. Spatially resolved instruments are typically automated
microscopes, and temporal resolution still requires some form of fluorescence measurement in most cases.This means that a lot of HCS instruments are ( fluorescence) microscopes that are connected to some form of image analysis package. These take care of all the steps in taking fluorescent images of cells and provide rapid, automated and unbiased assessment of experiments.
The instruments on the market are summarised here
High-content screening.Kits for high-content screening of various target proteins (e.g. p53, c-jun and NFkB) have recently become available from commercial suppliers.
Where we are
The technology can be defined as being at the same development point as the first automated
DNA sequencers in the early 1990’s. Automated DNA sequencingwas a disruptive technology when it became practical and -even if early devices had shortcomings- it enabled genome scale sequencing projects and created the field of bioinformatics. The impact of a similarly disruptive and powerful technology on molecular cell biology and translational research is hard to predict but what is clear is that it will cause a profound change in the way cell biologists research and medicines are discovered.
Timeline of the evolution of the science and technology
Please contribute with dates and key assay and image analysis technologies...
Drug discovery hit to lead
Flow cytometry High-content screening
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