- blue-white colour selection
Method for identifying bacterial clones containing plasmids with inserts. Many modern vectors have their polycloning site within a part of the LacZ gene encoding beta-galactosidase, which provides a -complementation in an appropriate mutant E. coli strain. This means that a re-ligated (empty) vector will produce blue colonies when grown on plates containing IPTG and Xgal, but colonies with a substantial insert in their plasmid\'s polycloning site are unable to produce functional beta-galactosidase, and so produce white colonies.
Dictionary of molecular biology. 2004.
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beta-galactosidase — (= b galactosidase) Enzyme (EC 188.8.131.52) encoded by the LacZ gene, that is widely used as a reporter gene, as a variety of coloured or fluorescent compounds can be produced from appropriate substrates (typically Xgal, that produces a blue colour) … Dictionary of molecular biology
Xgal — (= 5 bromo 4 chloro 3 indolyl b D galactoside) Chromogenic substrate for the enzyme beta galactosidase, yielding an intense blue colour. Very widely used in blue white colour selection of bacteria containing recombinant plasmids, and in… … Dictionary of molecular biology
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