Light-induced change in a chromophore, resulting in the loss of its absorption of light of a particular wavelength. A problem in fluorescence microscopy where prolonged illumination leads to progressive fading of the emitted light because less of the exciting wavelength is being absorbed.

Dictionary of molecular biology. 2004.

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  • Photobleaching — is the photochemical destruction of a fluorophore. In microscopy, photobleaching may complicate the observation of fluorescent molecules, since they will eventually be destroyed by the light exposure necessary to stimulate them into fluorescing.… …   Wikipedia

  • photobleaching — noun The destruction of a photochemical fluorescence by high intensity light …   Wiktionary

  • photobleaching — …   Useful english dictionary

  • Fluorescence recovery after photobleaching — (FRAP) denotes an optical technique capable of quantifying the two dimensional lateral diffusion of a molecularly thin film containing fluorescently labeled probes, or to examine single cells. This technique is very useful in biological studies… …   Wikipedia

  • Fluorescence loss in photobleaching — Fluorescence Loss in Photobleaching, or FLIP, is a technique in fluorescence microscopy which can be used to examine the movement or diffusion of molecules inside cells or membranes. Typically a cell membrane is labelled with a fluorescent dye,… …   Wikipedia

  • Fluorescence Loss in Photobleaching — Als Fluorescence Loss in Photobleaching (FLIP) bezeichnet man eine Methode der Mikrobiologie zum Nachweis der Fluidität von Biomembranen. Funktionsweise Dabei werden Oberflächenproteine auf Zellen mit fluoreszenz markierten Antikörpern… …   Deutsch Wikipedia

  • Fluorescence Recovery after Photobleaching — Prinzip von FRAP FRAP (Fluorescence Recovery after Photobleaching) bezeichnet eine Methode aus der Mikrobiologie und Biophysik, die zur Messung der Diffusionsgeschwindigkeiten in Zellen und dünnen Flüssigkeitsfilmen dient. Sie kann z. B. zum… …   Deutsch Wikipedia

  • fluorescence recovery after photobleaching — (= FRAP) Many fluorochromes are bleached by exposure to exciting light. If, for example, the cell surface is labelled with a fluorescent probe and an area bleached by laser illumination, then the bleached patch that starts off as a dark area will …   Dictionary of molecular biology

  • Förster resonance energy transfer — (abbreviated FRET), also known as Fluoresence resonance energy transfer or resonance energy transfer (RET), describes an energy transfer mechanism between two chromophores. A donor chromophore in its excited state can transfer energy by a… …   Wikipedia

  • Fluorescence microscope — An upright fluorescence microscope (Olympus BX61) with the fluorescent filter cube turret above the objective lenses, coupled with a digital camera. A fluorescence microscope is an optical microscope used to study properties of organic or… …   Wikipedia

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