PAP technique

PAP technique
(1) Colloquial abbreviation for Papanicolaou\'s stain,
(2) Peroxidase-antiperoxidase method for obtaining an enhanced peroxidase reaction to indicate antibody binding to antigen. In the first stage the material, eg. a section, is reacted with a specific antiserum (say rat) against the antigen. In the next stage a large excess of (say) rabbit anti-rat immunoglobulin is applied so that only one of the binding sites is bound to the first antibody. Then a rat antiperoxidase antiserum is bound to the second antibody\'s unfilled sites and finally peroxidase is added and binds to the third antiserum before the peroxidase is used to develop a colour reaction.

Dictionary of molecular biology. 2004.

Look at other dictionaries:

  • PAP technique — peroxidase antiperoxidase t …   Medical dictionary

  • Pap test — A screening test for cervical cancer based on the examination under the microscope of cells collected from the cervix, smeared on a slide and specially stained to reveal premalignant (before cancer) and malignant (cancer) changes as well as… …   Medical dictionary

  • Pap test — Intervention ICD 9 CM 795.00 …   Wikipedia

  • Pap smear — pap n a method or a test based on it for the early detection of cancer esp. of the uterine cervix that involves staining exfoliated cells by a special technique which differentiates diseased tissue called also Papanicolaou smear, Papanicolaou… …   Medical dictionary

  • Pap test — 1963, short for Papanicolaou (1947) in reference to George Nicholas Papanicolaou (1883 1962), Greek born U.S. anatomist who developed the technique of examining secreted cells to test for cancer …   Etymology dictionary

  • Pap test, ThinPrep — A modified Pap test technique designed to reduce some of the technical problems inherent in the traditional type of Pap smear. In the traditional Pap test, cell samples are obtained from the vagina, cervix, and cervical canal and spread on a… …   Medical dictionary

  • PAP — Acronym for peroxidase antiperoxidase complex. Abbreviation for 3′ phosphoadenosine 5′ phosphate. See P. technique. * * * pancreatitis associated protein; Papanicolaou [test]; papaverine; passive aggressive personality; password authentication… …   Medical dictionary

  • Pap smear — or Papanicolaou smear Sample of cells from the vagina and cervix of the uterus for laboratory staining and examination to detect genital herpes and early stage cancer, especially of the cervix. Developed by the Greek born U.S. physician George… …   Universalium

  • pap — A food of soft consistency, like that of breadcrumbs soaked in milk or water. * * * pancreatitis associated protein; Papanicolaou [test]; papaverine; passive aggressive personality; password authentication protocol; patient assessment program;… …   Medical dictionary

  • Pap Smear —    The Pap test, a stain analysis technique for identifying atypical cells and malignancy in cervical tissue smears, was the invention of George Nicholas Papanicolaou (1883 1962). In the test he devised, a small spatula is inserted into the… …   Dictionary of eponyms

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”

We are using cookies for the best presentation of our site. Continuing to use this site, you agree with this.