- chromosome walking
A procedure to find and sequence a gene whose approximate position in a chromosome is known by classical genetic linkage studies. Starting with the known sequence of a gene shown by classical genetics to be near to the novel gene, new clones are picked from a genomic library by hybridization with a short probe generated from the appropriate end of the known sequence. The new clones are then sequenced, new probes generated, and the process repeated until the gene of interest is reached.
Dictionary of molecular biology. 2004.
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chromosome walking — chromosome walking. См. прогулка по хромосоме. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
chromosome walking — in molecular genetics, the sequential isolation of clones carrying overlapping DNA sequences so that the isolation â€œwalksâ€ along part of a chromosome; it is important for isolation of a specific locus for which there is no probe but which is… … Medical dictionary
chromosome walking — A strategy for mapping or sequencing a chromosome segment and for positional cloning. Large restriction fragments (or BAC clones) are generated and, after probing, a single starting point is identified. New probes are synthesized complementary to … Glossary of Biotechnology
Chromosome jumping — is a tool of molecular biology that is used in the physical mapping of genomes. It is related to several other tools used for the same purpose, including chromosome walking. Chromosome jumping is used to bypass regions difficult to clone, such as … Wikipedia
Chromosome landing — The genetic technique of chromosome landing is a method of cloning of a gene of interest from a clone library. It is based on the principle that the expected average between marker distances can be smaller than the average insert length of a… … Wikipedia
walking — See: chromosome walking; primer walking … Glossary of Biotechnology
chromosome landing — An alteRNAtive to chromosome walking for positional cloning. Clones of genomic DNA are fragmented so as to include both the target gene and a closely linked marker and are screened to select ( land on ) those clones that contain the target gene … Glossary of Biotechnology
Chromosome — A visible carrier of the genetic information. The 3 billion bp (base pairs) in the human genome are organized into 24 distinct, physically separate microscopic units called chromosomes. All genes are arranged linearly along the chromosomes. The… … Medical dictionary
Primer walking — is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases. Such fragments are too long to be sequenced in a single sequence read using the chain termination method. This method works by dividing the long sequence… … Wikipedia
Saut sur le chromosome — Le saut sur le chromosome est une technique de biologie moléculaire utilisée pour établir une carte physique d un génome. Cette technique est utilisée avec d autres méthode, comme la marche sur le chromosome. Le saut sur le chromosome est utilisé … Wikipédia en Français