DDX20

DEAD (Asp-Glu-Ala-Asp) box polypeptide 20
PDB rendering based on 2oxc.
Available structures PDB 2OXC, 3B7G
Identifiers
Symbols	DDX20; DKFZp434H052; DP103; GEMIN3
External IDs	OMIM: 606168 MGI: 1858415 HomoloGene: 5214 GeneCards: DDX20 Gene
EC number	3.6.4.13
Gene Ontology Molecular function • nucleotide binding • DNA binding • ATP-dependent RNA helicase activity • helicase activity • protein binding • ATP binding • hydrolase activity Cellular component • nucleus • nucleoplasm • spliceosomal complex • cytoplasm • cytosol • cytosol • cytoskeleton • Cajal body • transcriptional repressor complex Biological process • negative regulation of transcription from RNA polymerase II promoter • assembly of spliceosomal tri-snRNP • spliceosomal snRNP assembly • RNA processing • mRNA processing • induction of apoptosis • RNA splicing • RNA metabolic process • ncRNA metabolic process • positive regulation of apoptosis Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	11218	53975
Ensembl	ENSG00000064703	ENSMUSG00000027905
UniProt	Q9UHI6	Q059Z6
RefSeq (mRNA)	NM_007204	NM_017397.3
RefSeq (protein)	NP_009135	NP_059093.3
Location (UCSC)	Chr 1: 112.3 – 112.31 Mb	Chr 3: 105.48 – 105.49 Mb
PubMed search	[1]	[2]

Probable ATP-dependent RNA helicase DDX20 is an enzyme that in humans is encoded by the DDX20 gene.^[1][2]

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, which has an ATPase activity and is a component of the survival of motor neurons (SMN) complex. This protein interacts directly with SMN, the spinal muscular atrophy gene product, and may play a catalytic role in the function of the SMN complex on RNPs.^[2]

Interactions

DDX20 has been shown to interact with LSM2,^[3] Small nuclear ribonucleoprotein polypeptide F,^[3] SNRPG,^[3] GEMIN4,^[4][5][6] Survival of motor neuron protein-interacting protein 1,^[6][3] SMN1,^[4][7][6][3] Small nuclear ribonucleoprotein D1,^[3] SNRPB,^[3] GEMIN5,^[8][6] EIF2C2,^[9][6] Small nuclear ribonucleoprotein D2,^[3] SNRPD3^[3] and Small nuclear ribonucleoprotein polypeptide E.^[3]

References

1. ^ Grundhoff AT, Kremmer E, Tureci O, Glieden A, Gindorf C, Atz J, Mueller-Lantzsch N, Schubach WH, Grasser FA (Jul 1999). "Characterization of DP103, a novel DEAD box protein that binds to the Epstein-Barr virus nuclear proteins EBNA2 and EBNA3C". J Biol Chem 274 (27): 19136–44. doi:10.1074/jbc.274.27.19136. PMID 10383418. 
2. ^ ^{a b} "Entrez Gene: DDX20 DEAD (Asp-Glu-Ala-Asp) box polypeptide 20". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=11218. 
3. ^ ^{a b c d e f g h i j} Charroux, B; Pellizzoni L, Perkinson R A, Shevchenko A, Mann M, Dreyfuss G (Dec. 1999). "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems". J. Cell Biol. (UNITED STATES) 147 (6): 1181–94. doi:10.1083/jcb.147.6.1181. ISSN 0021-9525. PMC 2168095. PMID 10601333. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2168095. 
4. ^ ^{a b} Carnegie, Graeme K; Sleeman Judith E, Morrice Nick, Hastie C James, Peggie Mark W, Philp Amanda, Lamond Angus I, Cohen Patricia T W (May. 2003). "Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs". J. Cell. Sci. (England) 116 (Pt 10): 1905–13. doi:10.1242/jcs.00409. ISSN 0021-9533. PMID 12668731. 
5. ^ Charroux, B; Pellizzoni L, Perkinson R A, Yong J, Shevchenko A, Mann M, Dreyfuss G (Mar. 2000). "Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli". J. Cell Biol. (UNITED STATES) 148 (6): 1177–86. doi:10.1083/jcb.148.6.1177. ISSN 0021-9525. PMC 2174312. PMID 10725331. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2174312. 
6. ^ ^{a b c d e} Mourelatos, Zissimos; Dostie Josée, Paushkin Sergey, Sharma Anup, Charroux Bernard, Abel Linda, Rappsilber Juri, Mann Matthias, Dreyfuss Gideon (Mar. 2002). "miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs". Genes Dev. (United States) 16 (6): 720–8. doi:10.1101/gad.974702. ISSN 0890-9369. PMC 155365. PMID 11914277. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=155365. 
7. ^ Meister, G; Bühler D, Laggerbauer B, Zobawa M, Lottspeich F, Fischer U (Aug. 2000). "Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins". Hum. Mol. Genet. (ENGLAND) 9 (13): 1977–86. doi:10.1093/hmg/9.13.1977. ISSN 0964-6906. PMID 10942426. 
8. ^ Gubitz, Amelie K; Mourelatos Zissimos, Abel Linda, Rappsilber Juri, Mann Matthias, Dreyfuss Gideon (Feb. 2002). "Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins". J. Biol. Chem. (United States) 277 (7): 5631–6. doi:10.1074/jbc.M109448200. ISSN 0021-9258. PMID 11714716. 
9. ^ Nelson, Peter T; Hatzigeorgiou Artemis G, Mourelatos Zissimos (Mar. 2004). "miRNP:mRNA association in polyribosomes in a human neuronal cell line". RNA (United States) 10 (3): 387–94. doi:10.1261/rna.5181104. ISSN 1355-8382. PMC 1370934. PMID 14970384. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370934. 

Further reading

• Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction.". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474. 
• Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing.". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139146. 
• Charroux B, Pellizzoni L, Perkinson RA, et al. (2000). "Gemin3: A novel DEAD box protein that interacts with SMN, the spinal muscular atrophy gene product, and is a component of gems.". J. Cell Biol. 147 (6): 1181–94. doi:10.1083/jcb.147.6.1181. PMC 2168095. PMID 10601333. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2168095. 
• Charroux B, Pellizzoni L, Perkinson RA, et al. (2000). "Gemin4. A novel component of the SMN complex that is found in both gems and nucleoli.". J. Cell Biol. 148 (6): 1177–86. doi:10.1083/jcb.148.6.1177. PMC 2174312. PMID 10725331. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2174312. 
• Meister G, Bühler D, Laggerbauer B, et al. (2000). "Characterization of a nuclear 20S complex containing the survival of motor neurons (SMN) protein and a specific subset of spliceosomal Sm proteins.". Hum. Mol. Genet. 9 (13): 1977–86. doi:10.1093/hmg/9.13.1977. PMID 10942426. 
• Pellizzoni L, Charroux B, Rappsilber J, et al. (2001). "A functional interaction between the survival motor neuron complex and RNA polymerase II.". J. Cell Biol. 152 (1): 75–85. doi:10.1083/jcb.152.1.75. PMC 2193649. PMID 11149922. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2193649. 
• Friesen WJ, Paushkin S, Wyce A, et al. (2001). "The methylosome, a 20S complex containing JBP1 and pICln, produces dimethylarginine-modified Sm proteins.". Mol. Cell. Biol. 21 (24): 8289–300. doi:10.1128/MCB.21.24.8289-8300.2001. PMC 99994. PMID 11713266. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=99994. 
• Gubitz AK, Mourelatos Z, Abel L, et al. (2002). "Gemin5, a novel WD repeat protein component of the SMN complex that binds Sm proteins.". J. Biol. Chem. 277 (7): 5631–6. doi:10.1074/jbc.M109448200. PMID 11714716. 
• Pellizzoni L, Baccon J, Rappsilber J, et al. (2002). "Purification of native survival of motor neurons complexes and identification of Gemin6 as a novel component.". J. Biol. Chem. 277 (9): 7540–5. doi:10.1074/jbc.M110141200. PMID 11748230. 
• Mourelatos Z, Dostie J, Paushkin S, et al. (2002). "miRNPs: a novel class of ribonucleoproteins containing numerous microRNAs.". Genes Dev. 16 (6): 720–8. doi:10.1101/gad.974702. PMC 155365. PMID 11914277. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=155365. 
• Klappacher GW, Lunyak VV, Sykes DB, et al. (2002). "An induced Ets repressor complex regulates growth arrest during terminal macrophage differentiation.". Cell 109 (2): 169–80. doi:10.1016/S0092-8674(02)00714-6. PMID 12007404. 
• Baccon J, Pellizzoni L, Rappsilber J, et al. (2002). "Identification and characterization of Gemin7, a novel component of the survival of motor neuron complex.". J. Biol. Chem. 277 (35): 31957–62. doi:10.1074/jbc.M203478200. PMID 12065586. 
• Narayanan U, Ospina JK, Frey MR, et al. (2003). "SMN, the spinal muscular atrophy protein, forms a pre-import snRNP complex with snurportin1 and importin beta.". Hum. Mol. Genet. 11 (15): 1785–95. doi:10.1093/hmg/11.15.1785. PMC 1630493. PMID 12095920. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1630493. 
• Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences.". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Carnegie GK, Sleeman JE, Morrice N, et al. (2004). "Protein phosphatase 4 interacts with the Survival of Motor Neurons complex and enhances the temporal localisation of snRNPs.". J. Cell. Sci. 116 (Pt 10): 1905–13. doi:10.1242/jcs.00409. PMID 12668731. 
• Lehner B, Semple JI, Brown SE, et al. (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region.". Genomics 83 (1): 153–67. doi:10.1016/S0888-7543(03)00235-0. PMID 14667819. 
• Ota T, Suzuki Y, Nishikawa T, et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs.". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. 
• Nelson PT, Hatzigeorgiou AG, Mourelatos Z (2004). "miRNP:mRNA association in polyribosomes in a human neuronal cell line.". RNA 10 (3): 387–94. doi:10.1261/rna.5181104. PMC 1370934. PMID 14970384. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1370934. 
• Beausoleil SA, Jedrychowski M, Schwartz D, et al. (2004). "Large-scale characterization of HeLa cell nuclear phosphoproteins.". Proc. Natl. Acad. Sci. U.S.A. 101 (33): 12130–5. doi:10.1073/pnas.0404720101. PMC 514446. PMID 15302935. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=514446.