NEDD9

Neural precursor cell expressed, developmentally down-regulated 9
Identifiers
Symbols	NEDD9; CAS-L; CAS2; CASL; CASS2; HEF1; dJ49G10.2; dJ761I2.1
External IDs	OMIM: 602265 MGI: 97302 HomoloGene: 4669 GeneCards: NEDD9 Gene
Gene Ontology Molecular function • protein binding Cellular component • spindle pole • nucleus • cytoplasm • Golgi apparatus • spindle • cytoskeleton • focal adhesion • cell cortex • lamellipodium • cell junction Biological process • cytoskeleton organization • cell cycle • mitosis • cell adhesion • signal transduction • integrin-mediated signaling pathway • regulation of growth • actin filament bundle assembly • cell division Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	4739	18003
Ensembl	ENSG00000111859	ENSMUSG00000021365
UniProt	Q14511	O35177
RefSeq (mRNA)	NM_001142393.1	NM_017464
RefSeq (protein)	NP_001135865.1	NP_059492
Location (UCSC)	Chr 6: 11.18 – 11.38 Mb	Chr 13: 41.41 – 41.58 Mb
PubMed search	[1]	[2]

Enhancer of filamentation 1 is a protein that in humans is encoded by the NEDD9 gene.^[1]

Interactions

NEDD9 has been shown to interact with ID2,^[2] LYN,^[3] NCK1,^[4] MICAL1,^[5] CDH1,^[6] Abl gene,^[4][7] PTK2,^[7] CRKL,^{[3][8][9][10]} CRK^[4][11] and Mothers against decapentaplegic homolog 3.^[6][12][13]

References

1. ^ "Entrez Gene: NEDD9 neural precursor cell expressed, developmentally down-regulated 9". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=4739. 
2. ^ Law, S F; Zhang Y Z, Fashena S J, Toby G, Estojak J, Golemis E A (Oct. 1999). "Dimerization of the docking/adaptor protein HEF1 via a carboxy-terminal helix-loop-helix domain". Exp. Cell Res. (UNITED STATES) 252 (1): 224–35. doi:10.1006/excr.1999.4609. ISSN 0014-4827. PMID 10502414. 
3. ^ ^{a b} Manié, S N; Beck A R, Astier A, Law S F, Canty T, Hirai H, Druker B J, Avraham H, Haghayeghi N, Sattler M, Salgia R, Griffin J D, Golemis E A, Freedman A S (Feb. 1997). "Involvement of p130(Cas) and p105(HEF1), a novel Cas-like docking protein, in a cytoskeleton-dependent signaling pathway initiated by ligation of integrin or antigen receptor on human B cells". J. Biol. Chem. (UNITED STATES) 272 (7): 4230–6. doi:10.1074/jbc.272.7.4230. ISSN 0021-9258. PMID 9020138. 
4. ^ ^{a b c} Minegishi, M; Tachibana K, Sato T, Iwata S, Nojima Y, Morimoto C (Oct. 1996). "Structure and function of Cas-L, a 105-kD Crk-associated substrate-related protein that is involved in beta 1 integrin-mediated signaling in lymphocytes". J. Exp. Med. (UNITED STATES) 184 (4): 1365–75. doi:10.1084/jem.184.4.1365. ISSN 0022-1007. PMC 2192828. PMID 8879209. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2192828. 
5. ^ Suzuki, Takahiro; Nakamoto Tetsuya, Ogawa Seishi, Seo Sachiko, Matsumura Tomoko, Tachibana Kouichi, Morimoto Chikao, Hirai Hisamaru (Apr. 2002). "MICAL, a novel CasL interacting molecule, associates with vimentin". J. Biol. Chem. (United States) 277 (17): 14933–41. doi:10.1074/jbc.M111842200. ISSN 0021-9258. PMID 11827972. 
6. ^ ^{a b} Nourry, Claire; Maksumova Lola, Pang Mona, Liu Xiaohong, Wang Tongwen (May. 2004). "Direct interaction between Smad3, APC10, CDH1 and HEF1 in proteasomal degradation of HEF1". BMC Cell Biol. (England) 5: 20. doi:10.1186/1471-2121-5-20. PMC 420458. PMID 15144564. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=420458. 
7. ^ ^{a b} Law, S F; Estojak J, Wang B, Mysliwiec T, Kruh G, Golemis E A (Jul. 1996). "Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae". Mol. Cell. Biol. (UNITED STATES) 16 (7): 3327–37. ISSN 0270-7306. PMC 231327. PMID 8668148. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=231327. 
8. ^ Kyono, W T; de Jong R, Park R K, Liu Y, Heisterkamp N, Groffen J, Durden D L (Nov. 1998). "Differential interaction of Crkl with Cbl or C3G, Hef-1, and gamma subunit immunoreceptor tyrosine-based activation motif in signaling of myeloid high affinity Fc receptor for IgG (Fc gamma RI)". J. Immunol. (UNITED STATES) 161 (10): 5555–63. ISSN 0022-1767. PMID 9820532. 
9. ^ Astier, A; Manié S N, Law S F, Canty T, Haghayghi N, Druker B J, Salgia R, Golemis E A, Freedman A S (Dec. 1997). "Association of the Cas-like molecule HEF1 with CrkL following integrin and antigen receptor signaling in human B-cells: potential relevance to neoplastic lymphohematopoietic cells". Leuk. Lymphoma (SWITZERLAND) 28 (1–2): 65–72. doi:10.3109/10428199709058332. ISSN 1042-8194. PMID 9498705. 
10. ^ Sattler, M; Salgia R, Shrikhande G, Verma S, Uemura N, Law S F, Golemis E A, Griffin J D (May. 1997). "Differential signaling after beta1 integrin ligation is mediated through binding of CRKL to p120(CBL) and p110(HEF1)". J. Biol. Chem. (UNITED STATES) 272 (22): 14320–6. doi:10.1074/jbc.272.22.14320. ISSN 0021-9258. PMID 9162067. 
11. ^ Ohashi, Y; Tachibana K, Kamiguchi K, Fujita H, Morimoto C (Mar. 1998). "T cell receptor-mediated tyrosine phosphorylation of Cas-L, a 105-kDa Crk-associated substrate-related protein, and its association of Crk and C3G". J. Biol. Chem. (UNITED STATES) 273 (11): 6446–51. doi:10.1074/jbc.273.11.6446. ISSN 0021-9258. PMID 9497377. 
12. ^ Feng, Libing; Guedes Susana, Wang Tongwen (Jul. 2004). "Atrophin-1-interacting protein 4/human Itch is a ubiquitin E3 ligase for human enhancer of filamentation 1 in transforming growth factor-beta signaling pathways". J. Biol. Chem. (United States) 279 (28): 29681–90. doi:10.1074/jbc.M403221200. ISSN 0021-9258. PMID 15051726. 
13. ^ Liu, X; Elia A E, Law S F, Golemis E A, Farley J, Wang T (Dec. 2000). "A novel ability of Smad3 to regulate proteasomal degradation of a Cas family member HEF1". EMBO J. (ENGLAND) 19 (24): 6759–69. doi:10.1093/emboj/19.24.6759. ISSN 0261-4189. PMC 305889. PMID 11118211. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=305889. 

Further reading

• Iwata S, Ohashi Y, Kamiguchi K, Morimoto C (2000). "Beta 1-integrin-mediated cell signaling in T lymphocytes". J. Dermatol. Sci. 23 (2): 75–86. doi:10.1016/S0923-1811(99)00096-1. PMID 10808124. 
• O'Neill GM, Seo S, Serebriiskii IG, et al. (2007). "A new central scaffold for metastasis: parsing HEF1/Cas-L/NEDD9". Cancer Res. 67 (19): 8975–9. doi:10.1158/0008-5472.CAN-07-1328. PMC 2637184. PMID 17908996. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2637184. 
• Law SF, Estojak J, Wang B, et al. (1996). "Human enhancer of filamentation 1, a novel p130cas-like docking protein, associates with focal adhesion kinase and induces pseudohyphal growth in Saccharomyces cerevisiae". Mol. Cell. Biol. 16 (7): 3327–37. PMC 231327. PMID 8668148. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=231327. 
• Minegishi M, Tachibana K, Sato T, et al. (1996). "Structure and function of Cas-L, a 105-kD Crk-associated substrate-related protein that is involved in beta 1 integrin-mediated signaling in lymphocytes". J. Exp. Med. 184 (4): 1365–75. doi:10.1084/jem.184.4.1365. PMC 2192828. PMID 8879209. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2192828. 
• Manié SN, Beck AR, Astier A, et al. (1997). "Involvement of p130(Cas) and p105(HEF1), a novel Cas-like docking protein, in a cytoskeleton-dependent signaling pathway initiated by ligation of integrin or antigen receptor on human B cells". J. Biol. Chem. 272 (7): 4230–6. doi:10.1074/jbc.272.7.4230. PMID 9020138. 
• Tachibana K, Urano T, Fujita H, et al. (1997). "Tyrosine phosphorylation of Crk-associated substrates by focal adhesion kinase. A putative mechanism for the integrin-mediated tyrosine phosphorylation of Crk-associated substrates". J. Biol. Chem. 272 (46): 29083–90. doi:10.1074/jbc.272.46.29083. PMID 9360983. 
• Hunter AJ, Shimizu Y (1997). "Alpha 4 beta 1 integrin-mediated tyrosine phosphorylation in human T cells: characterization of Crk- and Fyn-associated substrates (pp105, pp115, and human enhancer of filamentation-1) and integrin-dependent activation of p59fyn1". J. Immunol. 159 (10): 4806–14. PMID 9366405. 
• de Jong R, van Wijk A, Haataja L, et al. (1998). "BCR/ABL-induced leukemogenesis causes phosphorylation of Hef1 and its association with Crkl". J. Biol. Chem. 272 (51): 32649–55. doi:10.1074/jbc.272.51.32649. PMID 9405482. 
• Ohashi Y, Tachibana K, Kamiguchi K, et al. (1998). "T cell receptor-mediated tyrosine phosphorylation of Cas-L, a 105-kDa Crk-associated substrate-related protein, and its association of Crk and C3G". J. Biol. Chem. 273 (11): 6446–51. doi:10.1074/jbc.273.11.6446. PMID 9497377. 
• Astier A, Manié SN, Law SF, et al. (1998). "Association of the Cas-like molecule HEF1 with CrkL following integrin and antigen receptor signaling in human B-cells: potential relevance to neoplastic lymphohematopoietic cells". Leuk. Lymphoma 28 (1–2): 65–72. doi:10.3109/10428199709058332. PMID 9498705. 
• Law SF, Zhang YZ, Klein-Szanto AJ, Golemis EA (1998). "Cell cycle-regulated processing of HEF1 to multiple protein forms differentially targeted to multiple subcellular compartments". Mol. Cell. Biol. 18 (6): 3540–51. PMC 108935. PMID 9584194. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=108935. 
• Côté JF, Charest A, Wagner J, Tremblay ML (1998). "Combination of gene targeting and substrate trapping to identify substrates of protein tyrosine phosphatases using PTP-PEST as a model". Biochemistry 37 (38): 13128–37. doi:10.1021/bi981259l. PMID 9748319. 
• Kyono WT, de Jong R, Park RK, et al. (1998). "Differential interaction of Crkl with Cbl or C3G, Hef-1, and gamma subunit immunoreceptor tyrosine-based activation motif in signaling of myeloid high affinity Fc receptor for IgG (Fc gamma RI)". J. Immunol. 161 (10): 5555–63. PMID 9820532. 
• Zhang Z, Hernandez-Lagunas L, Horne WC, Baron R (1999). "Cytoskeleton-dependent tyrosine phosphorylation of the p130(Cas) family member HEF1 downstream of the G protein-coupled calcitonin receptor. Calcitonin induces the association of HEF1, paxillin, and focal adhesion kinase". J. Biol. Chem. 274 (35): 25093–8. doi:10.1074/jbc.274.35.25093. PMID 10455189. 
• Ohashi Y, Iwata S, Kamiguchi K, Morimoto C (1999). "Tyrosine phosphorylation of Crk-associated substrate lymphocyte-type is a critical element in TCR- and beta 1 integrin-induced T lymphocyte migration". J. Immunol. 163 (7): 3727–34. PMID 10490968. 
• Law SF, Zhang YZ, Fashena SJ, et al. (1999). "Dimerization of the docking/adaptor protein HEF1 via a carboxy-terminal helix-loop-helix domain". Exp. Cell Res. 252 (1): 224–35. doi:10.1006/excr.1999.4609. PMID 10502414. 
• Scanlan MJ, Gordan JD, Williamson B, et al. (1999). "Antigens recognized by autologous antibody in patients with renal-cell carcinoma". Int. J. Cancer 83 (4): 456–64. doi:10.1002/(SICI)1097-0215(19991112)83:4<456::AID-IJC4>3.0.CO;2-5. PMID 10508479. 
• Sakakibara A, Hattori S (2000). "Chat, a Cas/HEF1-associated adaptor protein that integrates multiple signaling pathways". J. Biol. Chem. 275 (9): 6404–10. doi:10.1074/jbc.275.9.6404. PMID 10692442. 
• Law SF, O'Neill GM, Fashena SJ, et al. (2000). "The docking protein HEF1 is an apoptotic mediator at focal adhesion sites". Mol. Cell. Biol. 20 (14): 5184–95. doi:10.1128/MCB.20.14.5184-5195.2000. PMC 85967. PMID 10866674. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=85967.