Tight junction protein 1

Tight junction protein 1 (zona occludens 1)
PDB rendering based on 2h2b.
Available structures PDB 2H2B, 2H2C, 2H3M, 2JWE, 2RCZ, 3CYY, 3LH5
Identifiers
Symbols	TJP1; DKFZp686M05161; MGC133289; ZO-1
External IDs	OMIM: 601009 MGI: 98759 HomoloGene: 2445 GeneCards: TJP1 Gene
Gene Ontology Molecular function • protein binding • protein domain specific binding Cellular component • nucleus • cytoplasm • Golgi apparatus • plasma membrane • plasma membrane • cell-cell adherens junction • tight junction • basolateral plasma membrane • cell junction • apical junction complex • intercellular canaliculus Biological process • blastocyst formation • apoptosis • cellular component disassembly involved in apoptosis • cell-cell junction assembly Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	7082	21872
Ensembl	ENSG00000104067	ENSMUSG00000030516
UniProt	Q07157	Q91YW2
RefSeq (mRNA)	NM_003257.3	NM_009386
RefSeq (protein)	NP_003248.3	NP_033412
Location (UCSC)	Chr 15: 29.99 – 30.25 Mb	Chr 7: 72.44 – 72.52 Mb
PubMed search	[1]	[2]

Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene.^[1]

This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions. The encoded protein may be involved in signal transduction at cell-cell junctions. Two transcript variants encoding distinct isoforms have been identified for this gene.^[2]

Interactions

Tight junction protein 1 has been shown to interact with F11 receptor,^[3][4] Gap junction protein, alpha 1,^[5] MLLT4,^[6] Occludin,^[7][8] TJP3,^[9] GJA3,^[10] KIRREL,^[11][12] Tight junction protein 2^[7][9][13] and GJA8.^[10]

See also

• Tight junction

References

1. ^ Mohandas TK, Chen XN, Rowe LB, Birkenmeier EH, Fanning AS, Anderson JM, Korenberg JR (Feb 1997). "Localization of the tight junction protein gene TJP1 to human chromosome 15q13, distal to the Prader-Willi/Angelman region, and to mouse chromosome 7". Genomics 30 (3): 594–7. doi:10.1006/geno.1995.1281. PMID 8825647. 
2. ^ "Entrez Gene: TJP1 tight junction protein 1 (zona occludens 1)". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=7082. 
3. ^ Ebnet, K; Schulz C U, Meyer Zu Brickwedde M K, Pendl G G, Vestweber D (Sep. 2000). "Junctional adhesion molecule interacts with the PDZ domain-containing proteins AF-6 and ZO-1". J. Biol. Chem. (UNITED STATES) 275 (36): 27979–88. doi:10.1074/jbc.M002363200. ISSN 0021-9258. PMID 10856295. 
4. ^ Ebnet, Klaus; Aurrand-Lions Michel, Kuhn Annegret, Kiefer Friedemann, Butz Stefan, Zander Kerstin, Meyer zu Brickwedde Maria-Katharina, Suzuki Atsushi, Imhof Beat A, Vestweber Dietmar (Oct. 2003). "The junctional adhesion molecule (JAM) family members JAM-2 and JAM-3 associate with the cell polarity protein PAR-3: a possible role for JAMs in endothelial cell polarity". J. Cell. Sci. (England) 116 (Pt 19): 3879–91. doi:10.1242/jcs.00704. ISSN 0021-9533. PMID 12953056. 
5. ^ Giepmans, B N; Moolenaar W H (1998). "The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein". Curr. Biol. (ENGLAND) 8 (16): 931–4. doi:10.1016/S0960-9822(07)00375-2. ISSN 0960-9822. PMID 9707407. 
6. ^ Yamamoto, T; Harada N, Kawano Y, Taya S, Kaibuchi K (May. 1999). "In vivo interaction of AF-6 with activated Ras and ZO-1". Biochem. Biophys. Res. Commun. (UNITED STATES) 259 (1): 103–7. doi:10.1006/bbrc.1999.0731. ISSN 0006-291X. PMID 10334923. 
7. ^ ^{a b} Fanning, A S; Jameson B J, Jesaitis L A, Anderson J M (Nov. 1998). "The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton". J. Biol. Chem. (UNITED STATES) 273 (45): 29745–53. doi:10.1074/jbc.273.45.29745. ISSN 0021-9258. PMID 9792688. 
8. ^ Rao, Radhakrishna K; Basuroy Shyamali, Rao Vijay U, Karnaky Jr Karl J, Gupta Akshay (Dec. 2002). "Tyrosine phosphorylation and dissociation of occludin-ZO-1 and E-cadherin-beta-catenin complexes from the cytoskeleton by oxidative stress". Biochem. J. (England) 368 (Pt 2): 471–81. doi:10.1042/BJ20011804. ISSN 0264-6021. PMC 1222996. PMID 12169098. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1222996. 
9. ^ ^{a b} Wittchen, E S; Haskins J, Stevenson B R (Dec. 1999). "Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3". J. Biol. Chem. (UNITED STATES) 274 (49): 35179–85. doi:10.1074/jbc.274.49.35179. ISSN 0021-9258. PMID 10575001. 
10. ^ ^{a b} Nielsen, Peter A; Baruch Amos, Shestopalov Valery I, Giepmans Ben N G, Dunia Irene, Benedetti E Lucio, Kumar Nalin M (Jun. 2003). "Lens connexins alpha3Cx46 and alpha8Cx50 interact with zonula occludens protein-1 (ZO-1)". Mol. Biol. Cell (United States) 14 (6): 2470–81. doi:10.1091/mbc.E02-10-0637. ISSN 1059-1524. PMC 194895. PMID 12808044. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=194895. 
11. ^ Liu, Gang; Kaw Beenu, Kurfis Jayson, Rahmanuddin Syed, Kanwar Yashpal S, Chugh Sumant S (Jul. 2003). "Neph1 and nephrin interaction in the slit diaphragm is an important determinant of glomerular permeability". J. Clin. Invest. (United States) 112 (2): 209–21. doi:10.1172/JCI18242. ISSN 0021-9738. PMC 164293. PMID 12865409. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=164293. 
12. ^ Huber, Tobias B; Schmidts Miriam, Gerke Peter, Schermer Bernhard, Zahn Anne, Hartleben Björn, Sellin Lorenz, Walz Gerd, Benzing Thomas (Apr. 2003). "The carboxyl terminus of Neph family members binds to the PDZ domain protein zonula occludens-1". J. Biol. Chem. (United States) 278 (15): 13417–21. doi:10.1074/jbc.C200678200. ISSN 0021-9258. PMID 12578837. 
13. ^ Itoh, M; Morita K, Tsukita S (Feb. 1999). "Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin". J. Biol. Chem. (UNITED STATES) 274 (9): 5981–6. doi:10.1074/jbc.274.9.5981. ISSN 0021-9258. PMID 10026224. 

Further reading

• González-Mariscal L, Betanzos A, Avila-Flores A (2000). "MAGUK proteins: structure and role in the tight junction.". Semin. Cell Dev. Biol. 11 (4): 315–24. doi:10.1006/scdb.2000.0178. PMID 10966866. 
• Anderson NL, Anderson NG (2003). "The human plasma proteome: history, character, and diagnostic prospects.". Mol. Cell Proteomics 1 (11): 845–67. doi:10.1074/mcp.R200007-MCP200. PMID 12488461. 
• Willott E, Balda MS, Heintzelman M, et al. (1992). "Localization and differential expression of two isoforms of the tight junction protein ZO-1.". Am. J. Physiol. 262 (5 Pt 1): C1119–24. PMID 1590354. 
• Van Itallie CM, Balda MS, Anderson JM (1995). "Epidermal growth factor induces tyrosine phosphorylation and reorganization of the tight junction protein ZO-1 in A431 cells.". J. Cell. Sci. 108 ( Pt 4): 1735–42. PMID 7542259. 
• Balda MS, Anderson JM (1993). "Two classes of tight junctions are revealed by ZO-1 isoforms.". Am. J. Physiol. 264 (4 Pt 1): C918–24. PMID 7682777. 
• Furuse M, Itoh M, Hirase T, et al. (1995). "Direct association of occludin with ZO-1 and its possible involvement in the localization of occludin at tight junctions.". J. Cell Biol. 127 (6 Pt 1): 1617–26. doi:10.1083/jcb.127.6.1617. PMC 2120300. PMID 7798316. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2120300. 
• Willott E, Balda MS, Fanning AS, et al. (1993). "The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions.". Proc. Natl. Acad. Sci. U.S.A. 90 (16): 7834–8. doi:10.1073/pnas.90.16.7834. PMC 47237. PMID 8395056. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=47237. 
• Itoh M, Nagafuchi A, Yonemura S, et al. (1993). "The 220-kD protein colocalizing with cadherins in non-epithelial cells is identical to ZO-1, a tight junction-associated protein in epithelial cells: cDNA cloning and immunoelectron microscopy.". J. Cell Biol. 121 (3): 491–502. doi:10.1083/jcb.121.3.491. PMC 2119563. PMID 8486731. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2119563. 
• Andersson B, Wentland MA, Ricafrente JY, et al. (1996). "A "double adaptor" method for improved shotgun library construction.". Anal. Biochem. 236 (1): 107–13. doi:10.1006/abio.1996.0138. PMID 8619474. 
• Yu W, Andersson B, Worley KC, et al. (1997). "Large-scale concatenation cDNA sequencing.". Genome Res. 7 (4): 353–8. doi:10.1101/gr.7.4.353. PMC 139146. PMID 9110174. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139146. 
• Itoh M, Nagafuchi A, Moroi S, Tsukita S (1997). "Involvement of ZO-1 in cadherin-based cell adhesion through its direct binding to alpha catenin and actin filaments.". J. Cell Biol. 138 (1): 181–92. doi:10.1083/jcb.138.1.181. PMC 2139940. PMID 9214391. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2139940. 
• Haskins J, Gu L, Wittchen ES, et al. (1998). "ZO-3, a novel member of the MAGUK protein family found at the tight junction, interacts with ZO-1 and occludin.". J. Cell Biol. 141 (1): 199–208. doi:10.1083/jcb.141.1.199. PMC 2132714. PMID 9531559. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2132714. 
• Moroi S, Saitou M, Fujimoto K, et al. (1998). "Occludin is concentrated at tight junctions of mouse/rat but not human/guinea pig Sertoli cells in testes.". Am. J. Physiol. 274 (6 Pt 1): C1708–17. PMID 9611137. 
• Giepmans BN, Moolenaar WH (1998). "The gap junction protein connexin43 interacts with the second PDZ domain of the zona occludens-1 protein.". Curr. Biol. 8 (16): 931–4. doi:10.1016/S0960-9822(07)00375-2. PMID 9707407. 
• Katsube T, Takahisa M, Ueda R, et al. (1998). "Cortactin associates with the cell-cell junction protein ZO-1 in both Drosophila and mouse.". J. Biol. Chem. 273 (45): 29672–7. doi:10.1074/jbc.273.45.29672. PMID 9792678. 
• Fanning AS, Jameson BJ, Jesaitis LA, Anderson JM (1998). "The tight junction protein ZO-1 establishes a link between the transmembrane protein occludin and the actin cytoskeleton.". J. Biol. Chem. 273 (45): 29745–53. doi:10.1074/jbc.273.45.29745. PMID 9792688. 
• Itoh M, Morita K, Tsukita S (1999). "Characterization of ZO-2 as a MAGUK family member associated with tight as well as adherens junctions with a binding affinity to occludin and alpha catenin.". J. Biol. Chem. 274 (9): 5981–6. doi:10.1074/jbc.274.9.5981. PMID 10026224. 
• Yamamoto T, Harada N, Kawano Y, et al. (1999). "In vivo interaction of AF-6 with activated Ras and ZO-1.". Biochem. Biophys. Res. Commun. 259 (1): 103–7. doi:10.1006/bbrc.1999.0731. PMID 10334923. 
• Wittchen ES, Haskins J, Stevenson BR (2000). "Protein interactions at the tight junction. Actin has multiple binding partners, and ZO-1 forms independent complexes with ZO-2 and ZO-3.". J. Biol. Chem. 274 (49): 35179–85. doi:10.1074/jbc.274.49.35179. PMID 10575001.