Heterocysts are specialized nitrogen-fixing cells formed by some filamentous
cyanobacteria, such as " Nostoc punctiforme", " Cylindrospermum stagnale" and " Anabaena sperica", during nitrogen starvation. They fix nitrogenfrom dinitrogen (N2) in the air using the enzyme nitrogenase, in order to provide the cells in the filament with nitrogen for biosynthesis. Nitrogenase is inactivated by oxygen, so the heterocyst must create a microanaerobic environment. The heterocysts' unique structure and physiologyrequires a global change in gene expression. For example, heterocysts:
* produce three additional
cell walls, including one of glycolipidthat forms a hydrophobic barrier to oxygen
* produce nitrogenase and other proteins involved in nitrogen fixation
* degrade photosystem II, which produces oxygen
* up-regulate glycolytic enzymes
* produce proteins that scavenge any remaining oxygen
Cyanobacteria usually obtain a fixed carbon (carbohydrate) by
photosynthesis. The lack of photosystemII prevents heterocysts from photosynthesising, so the vegetative cells provide them with carbohydrates, which is thought to be sucrose. The fixed carbon and nitrogen sources are exchanged though channels between the cells in the filament. Heterocysts maintain photosystem I, allowing them to generate ATP by cyclic photophosphorylation.
Single heterocysts develop about every 9-15 cells, producing a one-dimensional pattern along the filament. The interval between heterocysts remains approximately constant even though the cells in the filament are dividing. The bacterial filament can be seen as a multicellular organism with two distinct yet interdependent cell types. Such behaviour is highly unusual in
prokaryotes and may have been the first example of multicellular patterning in evolution. Once a heterocyst has formed, it cannot revert to a vegetative cell, so this differentiation can be seen as a form of apoptosis. Certain heterocyst-forming bacteria can differentiate into spore-like cells called akinetes or motile cells called hormogonia, making them the most phenotyptically versatile of all prokaryotes.
The mechanism of controlling heterocysts is thought to involve the diffusion of an inhibitor of differentiation called patS. Heterocyst formation is inhibited in the presence of a fixed nitrogen source, such as
ammoniumor nitrate. Heterocyst maintenance is dependent on an enzyme called hetN. The bacteria may also enter a symbiotic relationship with certain plants. In such a relationship, the bacteria do not respond to the availability of nitrogen, but to signals produced by the plant. Up to 60% of the cells can become heterocysts, providing fixed nitrogen to the plant in return for fixed carbon.
The cyanobacteria that form heterocysts are divided into the orders
Nostocalesand Stigonematales, which form simple and branching filaments respectively. Together they form a monophyleticgroup, with very low genetic variability.
Wikimedia Foundation. 2010.