Puromycin

Puromycin

chembox new
ImageFile=puromycin.svg
ImageSize=
IUPACName=
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Section1=Chembox Identifiers
CASNo=53-79-2
PubChem=5891
SMILES=CN(C)C1=NC=NC2=C1N=CN2C3C(C(C(O3)CO)NC(=O)C(CC4=CC=C(C=C4)OC)N)O
MeSHName=Puromycin
Section2=Chembox Properties
Formula=C22H29N7O5
MolarMass=471.50956
Appearance=
Density=
MeltingPt=
BoilingPt=
Solubility=
Section3=Chembox Hazards
MainHazards=
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Puromycin is an antibiotic that is a potent inhibitor of translation.

Inhibition of translation

Puromycin is an aminonucleoside antibiotic, derived from the "Streptomyces alboniger" bacterium, that causes premature chain termination during translation taking place in the ribosome. Part of the molecule resembles the 3' end of the aminoacylated tRNA. It enters the A site and transfer to the growing chain, causing premature chain release. The exact mechanism of action is unknown at this time, but, the 3' position contains an amide linkage instead of the normal ester linkage of tRNA, the amide bond makes the molecule much more resistant to hydrolysis and thus causes the ribosome to become stopped.

It is not selective for either prokaryotes or eukaryotes.

Also of note, puromycin is critical in mRNA display as it allows the growing peptide chain to be covalently bonded to its own mRNA template.

Cell culture

Puromycin is used in cell biology as selective agent in cell culture systems. It is toxic to prokaryotic and eukaryotic cells. Resistance to puromycin is conferred by the Pac gene encoding a puromycin N-acetyl-transferase (PAC) that was found in a Streptomyces producer strain. Puromycin is soluble in water (50mg/ml) as colorless solution at 10 mg/ml. Puromycin is stable for one year as solution when stored at -20°C. The recommended dose as selection agent in cell culture is at a range of 10-100 μg/ml, although it can be toxic to eukaryotic cells at concentrations as low as 1 μg/ml. It acts quickly and can kill up to 99% of nonresistant cells within 2 days

External links

* [http://www.fermentek.co.il/puromycin.htm Puromycin ] from Fermentek


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Look at other dictionaries:

  • puromycin — puromycin. См. пуромицин. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • puromycin — ☆ puromycin [pyo͞o΄rō mī′sin, pyoor΄ōmī sin ] n. 〚/span> PUR(INE) + O + MYCIN〛 an antibiotic, C22H29N7O5, produced by a soil actinomycete ( Streptomyces alboniger), effective against various parasites, bacteria, etc.: it interferes …   Universalium

  • puromycin — ☆ puromycin [pyo͞o΄rō mī′sin, pyoor΄ōmī sin ] n. [< PUR(INE) + O + MYCIN] an antibiotic, C22H29N7O5, produced by a soil actinomycete ( Streptomyces alboniger), effective against various parasites, bacteria, etc.: it interferes with protein… …   English World dictionary

  • Puromycin — Strukturformel Allgemeines Freiname Puromycin Andere Namen Purimycin …   Deutsch Wikipedia

  • puromycin — noun Etymology: purine + o + mycin Date: 1953 an antibiotic C22H29N7O5 that is obtained from an actinomycete (Streptomyces alboniger) and is a potent inhibitor of protein synthesis …   New Collegiate Dictionary

  • puromycin — An antibiotic that acts as an aminoacyl tRNA analogue. Binds to the A site on the ribosome, forms a peptide linkage with the growing chain and then causes premature termination …   Dictionary of molecular biology

  • puromycin — noun An antibiotic that is a strong inhibitor of protein translation, having the chemical formula CHNO …   Wiktionary

  • puromycin — An antibiotic produced by the growth of Streptomyces alboniger; formerly used in the treatment of amebiasis and trypanosomiasis. * * * pu·ro·my·cin .pyu̇r ə mīs ən n an antibiotic C22H29N7O5 that is obtained from an actinomycete of the genus… …   Medical dictionary

  • puromycin — pu·ro·my·cin …   English syllables

  • puromycin — |pyu̇rə|mīsən noun ( s) Etymology: purine + o + mycin : a trypanocidal and amebicidal antibiotic C22H29N7O5 obtained from an actinomycete (Streptomyces alboniger) …   Useful english dictionary

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