- Apaf-1
Apaf-1 (apoptotic protease activating factor 1) is a cytosolic protein involved in cell death or
apoptosis . WhenCytochrome c is released from themitochondria , it interacts with Apaf-1 and dATP to form theapoptosome , a large oligomeric protein complex which can activatecaspase 9.The
crystal structure of this protein was solved in April 2005 by the laboratory ofYigong Shi [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=15829969] . It contains aGreek Key Motif composed of six helices and 94 amino acids.The actual mechanism for this reaction is still debated though work published in April of
2006 from thelaboratory ofGuy Salvesen suggests that the apoptosome may induce caspase 9 dimerization and subsequentautocatalyzation [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=16630894] .
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