Coilin

Coilin
Identifiers
Symbols	COIL; CLN80; p80-coilin
External IDs	OMIM: 600272 MGI: 104842 HomoloGene: 3413 GeneCards: COIL Gene
Gene Ontology Molecular function • protein binding • protein C-terminus binding Cellular component • nucleus • nucleoplasm • nucleolus • Cajal body Sources: Amigo / QuickGO
RNA expression pattern
More reference expression data
Orthologs
Species	Human	Mouse
Entrez	8161	12812
Ensembl	ENSG00000121058	ENSMUSG00000033983
UniProt	P38432	n/a
RefSeq (mRNA)	NM_004645.2	NM_016706.2
RefSeq (protein)	NP_004636.1	NP_057915.2
Location (UCSC)	Chr 17: 55.02 – 55.04 Mb	Chr 11: 88.83 – 88.85 Mb
PubMed search	[1]	[2]

Coilin is a protein that in humans is encoded by the COIL gene.^[1][2]

Coilin protein is one of the main molecular components of Cajal bodies (CBs). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. In addition to its structural role, coilin acts as glue to connect the CB to the nucleolus. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14.^[2]

To study CBs, coilin can be combined with GFP (Green Fluorescent Protein) to form Colin-GFP hybrid protein. The hybrid protein can then be used to locate CBs underneath a microscope, usually near the nucleolus of the cell. Other proteins that make up the CB include snRNPs and nucleolar snoRNPs.

Coilin got its name from the coiled shaped of the CB in which it is found. It was first identified using human autoimmune serum.

Interactions

Coilin has been shown to interact with SMN1,^[3][4] SNRPB,^[4] Nucleolar phosphoprotein p130^[5] and Ataxin 1.^[6][7]

References

1. ^ Chan EK, Takano S, Andrade LE, Hamel JC, Matera AG (Dec 1994). "Structure, expression and chromosomal localization of human p80-coilin gene". Nucleic Acids Res 22 (21): 4462–9. doi:10.1093/nar/22.21.4462. PMC 308480. PMID 7971277. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=308480. 
2. ^ ^{a b} "Entrez Gene: COIL coilin". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=8161. 
3. ^ Hebert, Michael D; Shpargel Karl B, Ospina Jason K, Tucker Karen E, Matera A Gregory (Sep. 2002). "Coilin methylation regulates nuclear body formation". Dev. Cell (United States) 3 (3): 329–37. doi:10.1016/S1534-5807(02)00222-8. ISSN 1534-5807. PMID 12361597. 
4. ^ ^{a b} Hebert, M D; Szymczyk P W, Shpargel K B, Matera A G (Oct. 2001). "Coilin forms the bridge between Cajal bodies and SMN, the spinal muscular atrophy protein". Genes Dev. (United States) 15 (20): 2720–9. doi:10.1101/gad.908401. ISSN 0890-9369. PMC 312817. PMID 11641277. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=312817. 
5. ^ Isaac, C; Yang Y, Meier U T (Jul. 1998). "Nopp140 functions as a molecular link between the nucleolus and the coiled bodies". J. Cell Biol. (UNITED STATES) 142 (2): 319–29. doi:10.1083/jcb.142.2.319. ISSN 0021-9525. PMC 2133063. PMID 9679133. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2133063. 
6. ^ Hong, Sunghoi; Ka Sojeong, Kim Sungjo, Park Yongjae, Kang Seongman (May. 2003). "p80 coilin, a coiled body-specific protein, interacts with ataxin-1, the SCA1 gene product". Biochim. Biophys. Acta (Netherlands) 1638 (1): 35–42. ISSN 0006-3002. PMID 12757932. 
7. ^ Hong, Sunghoi; Lee Soyeon, Cho Ssang-Goo, Kang Seongman (Jun. 2008). "UbcH6 interacts with and ubiquitinates the SCA1 gene product ataxin-1". Biochem. Biophys. Res. Commun. (United States) 371 (2): 256–60. doi:10.1016/j.bbrc.2008.04.066. PMID 18439907. 

Further reading

• Ogg SC, Lamond AI (October 14, 2002). "Cajal bodies and coilin — moving towards function". J. Cell Biol. 159 (1): 17–21. doi:10.1083/jcb.200206111. PMC 2173504. PMID 12379800. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2173504. 
• Andrade LE, Chan EK, Raska I et al. (1991). "Human autoantibody to a novel protein of the nuclear coiled body: immunological characterization and cDNA cloning of p80-coilin". J. Exp. Med. 173 (6): 1407–19. doi:10.1084/jem.173.6.1407. PMC 2190846. PMID 2033369. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2190846. 
• Bohmann K, Ferreira JA, Lamond AI (1996). "Mutational analysis of p80 coilin indicates a functional interaction between coiled bodies and the nucleolus". J. Cell Biol. 131 (4): 817–31. doi:10.1083/jcb.131.4.817. PMC 2200013. PMID 7490287. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2200013. 
• Carmo-Fonseca M, Ferreira J, Lamond AI (1993). "Assembly of snRNP-containing coiled bodies is regulated in interphase and mitosis--evidence that the coiled body is a kinetic nuclear structure". J. Cell Biol. 120 (4): 841–52. doi:10.1083/jcb.120.4.841. PMC 2200076. PMID 7679389. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2200076. 
• Robertson NG, Khetarpal U, Gutiérrez-Espeleta GA et al. (1995). "Isolation of novel and known genes from a human fetal cochlear cDNA library using subtractive hybridization and differential screening". Genomics 23 (1): 42–50. doi:10.1006/geno.1994.1457. PMID 7829101. 
• Kusuda J, Tanuma R, Gotoh E et al. (1995). "Assignment of a human autoimmune antigen, p80-coilin gene to chromosome 17q21-q23 and of its possible pseudogene to chromosome 14". Hum. Genet. 95 (2): 233–4. doi:10.1007/BF00209411. PMID 7860074. 
• Lyon CE, Bohmann K, Sleeman J, Lamond AI (1997). "Inhibition of protein dephosphorylation results in the accumulation of splicing snRNPs and coiled bodies within the nucleolus". Exp. Cell Res. 230 (1): 84–93. doi:10.1006/excr.1996.3380. PMID 9013710. 
• Parfenov VN, Davis DS, Pochukalina GN et al. (1998). "Dynamics of distribution of splicing components relative to the transcriptional state of human oocytes from antral follicles". J. Cell. Biochem. 69 (1): 72–80. doi:10.1002/(SICI)1097-4644(19980401)69:1<72::AID-JCB8>3.0.CO;2-S. PMID 9513048. 
• Pochukalina GN, Davis DS, Kostiuchek DF et al. (1998). "[Splicing factors in oocyte nuclei from human antral follicles]". Tsitologiia 40 (4): 239–47. PMID 9644758. 
• Isaac C, Yang Y, Meier UT (1998). "Nopp140 functions as a molecular link between the nucleolus and the coiled bodies". J. Cell Biol. 142 (2): 319–29. doi:10.1083/jcb.142.2.319. PMC 2133063. PMID 9679133. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2133063. 
• Liu J, Hebert MD, Ye Y et al. (2000). "Cell cycle-dependent localization of the CDK2-cyclin E complex in Cajal (coiled) bodies". J. Cell. Sci. 113 ( Pt 9): 1543–52. PMID 10751146. 
• Hebert MD, Matera AG (2001). "Self-association of coilin reveals a common theme in nuclear body localization". Mol. Biol. Cell 11 (12): 4159–71. PMC 15064. PMID 11102515. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=15064. 
• Young PJ, Le TT, Dunckley M et al. (2001). "Nuclear gems and Cajal (coiled) bodies in fetal tissues: nucleolar distribution of the spinal muscular atrophy protein, SMN". Exp. Cell Res. 265 (2): 252–61. doi:10.1006/excr.2001.5186. PMID 11302690. 
• Hebert MD, Szymczyk PW, Shpargel KB, Matera AG (2001). "Coilin forms the bridge between Cajal bodies and SMN, the spinal muscular atrophy protein". Genes Dev. 15 (20): 2720–9. doi:10.1101/gad.908401. PMC 312817. PMID 11641277. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=312817. 
• Andersen JS, Lyon CE, Fox AH et al. (2002). "Directed proteomic analysis of the human nucleolus". Curr. Biol. 12 (1): 1–11. doi:10.1016/S0960-9822(01)00650-9. PMID 11790298. 
• Sleeman JE, Ajuh P, Lamond AI (2002). "snRNP protein expression enhances the formation of Cajal bodies containing p80-coilin and SMN". J. Cell. Sci. 114 (Pt 24): 4407–19. PMID 11792806. 
• Hebert MD, Shpargel KB, Ospina JK et al. (2002). "Coilin methylation regulates nuclear body formation". Dev. Cell 3 (3): 329–37. doi:10.1016/S1534-5807(02)00222-8. PMID 12361597. 
• Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Shpargel KB, Ospina JK, Tucker KE et al. (2003). "Control of Cajal body number is mediated by the coilin C-terminus". J. Cell. Sci. 116 (Pt 2): 303–12. doi:10.1242/jcs.00211. PMID 12482916. 
• Sleeman JE, Trinkle-Mulcahy L, Prescott AR et al. (2004). "Cajal body proteins SMN and Coilin show differential dynamic behaviour in vivo". J. Cell. Sci. 116 (Pt 10): 2039–50. doi:10.1242/jcs.00400. PMID 12679382. 
• Hearst SM, Gilder AS, Negi SS, Davis MD, George EM, Whittom AA, Toyota CG, Husedzinovic A et al. (2009). "Cajal-body formation correlates with differential coilin phosphorylation in primary and transformed cell lines". J. Cell. Sci. 122 (Pt 11): 1872–81. doi:10.1242/jcs.044040. PMC 2684838. PMID 19435804. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=2684838. 

This article incorporates text from the United States National Library of Medicine, which is in the public domain.